INT109820

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Context Info
Confidence 0.78
First Reported 2003
Last Reported 2010
Negated 2
Speculated 0
Reported most in Body
Documents 121
Total Number 122
Disease Relevance 39.69
Pain Relevance 33.11

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

transport (Trpv4) plasma membrane (Trpv4) cilium (Trpv4)
transmembrane transport (Trpv4)
Anatomy Link Frequency
neurons 23
MDCK 6
urothelial cells 6
kidney 4
keratinocytes 3
Trpv4 (Mus musculus)
Pain Link Frequency Relevance Heat
dorsal root ganglion 1874 100.00 Very High Very High Very High
TRP channel 1034 100.00 Very High Very High Very High
Spinal cord 346 99.98 Very High Very High Very High
spinal dorsal horn 741 99.90 Very High Very High Very High
nociceptor 238 99.84 Very High Very High Very High
Hippocampus 231 99.68 Very High Very High Very High
Central nervous system 35 99.68 Very High Very High Very High
Calcitonin gene-related peptide 3 99.44 Very High Very High Very High
imagery 277 99.08 Very High Very High Very High
Dorsal horn 88 99.08 Very High Very High Very High
Disease Link Frequency Relevance Heat
Ganglion Cysts 1892 100.00 Very High Very High Very High
Stress 219 100.00 Very High Very High Very High
Helminth Infection 26 100.00 Very High Very High Very High
Increased Venous Pressure Under Development 174 99.32 Very High Very High Very High
Hypersensitivity 113 99.16 Very High Very High Very High
Pain 581 99.00 Very High Very High Very High
Pressure And Volume Under Development 335 98.96 Very High Very High Very High
Benign Prostatic Hypertrophy 10 98.70 Very High Very High Very High
INFLAMMATION 366 98.64 Very High Very High Very High
Disease 215 98.64 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
To elucidate the localization in the mechanosensitive endings, we found with immunohistochemistry in mice that TRPV4 was expressed both by small (low threshold) and large (high threshold) dorsal root ganglia neurons.
Gene_expression (expressed) of TRPV4 in neurons
1) Confidence 0.78 Published 2003 Journal Neurosci. Lett. Section Abstract Doc Link 14665413 Disease Relevance 0 Pain Relevance 0.11
The TRPV4 channel expressed in vitro in Chinese hamster ovary cells was opened by low pH, citrate, and inflation but not by heat or capsaicin.
Gene_expression (expressed) of TRPV4 in ovary associated with qutenza
2) Confidence 0.78 Published 2003 Journal J. Biol. Chem. Section Abstract Doc Link 12692122 Disease Relevance 0.09 Pain Relevance 0.18
The TRPV4 agonists 5',6'-epoxyeicosatrienoic acid and 4alpha-phorbol 12,13-didecanoate stimulated discharge of action potentials in colonic afferent fibers and enhanced current responses recorded from retrogradely labeled colonic dorsal root ganglia neurons, confirming expression of functional TRPV4.
Gene_expression (expression) of TRPV4 in dorsal root ganglia associated with action potential and agonist
3) Confidence 0.78 Published 2008 Journal Am. J. Physiol. Gastrointest. Liver Physiol. Section Abstract Doc Link 18325985 Disease Relevance 0.57 Pain Relevance 0.73
Thus TRPV4 is expressed by primary spinal afferent neurons innervating the colon.
Gene_expression (expressed) of TRPV4 in afferent neurons
4) Confidence 0.78 Published 2008 Journal Am. J. Physiol. Gastrointest. Liver Physiol. Section Abstract Doc Link 18325985 Disease Relevance 0.55 Pain Relevance 0.66
BAA activated a large TRPV4-like current in immortalized mouse keratinocytes (308 cells) that have been shown to express TRPV4 protein endogenously.
Gene_expression (express) of TRPV4 in keratinocytes
5) Confidence 0.77 Published 2006 Journal J. Biol. Chem. Section Abstract Doc Link 16899456 Disease Relevance 0 Pain Relevance 0.14
This compound also activated TRPV4 currents in cell-free outside-out patches from HEK293T cells overexpressing TRPV4 cDNA, suggesting that BAA can activate the channel in a membrane-delimited manner.
Gene_expression (overexpressing) of TRPV4
6) Confidence 0.77 Published 2006 Journal J. Biol. Chem. Section Abstract Doc Link 16899456 Disease Relevance 0 Pain Relevance 0.13
Finally, Miyamoto et al. [26] showed that in patients with benign prostatic hyperplasia or bladder outlet obstruction, bladder urothelium showed higher expressions of TRPA1, TRPV4, and TRPV1, confirming that these channels are candidates as mechanosensors in the human urinary bladder and indicating that modulation of these channels could be a novel therapy for overactive bladder and storage dysfunction.


