INT100114

Context Info Confidence 0.73 First Reported 2002 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 3 Total Number 3 Disease Relevance 2.02 Pain Relevance 0.31

plasma membrane (Slc12a2)

Anatomy Link Frequency ascending limb 1 optic nerves 1 Slc12a2 (Rattus norvegicus)

Pain Link Frequency Relevance Heat tetrodotoxin 3 96.08 antagonist 4 94.00 imagery 1 46.72 headache 1 33.60 medulla 1 22.12 isoflurane 4 5.00 anesthesia 3 5.00 depression 1 5.00 fibrosis 1 5.00 bradykinin 1 5.00

Disease Link Frequency Relevance Heat Arachnoid Cysts 44 99.84 Cyst 40 96.40 Chronic Renal Failure 124 91.16 Pressure And Volume Under Development 5 75.00 Hypercalcemia 1 60.56 Bartter Syndrome 1 56.56 Head Trauma 1 54.56 Hematoma 3 40.16 Convulsion 1 34.44 Dizziness 1 34.04

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

We also used a monoclonal antibody to the NKCC1 form of the Na-K-2Cl cotransporter to show that NKCC1 is expressed in optic nerves as shown in Western blotting and is colocalized in GFAP immunopositive astrocytes.

Localization (colocalized) of NKCC1 in optic nerves

1) Confidence 0.73 Published 2002 Journal Glia Section Abstract Doc Link 11754210 Disease Relevance 0.18 Pain Relevance 0.31

We have recently described the up-regulation of the CSF-secreting cation chloride co-transporter NKCC1 in AC membranes compared with normal arachnoid [8].

Localization (secreting) of NKCC1 associated with arachnoid cysts

2) Confidence 0.24 Published 2010 Journal Cerebrospinal Fluid Res Section Body Doc Link PMC2898803 Disease Relevance 1.37 Pain Relevance 0

The Na-K-2Cl cotransporter BSC-1, which is localized at the apical plasma membrane domains of medullary and cortical thick ascending limb segments, mediates the apical NaCl transport in these water-impermeable segments (6, 28).

Localization (localized) of BSC-1 in ascending limb

3) Confidence 0.16 Published 2005 Journal Journal of Korean Medical Science Section Body Doc Link PMC2808601 Disease Relevance 0.47 Pain Relevance 0

General Comments

This test has worked.