INT111477

Context Info Confidence 0.74 First Reported 2002 Last Reported 2010 Negated 22 Speculated 2 Reported most in Body Documents 90 Total Number 93 Disease Relevance 86.76 Pain Relevance 4.07

Golgi apparatus (KRT7) nucleolus (KRT7) structural molecule activity (KRT7) nucleus (KRT7) cytoskeleton organization (KRT7) cytoplasm (KRT7)

Anatomy Link Frequency thyroid 4 epithelial cells 3 stomach 2 smooth muscle 1 intestinal epithelium 1 KRT7 (Homo sapiens)

Pain Link Frequency Relevance Heat Inflammation 172 99.18 anesthesia 9 93.96 backache 20 93.92 imagery 66 93.04 Pain 35 92.96 Bile 82 92.44 Angina 2 90.28 Dysuria 1 85.28 Crohn's disease 2 83.76 palliative 27 82.08

Disease Link Frequency Relevance Heat Cancer 3189 100.00 Adenocarcinoma 1477 99.98 Mucinous Adenocarcinoma 11 99.98 Metastasis 647 99.96 Thyroid Neoplasm 10 99.90 Ependymoma 259 99.88 Carcinoma 1020 99.84 Neuroendocrine Cancer 124 99.84 Mesothelioma 102 99.72 Renal Cancer 332 99.70

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

What was interesting was the immunophenotype of the lesion: cytokeratin (CK) 20 expression was very focal in the adenocarcinoma component and negative in the neuroendocrine carcinoma, while CK 7 was expressed strongly in the adenocarcinoma and only focally in the neuroendocrine component.

Gene_expression (expressed) of CK 7 associated with adenocarcinoma and neuroendocrine cancer

1) Confidence 0.74 Published 2006 Journal Virchows Arch. Section Abstract Doc Link 16508780 Disease Relevance 1.32 Pain Relevance 0.08

Later, the immunohistochemical examination revealed that cytokeratin 7 (CK7) and thyroid transcription factor 1 (TTF-1) were distinctly detected but cytokeratin 20 (CK20) never expressed.

Gene_expression (detected) of CK7 in thyroid

2) Confidence 0.74 Published 2007 Journal World J Surg Oncol Section Body Doc Link PMC1821329 Disease Relevance 1.20 Pain Relevance 0.09

Later, the immunohistochemical examination revealed that cytokeratin 7 (CK7) and thyroid transcription factor 1 (TTF-1) were distinctly detected but cytokeratin 20 (CK20) never expressed.

Gene_expression (detected) of cytokeratin 7 in thyroid

3) Confidence 0.74 Published 2007 Journal World J Surg Oncol Section Body Doc Link PMC1821329 Disease Relevance 1.21 Pain Relevance 0.09

4 integrin subunit) and RM-derived cell lines (e.g. ezrin, bFGF, the tPA and uPA plasminogen activators, N-cadherin, cytokeratin 7), and others that were reduced in malignant cells (TRAIL, cytokeratin 19, ?

Gene_expression (ezrin) of cytokeratin 7 associated with malignant neoplastic disease

4) Confidence 0.65 Published 2007 Journal Biomarker Insights Section Body Doc Link PMC2717840 Disease Relevance 0.86 Pain Relevance 0.04

Classically, adenocarcinoma of the lung, breast ovary and omentum are CK7 positive and CK20 negative, while adenocarcinoma of colorectal origin tends to have the opposite (CK7 negative and CK20 positive) [4,15].

Neg (negative) Gene_expression (negative) of CK7 in ovary associated with adenocarcinoma

5) Confidence 0.65 Published 2007 Journal World J Surg Oncol Section Body Doc Link PMC2092434 Disease Relevance 0.85 Pain Relevance 0

Nor did the result for KRT7 (cytokeratin 7), which has been shown to be overexpressed in Chr [20].

Gene_expression (overexpressed) of cytokeratin 7 associated with carcinoma

6) Confidence 0.65 Published 2002 Journal BMC Bioinformatics Section Body Doc Link PMC130042 Disease Relevance 1.08 Pain Relevance 0

Recently, Chhieng et al. described that an adenocarcinoma was likely a primary lung tumor when it was of the CK7 positive/CK20 negative and TTF-1 positive phenotype [1].

Neg (negative) Gene_expression (positive) of CK7 in lung associated with adenocarcinoma and lung cancer

7) Confidence 0.64 Published 2007 Journal World J Surg Oncol Section Body Doc Link PMC1821329 Disease Relevance 1.23 Pain Relevance 0.08

Sections were stained routinely (H&E, trichrome, reticulin) as well as immunostained for detection of CK7, CK19, ?

