INT111081

Context Info Confidence 0.75 First Reported 2002 Last Reported 2010 Negated 2 Speculated 0 Reported most in Body Documents 19 Total Number 45 Disease Relevance 51.09 Pain Relevance 26.64

lyase activity (CA1) Golgi apparatus (CA1) cytoplasm (CA1)

Anatomy Link Frequency neurons 8 synovium 5 synovial fluid 4 neuronal 2 proximal 1 CA1 (Homo sapiens)

Pain Link Frequency Relevance Heat spinal inflammation 2322 99.84 Pyramidal cell 61 99.84 Osteoarthritis 972 99.60 Nav1.6 3 99.52 rheumatoid arthritis 1027 99.06 Chronic pancreatitis 14 98.64 long-term potentiation 110 98.16 Inflammation 231 97.12 Hippocampus 100 92.12 cINOD 108 91.28

Disease Link Frequency Relevance Heat Pancreatitis 16 100.00 Low Back Pain 2295 99.84 Hypercalcemia 297 99.72 Stress 1431 99.62 Frailty 1026 99.60 Uveitis 81 99.36 Rheumatoid Arthritis 1027 99.06 Calcification 159 98.12 Apoptosis 65 97.60 Hyperplasia 82 97.56

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

We thus detected especially high expression of CA1 in the synovial tissues of AS patients.

Gene_expression (expression) of CA1 associated with spinal inflammation

1) Confidence 0.75 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.60 Pain Relevance 1.20

Our results suggest that elevated expression of CA1 in the diseased synovial tissues plays a role in new bone formation and bone resorption.

Gene_expression (expression) of CA1 associated with hypercalcemia

2) Confidence 0.75 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 2.00 Pain Relevance 0.68

Increased CA1 expression in the synovium of AS patients may lead to improper mineralization by accelerating calcium salt deposition.

Gene_expression (expression) of CA1 in synovium associated with spinal inflammation

3) Confidence 0.75 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 0.66 Pain Relevance 0.40

Among the five OA synovial samples, only one sample had a relatively high CA1 expression.

Gene_expression (expression) of CA1 associated with osteoarthritis

4) Confidence 0.75 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.08 Pain Relevance 0.69

Immunohistochemistry detected CA1 expression in all AS synovial tissues (100%) and yielded high intensity signals.

Gene_expression (expression) of CA1 associated with spinal inflammation

5) Confidence 0.75 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.01 Pain Relevance 0.65

Compared to the average level of the OA samples, fluid samples from AS patients had a two-fold or greater increase in CA1 expression in 31 samples (77.5%).

Gene_expression (expression) of CA1 associated with spinal inflammation and osteoarthritis

6) Confidence 0.75 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.56 Pain Relevance 1.09

The increased expression of CA1 was confirmed by individually investigating synovial samples from each disease using western blot analysis.

Gene_expression (expression) of CA1 associated with disease

7) Confidence 0.75 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.90 Pain Relevance 1.24

The high expression levels of CA1 in the synovial tissues of patients with AS may partially explain the strong association of the two diseases.

Gene_expression (expression) of CA1 associated with spinal inflammation and disease

8) Confidence 0.75 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.81 Pain Relevance 0.54

Overexpression of CA1 in the synovium of AS patients may be involved in bone resorption of the disease.

Gene_expression (Overexpression) of CA1 in synovium associated with spinal inflammation, hypercalcemia and disease

9) Confidence 0.75 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.64 Pain Relevance 0.48

Therefore, overexpression of CA1 in the synovium of AS patients may lead to CaCO3 deposition in diseased tissues.

Gene_expression (overexpression) of CA1 in synovium associated with spinal inflammation

10) Confidence 0.75 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 0.51 Pain Relevance 0.35

Using a highly sensitive electron microscopic immunogold technique, we revealed the presence of the Nav1.6 subunit in hippocampal CA1 PC proximal and distal dendrites.

Gene_expression (proximal) of CA1 in proximal associated with nav1.6

11) Confidence 0.65 Published 2010 Journal Science Section Abstract Doc Link 20466935 Disease Relevance 0 Pain Relevance 0.38

Expression of CA1 in synovial fluid

Gene_expression (Expression) of CA1 in synovial fluid

12) Confidence 0.65 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.48 Pain Relevance 1.00

In the present study, a proteomic approach exclusively identified the increased expression of CA1 in AS synovial membranes as compared with RA and OA samples.

Gene_expression (expression) of CA1 associated with spinal inflammation, rheumatoid arthritis and osteoarthritis

13) Confidence 0.65 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.94 Pain Relevance 1.27

Furthermore, they found that CA1 promotes the formation of CaCO3 and the precipitant quickly settles [14].

Gene_expression (promotes) of CA1

14) Confidence 0.65 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 0.50 Pain Relevance 0.42

CA1 was detected in six samples of synovial membranes from OA patients (60%); the enzyme was detected in the upper lining within the thin layer of cells.

Gene_expression (detected) of CA1 in upper associated with osteoarthritis

15) Confidence 0.65 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.28 Pain Relevance 0.88

Among the 40 RA synovial fluid samples, 12 samples had higher levels of CA1 (30%, O.D. > 2.0).

Gene_expression (levels) of CA1 in synovial fluid associated with rheumatoid arthritis

16) Confidence 0.65 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.72 Pain Relevance 1.14

These proteins are Gla protein, osteoprotegerin, fibrillin I and carbonic anhydrase (all produced by vascular smooth muscle cells).

Gene_expression (produced) of carbonic anhydrase in smooth muscle cells

17) Confidence 0.58 Published 2005 Journal BMC Cardiovasc Disord Section Body Doc Link PMC1326214 Disease Relevance 1.28 Pain Relevance 0

Furthermore, the ELISA detected higher levels of CA1 in the synovial fluid from patients with AS compared to the OA samples.

Gene_expression (detected) of CA1 in synovial fluid associated with spinal inflammation and osteoarthritis

18) Confidence 0.58 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 1.92 Pain Relevance 1.23

The current study detected increased expression of CA1 in the synovial tissue of AS patients.

Gene_expression (expression) of CA1 associated with spinal inflammation

19) Confidence 0.58 Published 2010 Journal BMC Musculoskelet Disord Section Body Doc Link PMC3012668 Disease Relevance 2.24 Pain Relevance 0.98

The proteomic approach revealed significantly increased expression of carbonic anhydrase I (CA1) in the synovial membrane of patients with AS as compared with the RA and OA tissue samples.

Gene_expression (expression) of carbonic anhydrase I associated with spinal inflammation, rheumatoid arthritis and osteoarthritis

20) Confidence 0.58 Published 2010 Journal BMC Musculoskelet Disord Section Abstract Doc Link PMC3012668 Disease Relevance 2.13 Pain Relevance 1.30

General Comments

This test has worked.