INT105669

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Context Info
Confidence 0.49
First Reported 2002
Last Reported 2010
Negated 0
Speculated 1
Reported most in Body
Documents 20
Total Number 23
Disease Relevance 9.02
Pain Relevance 2.06

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
liver 4
neurons 4
red nucleus 2
subthalamic nucleus 2
epithelial cells 2
AAVS1 (Homo sapiens)
Pain Link Frequency Relevance Heat
Neuropeptide 36 99.84 Very High Very High Very High
Lasting pain 2 98.00 Very High Very High Very High
Spinal cord 28 97.92 Very High Very High Very High
Neuropathic pain 3 97.00 Very High Very High Very High
gABA 2 96.32 Very High Very High Very High
Substantia nigra 39 96.00 Very High Very High Very High
Eae 4 95.64 Very High Very High Very High
Pain 6 94.52 High High
amygdala 24 94.12 High High
Sciatic nerve 1 94.00 High High
Disease Link Frequency Relevance Heat
Targeted Disruption 90 100.00 Very High Very High Very High
Toxicity 69 99.40 Very High Very High Very High
Cytomegalovirus Infection 108 99.32 Very High Very High Very High
Aids-related Complex 135 98.94 Very High Very High Very High
Infection 71 98.94 Very High Very High Very High
Hyperlipoproteinemia Type Ii 264 98.92 Very High Very High Very High
Pain 3 98.00 Very High Very High Very High
Neuropathic Pain 4 97.00 Very High Very High Very High
Body Weight 26 95.88 Very High Very High Very High
Injury 40 94.80 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
To test this hypothesis, we employed adeno-associated virus serotype 2 (AAV2) to selectively over-express human ?
Positive_regulation (employed) of Gene_expression (employed) of AAV
1) Confidence 0.49 Published 2010 Journal Mol Neurodegener Section Abstract Doc Link PMC2975641 Disease Relevance 0.42 Pain Relevance 0.24
Similarly, transfection with an AAV encoding the gene for glutamic acid decarboxylase, synthesizing the inhibitory transmitter GABA has been shown to reduce the excitatory output from the subthalamic nucleus, improving motor cortex activity and consequently in both rodent and macaques models of PD restoring motor function (Luo et al 2002; Lee et al 2005; Emborg et al 2007).


Positive_regulation (transfection) of Gene_expression (transfection) of AAV in subthalamic nucleus associated with gaba and disease
2) Confidence 0.29 Published 2008 Journal Neuropsychiatric Disease and Treatment Section Body Doc Link PMC2626922 Disease Relevance 0.09 Pain Relevance 0.37
AAV-mediated overexpression of neuropeptide Y, agouti and agouti-related peptide in the hypothalamus increased parameters such as body weight and food intake [36,39-41].
Positive_regulation (overexpression) of Gene_expression (overexpression) of AAV in body associated with body weight and neuropeptide
3) Confidence 0.20 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC2912914 Disease Relevance 0.25 Pain Relevance 0.23
region of the forward terminal repeat of AAV results in generation of an AAV containing both complementary strands [21,22].
Positive_regulation (results) of Gene_expression (generation) of AAV
4) Confidence 0.16 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2793898 Disease Relevance 0.24 Pain Relevance 0
In summary, modifying the AAV vector system by altering the capsid (reviewed in [160]), including dsDNA and using a liver specific promoter may result in long term, stable and liver specific AAV mediated transgenic protein expression which may be suitable for FH gene therapy.


Spec (may) Positive_regulation (result) of Gene_expression (expression) of AAV in liver associated with targeted disruption and hyperlipoproteinemia type ii
5) Confidence 0.15 Published 2010 Journal Int Arch Med Section Body Doc Link PMC3016243 Disease Relevance 1.50 Pain Relevance 0.10
A recent study found that administration of Ad 10-20 weeks after AAV gene transfer augmented AAV transgene expression two-fold by increasing the level of pro-viral mRNA [149] and this strategy may prove useful in clinical practice when transgenic protein expression levels fall.
Positive_regulation (transfer) of Gene_expression (transfer) of AAV associated with targeted disruption
6) Confidence 0.15 Published 2010 Journal Int Arch Med Section Body Doc Link PMC3016243 Disease Relevance 0.20 Pain Relevance 0
In summary, modifying the AAV vector system by altering the capsid (reviewed in [160]), including dsDNA and using a liver specific promoter may result in long term, stable and liver specific AAV mediated transgenic protein expression which may be suitable for FH gene therapy.