Gene_expression (detection) of CK7

8) Confidence 0.64 Published 2003 Journal Comp Hepatol Section Body Doc Link PMC166154 Disease Relevance 0.40 Pain Relevance 0.09

The ileocecal lesion was diagnosed as a recurrence because of the histological findings, which included mucosal preservation, a similarity with the histologic type of stomach carcinoma, and atypical immunoreactivity for primary colon carcinoma; the lesion was negative for both cytokeratin 7 and cytokeratin 20.

Neg (negative) Gene_expression (negative) of cytokeratin 7 in colon associated with colon cancer, stomach cancer and recurrence

9) Confidence 0.60 Published 2004 Journal Acta Med. Okayama Section Abstract Doc Link 15551760 Disease Relevance 0.94 Pain Relevance 0.16

However, the cells were all negative for cytokeratin 7 and EMA.

Neg (negative) Gene_expression (negative) of cytokeratin 7

10) Confidence 0.57 Published 2008 Journal J Med Case Reports Section Body Doc Link PMC2430707 Disease Relevance 1.28 Pain Relevance 0

Nor did the result for KRT7 (cytokeratin 7), which has been shown to be overexpressed in Chr [20].

Gene_expression (overexpressed) of KRT7 associated with carcinoma

11) Confidence 0.56 Published 2002 Journal BMC Bioinformatics Section Body Doc Link PMC130042 Disease Relevance 1.08 Pain Relevance 0

The possibility of prostatic metastasis was considered and confirmed on immunohistochemical staining for PSA (Figure 2) and, given this, stains for CK7, CK20 and CDX2 immunoprofile were considered unnecessary.

Gene_expression (immunoprofile) of CK7 associated with metastasis

12) Confidence 0.51 Published 2007 Journal World J Surg Oncol Section Body Doc Link PMC1779271 Disease Relevance 0.83 Pain Relevance 0

The luminal cells were strongly immunoreactive with CK7 and with CD117 (Fig. 11).

Gene_expression (immunoreactive) of CK7

13) Confidence 0.44 Published 2008 Journal Head Neck Pathol Section Body Doc Link PMC2807538 Disease Relevance 0.41 Pain Relevance 0

All of the freshly isolated cells in the cytospin preparations expressed CK7.

Gene_expression (expressed) of CK7

14) Confidence 0.41 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2773740 Disease Relevance 0.07 Pain Relevance 0

In contrast, only about two thirds of the tarsal and bulbar cells expressed CK7 by flow cytometry.

Gene_expression (expressed) of CK7

15) Confidence 0.41 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2773740 Disease Relevance 0.07 Pain Relevance 0

An immunohistochemical panel showed the tissue to be cytokeratin 20 (CK-20) positive, cytokeratin 7 (CK-7) negative and villin negative, thus in keeping with a colorectal primary tumour.

Gene_expression (negative) of cytokeratin 7 associated with cancer

16) Confidence 0.39 Published 2006 Journal World J Surg Oncol Section Body Doc Link PMC1716166 Disease Relevance 1.53 Pain Relevance 0.10

An immunohistochemical panel showed the tissue to be cytokeratin 20 (CK-20) positive, cytokeratin 7 (CK-7) negative and villin negative, thus in keeping with a colorectal primary tumour.

Gene_expression (negative) of CK-7 associated with cancer

17) Confidence 0.39 Published 2006 Journal World J Surg Oncol Section Body Doc Link PMC1716166 Disease Relevance 1.52 Pain Relevance 0.10

For the detection of CK7, the slides were treated as described above but without antigen retrieval, and incubated overnight at 4°C with mouse anti-human CK7, clone OV-TL 12/30 (Dakocytomation), diluted 1:25 in PBS with 1% bovine serum albumin.

Gene_expression (detection) of CK7

18) Confidence 0.37 Published 2004 Journal Comp Hepatol Section Body Doc Link PMC535891 Disease Relevance 0 Pain Relevance 0

The supernatant was removed and cells were incubated with CK7-FITC antibody (10 ?

Gene_expression (incubated) of CK7

19) Confidence 0.36 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2773740 Disease Relevance 0.30 Pain Relevance 0

K8, K18, K19, and K7 are expressed in all cell layers.

Gene_expression (expressed) of K7

20) Confidence 0.35 Published 2008 Journal Histochem Cell Biol Section Body Doc Link PMC2386534 Disease Relevance 1.02 Pain Relevance 0

General Comments

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