INT11013
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Total CREB and phosphorylated CREB in both ipsilateral and contralateral dorsal horn neurons were dramatically reduced in antisense ODN injected PSNL rats 1 week after injection. | |||||||||||||||
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Both formalin-evoked CREB phosphorylation and c-Fos expression in the spinal cord were suppressed by pretreatment with the NMDA receptor antagonist MK-801 (3.5 mg/kg, i.p.) or halothane anesthesia. | |||||||||||||||
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KN-62 and staurosporine significantly blocked the opioids- induced CREB phosphorylation, while H-89 and PD 98059 had no effect on the actions. | |||||||||||||||
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Intrathecal treatment with a CaM kinase II inhibitor, KN-93, significantly blocked the increased phosphorylation of CREB, but did not affect the CREB protein itself. | |||||||||||||||
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By contrast, inhibition of protein kinase A (PKA) with 100 microM PKA inhibitor peptide, PKI, blocked A1254-induced CREB phosphorylation. | |||||||||||||||
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Buffering [Ca(2+)](i) with bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester in the absence of extracellular Ca(2+) partially inhibited A1254-induced CREB phosphorylation. | |||||||||||||||
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Western blot analysis showed that the phosphorylation of CREB in the spinal cord of rats increased significantly at the peak stage of EAE compared with the controls (p<0.05) and declined significantly in the recovery stage (p<0.05). | |||||||||||||||
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We show here, by use of a back phosphorylation and immunoprecipitation procedure, that acute morphine decreases the state of phosphorylation of CREB, an effect that becomes completely attenuated after chronic morphine administration. | |||||||||||||||
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In our present study, we found that U0126, a MAP kinase inhibitor, significantly blocked CREB phosphorylation at Ser-133 in response to capsaicin injection. | |||||||||||||||
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In an investigation involving in vitro preparation of cultured striatal neurons, the NMDA- and AMPA/kainate-induced CREB phosphorylation was blocked by MAP kinase inhibitor, U0126 [23]. | |||||||||||||||
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Forskolin-stimulated CGRP release and CREB phosphorylation could be mimicked by incubation of the cells with chlorophenylthio-cAMP and blocked by pretreatment with the PKA inhibitor myrPKI(14-22). | |||||||||||||||
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These experiments indicated that partial inhibition of CREB phosphorylation led to a decrease in the number of of BrdU-immunopositive cells in NMDA plus CREB inhibitor-treated cultures (Figure 4G-I). | |||||||||||||||
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The tyrosine kinase (Trk) blocker K252a (2 microg) significantly reduced the CREB phosphorylation elicited either by BDNF or the sciatic ligation. | |||||||||||||||
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These measurements showed that estradiol increased phosphorylation of CREB (cyclic AMP response-element binding protein), which was consistently blocked by the ERK pathway inhibitor PD98059 but not by the inhibitors of phosphatidylinositol 3-kinase, wortmannin and LY294002. 17beta-Estradiol activation of CREB in DRG neurons was reduced by the ER antagonist, ICI182780. | |||||||||||||||
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The spinal ERK inhibition or knockdown also reduced morphine withdrawal-induced phosphorylation of cAMP response element binding protein (CREB), which is one of the important downstream substrates of ERK pathway, and Fos expression. | |||||||||||||||
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Interestingly, CHX itself increased the phosphorylation of CREB, which was abolished by KA administration. | |||||||||||||||
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The latter scenario gains credence from experiments where immobilizing the Ca2+ channel's C terminus prevented CREB phosphorylation but spared Ca2+ influx (Kobrinsky et al., 2003). | |||||||||||||||
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Phosphorylation of CREB and mechanical hyperalgesia is reversed by blockade of the cAMP pathway in a time-dependent manner after repeated intramuscular acid injections. | |||||||||||||||
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The extent of reduction of total CREB and phosphorylated CREB containing cells in the dorsal horn ipsilateral to injury was greater than in the contralateral dorsal horn. | |||||||||||||||
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CREB phosphorylation was very rapid and reached peak levels within 10 min of formalin treatment, whereas few pCREB-positive neurons were seen in unstimulated spinal cord. | |||||||||||||||
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General Comments
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