INT113104

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Context Info
Confidence 0.65
First Reported 2002
Last Reported 2010
Negated 4
Speculated 0
Reported most in Body
Documents 18
Total Number 20
Disease Relevance 7.13
Pain Relevance 1.20

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
plasma 2
lymphocytes 1
HEL 1
upper 1
SCFV (Homo sapiens)
Pain Link Frequency Relevance Heat
rheumatoid arthritis 129 92.40 High High
Osteoarthritis 123 92.08 High High
Inflammatory response 6 89.16 High High
Arthritis 81 87.12 High High
cINOD 6 86.60 High High
Morphine 9 86.56 High High
Pain 1 86.44 High High
Crohn's disease 6 83.76 Quite High
Infliximab 9 78.48 Quite High
palliative 6 74.92 Quite High
Disease Link Frequency Relevance Heat
Cardiovascular Disorder Under Development 12 99.88 Very High Very High Very High
Togavirus Infection 14 99.64 Very High Very High Very High
Ovarian Cancer 191 99.36 Very High Very High Very High
Disease 114 98.52 Very High Very High Very High
Cancer 562 97.80 Very High Very High Very High
Sprains And Strains 104 97.72 Very High Very High Very High
Disease Progression 18 96.80 Very High Very High Very High
Adverse Drug Reaction 6 94.28 High High
Breast Cancer 24 92.76 High High
Rheumatoid Arthritis 133 92.40 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Affinity-matured mutations were introduced into scFv-streptavidin for functional studies.
Gene_expression (introduced) of scFv
1) Confidence 0.65 Published 2009 Journal J. Immunol. Section Abstract Doc Link 19812201 Disease Relevance 0.26 Pain Relevance 0.09
The antibodies were identified by screening high-density colonies of Escherichia coli expressing soluble scFv antibody fragments without prior expression on bacteriophage (phage display).
Gene_expression (expressing) of scFv
2) Confidence 0.46 Published 2003 Journal J. Immunol. Methods Section Abstract Doc Link 12972195 Disease Relevance 0 Pain Relevance 0.23
No scFv(FRP5)-ETA could be detected in patient plasma at the 2 ?
Gene_expression (detected) of scFv in plasma
3) Confidence 0.26 Published 2005 Journal Breast Cancer Res Section Body Doc Link PMC1242130 Disease Relevance 0.87 Pain Relevance 0.13
Recombinant scFv(FRP5)-ETA was produced as an experimental drug under Good Manufacturing Practice conditions and was kindly provided by Ciba Geigy AG (Basel, Switzerland).
Gene_expression (produced) of scFv
4) Confidence 0.26 Published 2005 Journal Breast Cancer Res Section Body Doc Link PMC1242130 Disease Relevance 0.20 Pain Relevance 0.07
The scFv genes derived from the anti-NIP B1.8 [18] and the anti-CEA MFE-23 [31] antibodies were similarly assembled and expressed as soluble secreted proteins in human HEK293 cells and purified from conditioned medium by immobilized metal affinity chromatography.
Gene_expression (expressed) of scFv
5) Confidence 0.24 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2670539 Disease Relevance 0 Pain Relevance 0
In these conditions, only the trimerbody approached saturation of the antigen surface, whereas the scFv bound slowly and showed more rapid apparent dissociation.
Gene_expression (bound) of scFv
6) Confidence 0.24 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2670539 Disease Relevance 0.12 Pain Relevance 0
Detection of scFv(FRP5)-ETA plasma levels by sandwich ELISA
Gene_expression (Detection) of scFv in plasma
7) Confidence 0.22 Published 2005 Journal Breast Cancer Res Section Body Doc Link PMC1242130 Disease Relevance 0.71 Pain Relevance 0
Complete or partial remissions were not observed after scFv(FRP5)-ETA treatment, which was not unexpected given the severity of the patients' disease and their tumor load.
Neg (not) Gene_expression (unexpected) of scFv associated with cancer and disease
8) Confidence 0.22 Published 2005 Journal Breast Cancer Res Section Body Doc Link PMC1242130 Disease Relevance 1.19 Pain Relevance 0.05
The mAb FRP5 and its scFv derivatives recognize a peptide epitope located in the N-terminal region of the receptor [14,31].
Gene_expression (derivatives) of scFv
9) Confidence 0.22 Published 2005 Journal Breast Cancer Res Section Body Doc Link PMC1242130 Disease Relevance 0.86 Pain Relevance 0.21
Only in five patients, however, could antibodies with neutralizing capacity against scFv(FRP5)-ETA be detected in cell viability assays.
Gene_expression (detected) of scFv
10) Confidence 0.20 Published 2005 Journal Breast Cancer Res Section Body Doc Link PMC1242130 Disease Relevance 0.11 Pain Relevance 0
To select for a specific scFv to hen egg lysozyme (HEL), which served as a control, we used a standard protocol.


