INT103362
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Lbx1 is expressed in and required for the correct specification of three early dorsal interneuron populations and late-born neurons that form the substantia gelatinosa. | |||||||||||||||
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During development, these two major neuronal classes are delineated by the expression of the homeodomain transcription factor Lbx1. | |||||||||||||||
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Similarly, most of the genes in Table 2 appeared to be expressed within the zone of Lbx1 expression, consistent with the idea that they are activated by Lbx1. | |||||||||||||||
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Approximately 20% of the analyzed cells came from early populations (dI4-dI6) and had therefore expressed Lbx1 for more than 24 hours, which may be sufficient for secondary target regulation. | |||||||||||||||
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Heterozygous versus mutant comparisons gave markedly different results in green cells (Fig. 2A), which represent populations that normally express Lbx1, and white cells (Fig. 2B), which represent populations that normally do not express Lbx1. | |||||||||||||||
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Thus, it is even possible that some of these target genes function in limb muscle precursors, which also express Lbx1. | |||||||||||||||
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Given that the set of Lbx1 targets differs between known populations, it is reasonable to assume that each network state in which Lbx1 is expressed has a different set of Lbx1 targets. | |||||||||||||||
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The genes corresponding to these probe sets were not included in the tables, but potentially have Lbx1-dependent expression. | |||||||||||||||
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Most of the genes in Table 1 appeared to be expressed outside of the zone of Lbx1 expression, consistent with the idea that they are repressed by Lbx1. | |||||||||||||||
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Thus, comparisons between mutant and heterozygous sources preferentially reveal immediate molecular consequences of Lbx1 expression, rather than delayed secondary effects. | |||||||||||||||
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The homeodomain transcription factor Lbx1 is expressed in five such populations and loss of Lbx1 leads to distinct respecifications in each of the five populations.
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Heterozygous versus mutant comparisons gave markedly different results in green cells (Fig. 2A), which represent populations that normally express Lbx1, and white cells (Fig. 2B), which represent populations that normally do not express Lbx1. | |||||||||||||||
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Second, these two populations have expressed Lbx1, or GFP, for less than 24 hours. | |||||||||||||||
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Approximately 80% of Lbx1 expressing neurons in E12.5 neural tubes belong to the two late populations. | |||||||||||||||
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In contrast, little scatter from the unity line was observed in the white cell comparison, indicating that few SSTF genes are regulated in cells where Lbx1 is not expressed. | |||||||||||||||
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Foxd3 and Isl1 are normally expressed in postmitotic populations that do not express Lbx1. | |||||||||||||||
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They are normally not expressed in any of the Lbx1 expressing populations. | |||||||||||||||
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The significant increase in the expression levels of these Hox genes in Lbx1 mutants indicates that Lbx1 represses the expression of these Hox genes in at least some dorsal cell populations. | |||||||||||||||
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The probe set for Lhx5 produced a robust, but Lbx1-independent, signal. | |||||||||||||||
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Given that the set of Lbx1 targets differs between known populations, it is reasonable to assume that each network state in which Lbx1 is expressed has a different set of Lbx1 targets. | |||||||||||||||
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General Comments
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