Positive_regulation (mediated) of Gene_expression (expression) of AAV in liver associated with targeted disruption and hyperlipoproteinemia type ii
7) Confidence 0.15 Published 2010 Journal Int Arch Med Section Body Doc Link PMC3016243 Disease Relevance 1.48 Pain Relevance 0.10
We have investigated the effects of recombinant adeno-associated viral (rAAV)-mediated over-expression of brain-derived neurotrophic factor (BDNF) in the spinal cord on chronic neuropathic pain after unilateral chronic constriction injury (CCI) of the sciatic nerve.
Positive_regulation (over-expression) of Gene_expression (over-expression) of adeno-associated in sciatic nerve associated with eae, injury, neuropathic pain, sciatic nerve and spinal cord
8) Confidence 0.12 Published 2002 Journal Gene Ther. Section Abstract Doc Link 12365004 Disease Relevance 0.93 Pain Relevance 0.85
A recent study found that administration of Ad 10-20 weeks after AAV gene transfer augmented AAV transgene expression two-fold by increasing the level of pro-viral mRNA [149] and this strategy may prove useful in clinical practice when transgenic protein expression levels fall.
Positive_regulation (augmented) of Gene_expression (expression) of AAV associated with targeted disruption
9) Confidence 0.11 Published 2010 Journal Int Arch Med Section Body Doc Link PMC3016243 Disease Relevance 0.21 Pain Relevance 0
All the AAV used for gene therapy studies are transcapsidated: that is to package an AAV genome containing an ITR from one serotype (AAV2) into the capsid of another serotype (i.e.
Positive_regulation (is) of Gene_expression (transcapsidated) of AAV
10) Confidence 0.10 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2660610 Disease Relevance 0.60 Pain Relevance 0
To generate Fto expressing AAV vectors, the cDNA of murine Fto was cloned into an AAV backbone plasmid under the control of the ubiquitous hybrid promoter CAG that contains the chicken ß-actin promoter and CMV enhancer.
Positive_regulation (generate) of Gene_expression (expressing) of AAV associated with cytomegalovirus infection
11) Confidence 0.09 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.19 Pain Relevance 0
Empty AAV vectors expressing no proteins were used as a control for the overexpression study.
Positive_regulation (overexpression) of Gene_expression (overexpression) of AAV
12) Confidence 0.07 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.19 Pain Relevance 0
Thus, rather than manipulating Fto expression in specific ARC neuronal populations, we chose a strategy employing AAV mediated transfer of Fto cDNA and shRNA coupled with stereotactic injection in order to manipulate Fto expression within the ARC (Fig 1C).


Positive_regulation (transfer) of Gene_expression (transfer) of AAV in neuronal associated with aids-related complex
13) Confidence 0.07 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.86 Pain Relevance 0
This adverse tissue response and aberrant cellular morphology was not present in AAV1-GFP transduced neurons (Figure 3c and 4a), indicating that the effect was not due to GFP overexpression or AAV transduction per se.
Positive_regulation (overexpression) of Gene_expression (overexpression) of AAV in neurons
14) Confidence 0.06 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC2841193 Disease Relevance 0 Pain Relevance 0
There was also a trend for rAAV8 GFP expression to be higher than rAAV5 GFP expression (p=0.09).
Positive_regulation (higher) of Gene_expression (expression) of rAAV8
15) Confidence 0.06 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2584774 Disease Relevance 0.22 Pain Relevance 0
After packaging, the resulting AAV1 particles mediate both GFP and shRNA expression.
Positive_regulation (mediate) of Gene_expression (particles) of AAV1
16) Confidence 0.05 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC2841193 Disease Relevance 0.05 Pain Relevance 0
In contrast, AAV1 mediated shRNA expression in the red nucleus triggered an adverse tissue response leading to neuronal degeneration.
Positive_regulation (mediated) of Gene_expression (expression) of AAV1 in red nucleus
17) Confidence 0.05 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC2841193 Disease Relevance 0.63 Pain Relevance 0
Our positive results with rAAV infection of corneal organ cultures suggest that in vivo gene transfer via rAAV may not even necessitate prior epithelial removal.
Positive_regulation (transfer) of Gene_expression (transfer) of rAAV associated with infection
18) Confidence 0.04 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2584774 Disease Relevance 0.21 Pain Relevance 0.09
Statistical analysis showed that rAAV1 yielded significantly higher GFP expression than rAAV5 (2.2 fold; p=0.03), with a trend (p=0.09) for higher expression than rAAV7, but was not significantly different from rAAV2 or rAAV8.
Positive_regulation (higher) of Gene_expression (expression) of rAAV5
19) Confidence 0.04 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2584774 Disease Relevance 0.24 Pain Relevance 0
Transduction of the rabbit corneas with rAAV8 and especially, rAAV1, produced a stronger GFP expression in epithelial cells compared to rAAV5.
Positive_regulation (stronger) of Gene_expression (expression) of rAAV5 in epithelial cells
20) Confidence 0.04 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2584774 Disease Relevance 0.08 Pain Relevance 0

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