Gene_expression (hen) of scFv in HEL
11) Confidence 0.13 Published 2010 Journal Arthritis and Rheumatism Section Body Doc Link PMC2905615 Disease Relevance 0.91 Pain Relevance 0.32
In some experiments the effluents were enriched for c-myc-expresser yeast with anti-c-myc magnetic beads (Milenyi) to remove yeast that failed to bind to the control column because they lacked scFv expression.
Neg (lacked) Gene_expression (expression) of scFv
12) Confidence 0.12 Published 2008 Journal J Transl Med Section Body Doc Link PMC2503970 Disease Relevance 0 Pain Relevance 0
Using a blocking solution of 0.5% bovine serum albumin (BSA) in phosphate buffered saline (PBS), sections were incubated overnight at 4°C with either the selected scFv (10 ?
Gene_expression (selected) of scFv
13) Confidence 0.11 Published 2010 Journal Arthritis and Rheumatism Section Body Doc Link PMC2905615 Disease Relevance 0.17 Pain Relevance 0.06
When control mTNFRII-Fc (lacking the scFv, and thus with a molecular weight ?
Neg (lacking) Gene_expression (lacking) of scFv
14) Confidence 0.11 Published 2010 Journal Arthritis and Rheumatism Section Body Doc Link PMC2905615 Disease Relevance 0.26 Pain Relevance 0.04
The recognition sequences of the yeast-display scFv that demonstrated a preferential binding to case- over control-pool serum were amplified by PCR and cloned in yeast by gap repair using co-transformation with pTOR2 vector [1] for production of tagged, yeast-secreted scFv.
Gene_expression (production) of scFv
15) Confidence 0.08 Published 2008 Journal J Transl Med Section Body Doc Link PMC2503970 Disease Relevance 0 Pain Relevance 0
Generation of large scFv libraries from ovarian cancer patient lymphocytes could improve the discovery rate using this method.
Gene_expression (Generation) of scFv in lymphocytes associated with ovarian cancer
16) Confidence 0.08 Published 2008 Journal J Transl Med Section Body Doc Link PMC2503970 Disease Relevance 0.31 Pain Relevance 0
A yeast-display scFv library [2] was first enriched for protein serum-specific scFv with biotinylated case-pool serum by magnetic and flow sortings (fig. 1A1–2), until more than 95% of the selected yeast expressed scFv that bound to biotinylated case-pool serum (fig 2A,C, upper right quadrants).
Gene_expression (expressed) of scFv in upper
17) Confidence 0.08 Published 2008 Journal J Transl Med Section Body Doc Link PMC2503970 Disease Relevance 0.06 Pain Relevance 0
Immobilization of albumin-depleted control-pool serum on a plastic plate for panning gave poor results, and depletion experiments using biotinylated albumin-depleted control-pool serum for fluorescent cytometry sorting biased the selection towards yeast that did not express scFv (non-expresser yeast) (data not shown).
Neg (not) Gene_expression (express) of scFv
18) Confidence 0.08 Published 2008 Journal J Transl Med Section Body Doc Link PMC2503970 Disease Relevance 0 Pain Relevance 0
ScFv was expressed in Escherichia coli and preliminary experiments with dot blot analyses show the binding of ScFv to the CS peptide repeat.
Gene_expression (expressed) of ScFv
19) Confidence 0.07 Published 2002 Journal J Insect Sci Section Body Doc Link PMC405832 Disease Relevance 0.56 Pain Relevance 0
Production of higher amounts of ScFv using a Sindbis virus expression system is underway.
Gene_expression (Production) of ScFv associated with togavirus infection
20) Confidence 0.06 Published 2002 Journal J Insect Sci Section Body Doc Link PMC405832 Disease Relevance 0.54 Pain Relevance 0

General Comments

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