INT106525

Context Info Confidence 0.82 First Reported 2003 Last Reported 2011 Negated 1 Speculated 8 Reported most in Body Documents 436 Total Number 444 Disease Relevance 183.81 Pain Relevance 47.82

transport (Akt1) aging (Akt1) enzyme binding (Akt1) carbohydrate metabolic process (Akt1) cytoplasm (Akt1) cytosol (Akt1)

Anatomy Link Frequency muscle 17 nucleus 5 liver 5 adipose tissue 5 HPAC 4 Akt1 (Mus musculus)

Pain Link Frequency Relevance Heat Kinase C 1537 100.00 opioid receptor 1021 100.00 Eae 494 100.00 Clonidine 212 100.00 Morphine 1053 99.96 antidepressant 79 99.84 Delta opioid receptors 33 99.78 agonist 764 99.76 cytokine 1116 99.60 methotrexate 16 99.50

Disease Link Frequency Relevance Heat Obesity 4664 99.96 Apoptosis 2146 99.96 Myeloid Leukemia 317 99.84 Disorder Of Lipid Metabolism 615 99.76 Targeted Disruption 3444 99.56 Stress 1531 99.56 Shock 2119 99.54 Diabetes Mellitus 1576 99.54 Cancer 2410 99.46 Aging 2396 99.44

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

In this immunohistochemical study we have monitored phosphorylated Akt (p-Akt) levels in adult mouse DRGs and spinal cord following unilateral peripheral sciatic nerve transection (axotomy) or carrageenan-induced inflammation.

Phosphorylation (phosphorylated) of Akt in adult mouse associated with inflammation, sciatic nerve and spinal cord

1) Confidence 0.82 Published 2009 Journal Neurosignals Section Abstract Doc Link 19346757 Disease Relevance 0.54 Pain Relevance 0.40

In this immunohistochemical study we have monitored phosphorylated Akt (p-Akt) levels in adult mouse DRGs and spinal cord following unilateral peripheral sciatic nerve transection (axotomy) or carrageenan-induced inflammation.

Phosphorylation (phosphorylated) of Akt in adult mouse associated with inflammation, sciatic nerve and spinal cord

2) Confidence 0.82 Published 2009 Journal Neurosignals Section Abstract Doc Link 19346757 Disease Relevance 0.54 Pain Relevance 0.40

These results strongly suggest that simvastatin improves the endothelial dysfunction seen in type 2 diabetic mice via increases in Akt and Akt phosphorylation.

Phosphorylation (phosphorylation) of Akt associated with diabetes mellitus

3) Confidence 0.82 Published 2008 Journal J. Pharmacol. Sci. Section Abstract Doc Link 18391484 Disease Relevance 0.78 Pain Relevance 0.30

These results strongly suggest that simvastatin improves the endothelial dysfunction seen in type 2 diabetic mice via increases in Akt and Akt phosphorylation.

Phosphorylation (phosphorylation) of Akt associated with diabetes mellitus

4) Confidence 0.82 Published 2008 Journal J. Pharmacol. Sci. Section Abstract Doc Link 18391484 Disease Relevance 0.79 Pain Relevance 0.30

Dexamethasone treatment prevented the decline of mitochondrial potential caused by bupivacaine and increased the levels of Akt phosphorylation.

Phosphorylation (phosphorylation) of Akt associated with dexamethasone

5) Confidence 0.82 Published 2010 Journal Neuroscience Section Abstract Doc Link 20038443 Disease Relevance 0.75 Pain Relevance 0.57

As2O3 inhibits phosphorylation of Akt, mTOR (mammalian target of rapamycin), and p70s6k

Phosphorylation (phosphorylation) of Akt

6) Confidence 0.82 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2920914 Disease Relevance 0 Pain Relevance 0

M methylarsonous acid inhibited insulin-dependent phosphorylation of Akt on both Ser473 and Thr308 residues in 3T3-L1 adipocytes.

Phosphorylation (phosphorylation) of Akt in 3T3-L1 associated with obesity

7) Confidence 0.82 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2920914 Disease Relevance 0.39 Pain Relevance 0

In the present study, we found that a submicromolar concentration of As2O3 significantly and dose-dependently decreased phosphorylation of Akt protein and its downstream targets, mTOR and p70s6k, during myogenic differentiation.

Phosphorylation (phosphorylation) of Akt

8) Confidence 0.82 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2920914 Disease Relevance 0.50 Pain Relevance 0.03

Therefore, we investigated the effects of As2O3 on the phosphorylation of Akt and its downstream signals, mTOR and p70s6k, during myogenic differentiation.