Gene_expression (expressions) of TRPV4 in urinary bladder associated with benign prostatic hypertrophy and overactive bladder
7) Confidence 0.77 Published 2010 Journal F1000 Biol Rep Section Body Doc Link PMC2948364 Disease Relevance 0.28 Pain Relevance 0.03
TRPV4 is widely expressed throughout the body, including renal epithelium, auditory hair cells, skin keratinocytes, hippocampus neurons, endothelial cells, and urinary bladder epithelium, thereby contributing to numerous physiological processes such as osmoregulation, hearing, thermal and mechanical hyperalgesia, neural activity in the brain, skin barrier recovery, and cell volume regulation.
Gene_expression (expressed) of TRPV4 in brain associated with hyperalgesia and hippocampus
8) Confidence 0.77 Published 2010 Journal F1000 Biol Rep Section Body Doc Link PMC2948364 Disease Relevance 0.43 Pain Relevance 0.14
These authors demonstrated that TRPV4 was expressed mainly in bladder basal and intermediate urothelial cells and functioned as a Ca2+ influx pathway activated by hypotonic cell swelling and induced by the specific TRPV4 agonist 4?
Gene_expression (expressed) of TRPV4 in urothelial cells associated with pressure and volume under development and agonist
9) Confidence 0.77 Published 2010 Journal F1000 Biol Rep Section Body Doc Link PMC2948364 Disease Relevance 0.75 Pain Relevance 0.17
The functional expression of TRPV4 in urothelial cells was evidenced by using the selective agonist 4?
Gene_expression (expression) of TRPV4 in urothelial cells associated with agonist
10) Confidence 0.77 Published 2010 Journal F1000 Biol Rep Section Body Doc Link PMC2948364 Disease Relevance 0 Pain Relevance 0.33
In an immunohistochemical study, TRPV4 was localized in basal and intermediate urothelial cell layers of mice bladders, and TRPV4 expression was also confirmed in primary cell cultures.
Gene_expression (expression) of TRPV4 in urothelial cell
11) Confidence 0.77 Published 2010 Journal F1000 Biol Rep Section Body Doc Link PMC2948364 Disease Relevance 0.05 Pain Relevance 0.33
Immunoreactive TRPV4 and TRPA1 were detected in pancreatic nerve fibers and in dorsal root ganglia neurons innervating the pancreas, which were identified by retrograde tracing.
Gene_expression (detected) of TRPV4 in neurons
12) Confidence 0.77 Published 2010 Journal Am. J. Physiol. Gastrointest. Liver Physiol. Section Abstract Doc Link 20539005 Disease Relevance 0.95 Pain Relevance 0.94
However, flow-induced Ca2+ signals were virtually abolished in the same cells after the induction of TRPV4 siRNA expression by tetracycline (Fig. 3, C, E, G, and H).
Gene_expression (expression) of TRPV4
13) Confidence 0.76 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2500130 Disease Relevance 0.07 Pain Relevance 0
After identifying two siRNA oligonucleotides that efficiently reduced expression of canine TRPV4 (Fig. 3 A), we generated polyclonal MDCK cells expressing TRPV4 siRNA under the control of the TET repressor; the expression of siRNA in individual cells was monitored by the concomitant expression of GFP (Fig. 3, B and C).
Gene_expression (expressing) of TRPV4 in MDCK
14) Confidence 0.76 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2500130 Disease Relevance 0.09 Pain Relevance 0.06
Thus, the increases in swelling-induced conductance are not the result of increased TRPV4 surface expression but rather caused by altered channel properties through formation of TRPP2/TRPV4 heteromers.
Gene_expression (expression) of TRPV4 associated with pressure and volume under development
15) Confidence 0.76 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2500130 Disease Relevance 0.18 Pain Relevance 0.04
Furthermore, robust flow-induced Ca2+ signals were observed in MDCK cells expressing a TRPV4 siRNA with a two-base mismatch, which does not alter TRPV4 expression levels (Fig.
Gene_expression (expressing) of TRPV4 in MDCK
16) Confidence 0.76 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2500130 Disease Relevance 0.19 Pain Relevance 0
After identifying two siRNA oligonucleotides that efficiently reduced expression of canine TRPV4 (Fig. 3 A), we generated polyclonal MDCK cells expressing TRPV4 siRNA under the control of the TET repressor; the expression of siRNA in individual cells was monitored by the concomitant expression of GFP (Fig. 3, B and C).
Gene_expression (expression) of TRPV4 in MDCK
17) Confidence 0.76 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2500130 Disease Relevance 0.10 Pain Relevance 0.07
Interestingly, coexpression of TRPV4 significantly increased TRPP2 surface expression (Fig. 2 G), whereas TRPP2 reduced both TRPV4 surface expression and TRPV4 total protein levels (Fig. 2 I).
Gene_expression (coexpression) of TRPV4
18) Confidence 0.76 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2500130 Disease Relevance 0.29 Pain Relevance 0.03
Noninduced MDCK cells did not express TRPV4 siRNA and consistently displayed Ca2+ signals upon flow stimulation like wild-type MDCK cells (Fig. 3, B, D, and F).
Neg (not) Gene_expression (express) of TRPV4 in MDCK
19) Confidence 0.76 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2500130 Disease Relevance 0.08 Pain Relevance 0.03
To address the question of whether the TRPP2 effect on swelling-activated currents is simply mediated through increased surface expression of TRPV4, we inserted a V5 tag into the first extracellular loop of TRPV4 and TRPP2, respectively, and monitored either ion channel at the cell surface using a modified ELISA system (Wegierski et al., 2006).
Gene_expression (expression) of TRPV4 associated with pressure and volume under development
20) Confidence 0.76 Published 2008 Journal The Journal of Cell Biology Section Body Doc Link PMC2500130 Disease Relevance 0.40 Pain Relevance 0

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