INT101831

From wiki-pain
Jump to: navigation, search
Context Info
Confidence 0.77
First Reported 2002
Last Reported 2010
Negated 0
Speculated 2
Reported most in Body
Documents 21
Total Number 27
Disease Relevance 10.82
Pain Relevance 0.12

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

endoplasmic reticulum (KEAP1) nucleolus (KEAP1) nucleus (KEAP1)
cytoplasm (KEAP1)
Anatomy Link Frequency
lung 3
HeLa 1
internal 1
KEAP1 (Homo sapiens)
Pain Link Frequency Relevance Heat
Glutamate 21 99.20 Very High Very High Very High
cINOD 3 75.04 Quite High
Inflammation 35 24.32 Low Low
palliative 15 5.00 Very Low Very Low Very Low
Osteoarthritis 6 5.00 Very Low Very Low Very Low
Pain 6 5.00 Very Low Very Low Very Low
Paracetamol 5 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Cancer 1600 99.84 Very High Very High Very High
Lung Cancer 820 99.40 Very High Very High Very High
Stress 172 91.28 High High
Non-small-cell Lung Cancer 360 88.40 High High
Hyperplasia 15 87.28 High High
Cleidocranial Dysplasia 15 86.24 High High
Carcinoma In Situ 15 85.04 High High
Toxicity 81 83.52 Quite High
Death 120 83.16 Quite High
Malignant Neoplastic Disease 60 81.20 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Importantly, all of the three mutants of KEAP1 could not repress the activity of NRF2, whereas overexpression of WT-KEAP1 completely abolished the NRF2-mediated ARE reporter activity (Figure 6A).
Gene_expression (overexpression) of KEAP1
1) Confidence 0.77 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0.78 Pain Relevance 0
Expression of the wild-type KEAP1 in H838 cells stably expressing an ARE reporter completely abolished ARE reporter activity, whereas overexpression of the KEAP1 mutant construct did not, suggesting that the somatic mutations hamper the association between KEAP1 and NRF2 and consequently activate NRF2.
Gene_expression (overexpression) of KEAP1
2) Confidence 0.77 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0.77 Pain Relevance 0
Expression of the wild-type KEAP1 in H838 cells stably expressing an ARE reporter completely abolished ARE reporter activity, whereas overexpression of the KEAP1 mutant construct did not, suggesting that the somatic mutations hamper the association between KEAP1 and NRF2 and consequently activate NRF2.
Gene_expression (Expression) of KEAP1
3) Confidence 0.77 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0.80 Pain Relevance 0
Conversely, inhibition of KEAP1 expression by siRNA induced the expression of NRF2-driven genes in BEAS2B cells (Figure 6C).