Phosphorylation (phosphorylation) of Akt

9) Confidence 0.82 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2920914 Disease Relevance 0 Pain Relevance 0

M) significantly decreased the phosphorylation of Akt (Figure 5B) and its downstream targets, mTOR and p70s6k proteins, during myogenic differentiation in a dose-dependent manner Figure 6B).

Phosphorylation (phosphorylation) of Akt

10) Confidence 0.82 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2920914 Disease Relevance 0 Pain Relevance 0

As shown in Figure 5B, Akt protein phosphorylation was gradually activated during myogenic differentiation.

Phosphorylation (phosphorylation) of Akt

11) Confidence 0.82 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2920914 Disease Relevance 0 Pain Relevance 0

We observed an increase of myogenin expression and Akt phosphorylation in the soleus muscles on day 3 after glycerol injection, but it was suppressed in As2O3-treated mice compared with control mice (Figure 8).

Phosphorylation (phosphorylation) of Akt in muscles

12) Confidence 0.81 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2920914 Disease Relevance 0.24 Pain Relevance 0.04

Here we report that an acute ethanol challenge induces a robust phosphorylation of two key signal transduction kinases, AKT and DARPP-32, in the striatum of mice.

Phosphorylation (phosphorylation) of AKT in striatum

13) Confidence 0.81 Published 2010 Journal Addict Biol Section Abstract Doc Link 20456289 Disease Relevance 0 Pain Relevance 0.31

Ethanol-induced AKT phosphorylation was blocked by the opioid receptor antagonist naltrexone but unaffected by blockade of dopamine D2 receptors via sulpiride.

Phosphorylation (phosphorylation) of AKT associated with dopamine, antagonist and opioid receptor

14) Confidence 0.81 Published 2010 Journal Addict Biol Section Abstract Doc Link 20456289 Disease Relevance 0 Pain Relevance 0.32

In contrast, we have not observed any modulation of the phosphorylation of TrkB Shc binding site, phosphorylation of mitogen-activated protein kinase or AKT by antidepressants.

Phosphorylation (phosphorylation) of AKT associated with antidepressant

15) Confidence 0.80 Published 2007 Journal Neuropsychopharmacology Section Abstract Doc Link 17314919 Disease Relevance 0 Pain Relevance 0.76

Interestingly, downregulation of phosphorylated PKB/Akt levels by wartmannin resulted in apoptosis of synoviocytes and macrophages in rheumatoid arthritis [33].

Phosphorylation (phosphorylated) of PKB/Akt in macrophages associated with rheumatoid arthritis and apoptosis

16) Confidence 0.80 Published 2006 Journal Arthritis Res Ther Section Body Doc Link PMC1526584 Disease Relevance 1.26 Pain Relevance 0.33

Arvanil blocks the G1/S phase transition of the cell cycle in stimulated peripheral blood mononuclear cells, inducing activation of p21waf-1/cip-1 and phosphorylation of Akt/PKB.

Phosphorylation (phosphorylation) of PKB in mononuclear cells

17) Confidence 0.80 Published 2006 Journal J. Neuroimmunol. Section Abstract Doc Link 16239036 Disease Relevance 0.27 Pain Relevance 0.12

We performed Western blot analysis of the total protein lysate from the tissues of mouse PIN lesions to measure the level of expression of androgen receptor, vascular endothelial growth factor, nuclear factor-kappaB p65, BclII, AKT (total and phosphorylated Ser473), p53, cyclin-dependent kinase inhibitor p21WAF1/CIP1, p27, BAX, and caspase-3 to demonstrate the COX-2-independent mechanism involved in the inhibition of PIN lesions of the dorsolateral prostate by both celecoxib and exisulind.

Phosphorylation (phosphorylated) of AKT

18) Confidence 0.80 Published 2004 Journal Clin. Cancer Res. Section Body Doc Link 15570007 Disease Relevance 0.07 Pain Relevance 0

Arvanil blocks the G1/S phase transition of the cell cycle in stimulated peripheral blood mononuclear cells, inducing activation of p21waf-1/cip-1 and phosphorylation of Akt/PKB.

Phosphorylation (phosphorylation) of Akt in mononuclear cells

19) Confidence 0.80 Published 2006 Journal J. Neuroimmunol. Section Abstract Doc Link 16239036 Disease Relevance 0.27 Pain Relevance 0.12

Vasudevan reported that the tumor suppressor PTEN, which functions as a negative regulator of the phosphorylated Akt-mediated cell survival pathway in cholesteatoma, is downregulated by activated NF-?

Phosphorylation (phosphorylated) of Akt associated with cancer and cholesteatoma

20) Confidence 0.79 Published 2010 Journal Mediators of Inflammation Section Body Doc Link PMC2858283 Disease Relevance 1.61 Pain Relevance 0.12

General Comments

This test has worked.