INT104134

Context Info Confidence 0.34 First Reported 2002 Last Reported 2004 Negated 0 Speculated 1 Reported most in Abstract Documents 3 Total Number 4 Disease Relevance 0.52 Pain Relevance 2.75

transmembrane transporter activity (SLC22A6) plasma membrane (SLC22A6) protein complex (SLC22A6) transmembrane transport (SLC22A6)

Anatomy Link Frequency oocytes 1 SLC22A6 (Homo sapiens)

Pain Link Frequency Relevance Heat methotrexate 27 99.76 cINOD 23 99.76 diclofenac 1 89.60 Paracetamol 1 87.92

Disease Link Frequency Relevance Heat Disease 2 95.36 INFLAMMATION 5 90.52 Adverse Drug Reaction 1 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Methotrexate-loxoprofen interaction: involvement of human organic anion transporters hOAT1 and hOAT3.

hOAT1 Binding (interaction) of associated with methotrexate

1) Confidence 0.34 Published 2004 Journal Drug Metab. Pharmacokinet. Section Title Doc Link 15548848 Disease Relevance 0.17 Pain Relevance 0.60

In this study, we examined the drug interaction via hOAT1 and hOAT3, using Xenopus laevis oocytes. hOAT1 and hOAT3 mediated the methotrexate transport with low affinity (K(m) of 724.0 muM) and high affinity (K(m) of 17.2 muM), respectively.

hOAT1 Spec (examined) Binding (interaction) of in oocytes associated with methotrexate

2) Confidence 0.33 Published 2004 Journal Drug Metab. Pharmacokinet. Section Abstract Doc Link 15548848 Disease Relevance 0.19 Pain Relevance 0.57

In addition, hOAT1, hOAT3, and hOAT4 are the sites of drug interactions between methotrexate and NSAIDs, probenecid, and penicillin G.

hOAT1 Binding (interactions) of associated with cinod and methotrexate

3) Confidence 0.08 Published 2002 Journal J. Pharmacol. Exp. Ther. Section Abstract Doc Link 12130730 Disease Relevance 0 Pain Relevance 0.48

By comparing the IC(50) values of NSAIDs for hOATs, it was found that hOAT1 and hOAT3 exhibited higher affinity interactions with NSAIDs than did hOAT2 and hOAT4.

hOAT1 Binding (interactions) of associated with cinod

4) Confidence 0.08 Published 2002 Journal J. Pharmacol. Exp. Ther. Section Abstract Doc Link 12388633 Disease Relevance 0.16 Pain Relevance 1.10

General Comments

This test has worked.