Gene_expression (expression) of KEAP1
4) Confidence 0.77 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0.46 Pain Relevance 0
Consistent with this idea, over-expression of Nrf2 increased GCLC reporter gene expression and over-expression of KIAA0132 inhibited GCLC reporter gene activity as well as inhibited indomethacin-induced increases in the expression of GCLC.
Gene_expression (over-expression) of KIAA0132
5) Confidence 0.73 Published 2002 Journal Free Radic. Biol. Med. Section Abstract Doc Link 11909699 Disease Relevance 0 Pain Relevance 0.08
To confirm the association of Keap1 with SQSTM1, we expressed human Keap1-V5/His or SQSTM1-FLAG/His in HEK293T or HeLa cells and subjected cell lysates to anti-V5 or anti-FLAG pulldown followed by immunoblotting for Keap1 or SQSTM1.
Gene_expression (expressed) of Keap1-V5 in HeLa
6) Confidence 0.71 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
In both cell lines, endogenous SQSTM1 was only present in the anti-V5 pulldowns when Keap1-V5/His was expressed in cells (Fig. 1B).
Gene_expression (expressed) of Keap1-V5
7) Confidence 0.71 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
In the reverse experiment, endogenous Keap1 was only present in the anti-FLAG pulldowns when SQSTM1-FLAG/His was expressed in cells (Fig. 1B).
Gene_expression (present) of Keap1
8) Confidence 0.71 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
Another possible mechanism is differential splicing of KEAP1, leading to nonfunctional KEAP1 protein product in cancer cells.
Gene_expression (product) of KEAP1 protein associated with cancer
9) Confidence 0.67 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0.83 Pain Relevance 0
Detection of KEAP1 alterations in early stages of lung tumor development (stage IB, Table S1) further suggests that loss of KEAP1 function may be an early event in lung cancer pathogenesis.
Gene_expression (function) of KEAP1 in lung associated with lung cancer
10) Confidence 0.67 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 1.34 Pain Relevance 0
, an extremely abundant nuclear oncoprotein, has been recently demonstrated to be an intranuclear dissociator of NRF2–KEAP1 complex and to upregulate expression of NRF2 target genes [36,37].
Gene_expression (expression) of KEAP1
11) Confidence 0.67 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0.77 Pain Relevance 0
Two pairs of fluorescently labeled microsatellite primers flanking KEAP1 (KEAP-UM1 [CA17], present upstream of the KEAP1 locus, and KEAP-DM1 [CA21], present downstream of the KEAP1 locus) were designed using Primer3 software and synthesized by Integrated DNA Technologies.
Gene_expression (synthesized) of KEAP-UM1
12) Confidence 0.67 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0.23 Pain Relevance 0
Two pairs of fluorescently labeled microsatellite primers flanking KEAP1 (KEAP-UM1 [CA17], present upstream of the KEAP1 locus, and KEAP-DM1 [CA21], present downstream of the KEAP1 locus) were designed using Primer3 software and synthesized by Integrated DNA Technologies.
Gene_expression (present) of KEAP-UM1
13) Confidence 0.67 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0.23 Pain Relevance 0
Thus, more intensive studies are needed to unravel the mechanisms responsible for exaggerated NRF2 response in cancer cells even in the presence of wild-type KEAP1.
Gene_expression (presence) of KEAP1 associated with cancer
14) Confidence 0.67 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 1.05 Pain Relevance 0
Detection of KEAP1 alterations in early stages of lung tumor development (stage IB, Table S1) further suggests that loss of KEAP1 function may be an early event in lung cancer pathogenesis.
Gene_expression (Detection) of KEAP1 in lung associated with lung cancer
15) Confidence 0.67 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 1.34 Pain Relevance 0
Two pairs of fluorescently labeled microsatellite primers flanking KEAP1 (KEAP-UM1 [CA17], present upstream of the KEAP1 locus, and KEAP-DM1 [CA21], present downstream of the KEAP1 locus) were designed using Primer3 software and synthesized by Integrated DNA Technologies.
Gene_expression (locus) of KEAP1
16) Confidence 0.67 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0.22 Pain Relevance 0
Plasmid encoding human KEAP1 cDNA in pCMV6-XL5 was purchased from Origene Technologies (Rockville, Maryland, United States).
Gene_expression (cDNA) of KEAP1
17) Confidence 0.67 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0 Pain Relevance 0
To determine whether KEAP1 mutations correlated with NRF2 activation, we performed immunoblot analysis to examine the nuclear accumulation of NRF2.
Spec (whether) Gene_expression (mutations) of KEAP1
18) Confidence 0.67 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0.78 Pain Relevance 0
Sequencing of KEAP1 in 40 primary lung tumors and 16 PF samples from lung cancer patients revealed deletion, insertion, missense, and frameshift mutations in KEAP1 in a total of ten tumors, and the frequency of mutations in PTs and PF was 15% (6/40) and 25% (4/16), respectively.
Gene_expression (Sequencing) of KEAP1 in lung associated with lung cancer and cancer
19) Confidence 0.67 Published 2006 Journal PLoS Medicine Section Body Doc Link PMC1584412 Disease Relevance 0.56 Pain Relevance 0
Furthermore, RNA interference (RNAi) depletion of SQSTM1 resulted in an increase in the protein level of Keap1 and a concomitant decrease in the protein level of Nrf2 in the absence of changes in Keap1 or Nrf2 mRNA levels.
Gene_expression (levels) of Keap1
20) Confidence 0.61 Published 2010 Journal The Journal of Biological Chemistry Section Abstract Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.

Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox