INT114138

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Context Info
Confidence 0.59
First Reported 2004
Last Reported 2007
Negated 1
Speculated 0
Reported most in Body
Documents 18
Total Number 19
Disease Relevance 7.07
Pain Relevance 0.53

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

transport (Vdac1) mitochondrion (Vdac1) plasma membrane (Vdac1)
protein complex (Vdac1) transmembrane transport (Vdac1)
Anatomy Link Frequency
neuronal 6
embryonic kidney 1
Vdac1 (Mus musculus)
Pain Link Frequency Relevance Heat
gABA 9 96.96 Very High Very High Very High
anesthesia 5 85.72 High High
ischemia 12 46.64 Quite Low
addiction 58 5.00 Very Low Very Low Very Low
fluoxetine 34 5.00 Very Low Very Low Very Low
fibrosis 10 5.00 Very Low Very Low Very Low
adenocard 6 5.00 Very Low Very Low Very Low
Bile 6 5.00 Very Low Very Low Very Low
Inflammation 4 5.00 Very Low Very Low Very Low
agonist 2 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Neurodegenerative Disease 80 99.92 Very High Very High Very High
Apoptosis 242 99.64 Very High Very High Very High
Targeted Disruption 20 99.34 Very High Very High Very High
Death 178 98.86 Very High Very High Very High
Disease 82 94.72 High High
Stress 32 72.04 Quite High
Neuroblastoma 16 71.20 Quite High
Stroke 16 66.76 Quite High
Hypoxia 16 53.64 Quite High
Pressure And Volume Under Development 54 48.92 Quite Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The absence of VDAC-1 or VDAC-3 had no effect on the ability of neuroactive steroids to modulate GABA(A) receptor function as evidenced by radioligand ([(35)S] t-butylbicyclophosphorothionate) binding or by electrophysiological studies.
Neg (absence) Gene_expression (absence) of VDAC-1 associated with gaba
1) Confidence 0.59 Published 2004 Journal J. Pharmacol. Exp. Ther. Section Abstract Doc Link 14593090 Disease Relevance 0 Pain Relevance 0.53
This signal peptide is eventually cleaved away to produce a plasmalemmal VDAC protein identical to the mitochondrial one.
Gene_expression (produce) of plasmalemmal VDAC protein
2) Confidence 0.23 Published 2005 Journal Purinergic Signal Section Body Doc Link PMC2096548 Disease Relevance 0.11 Pain Relevance 0
Moreover, consistent with our hypothesis of maxi-anion channelmediated ATP release, ATP release was diminished in VDAC-1 knock-out mice and augmented in cells overexpressing pl-VDAC-1 [275].
Gene_expression (overexpressing) of VDAC-1 associated with targeted disruption
3) Confidence 0.21 Published 2005 Journal Purinergic Signal Section Body Doc Link PMC2096548 Disease Relevance 0.10 Pain Relevance 0
Our results suggest a previously unknown mechanistic basis linking transcriptional dysregulation to cell death in neurodegeneration through TBP mediated alteration of Vdac1 expression.
Gene_expression (expression) of Vdac1 associated with death
4) Confidence 0.11 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.56 Pain Relevance 0
Vdac1 overexpression, independently implicated in triggering cell death was one of the effects of polyglutamine expansion in TBP, identified in our study.
Gene_expression (overexpression) of Vdac1 associated with death
5) Confidence 0.11 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.72 Pain Relevance 0
Further, we studied cytochrome c release from the mitochondria following increase in VDAC1 expression.
Gene_expression (expression) of VDAC1
6) Confidence 0.11 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.46 Pain Relevance 0
The cells expressing 59Q TBP showed a gradual increase in cytochrome c release resulting in substantially higher Cyt c levels at time points beyond 36 hours compared to cells which express the vector alone (Fig. 7B), in agreement with increased expression of VDAC1.
Gene_expression (expression) of VDAC1
7) Confidence 0.11 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.57 Pain Relevance 0
A change in the expression level of Vdac1 has recently been shown to be a critical determinant of cell death in embryonic kidney cells [18].
Gene_expression (expression) of Vdac1 in embryonic kidney associated with death
8) Confidence 0.10 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.28 Pain Relevance 0
Consistent with cytochrome c release, flow cytometric analysis of AnnexinV-Phycoerythrin (AnnexinV-PE) positive cells revealed that VDAC1 overexpression induced apoptosis (Fig. 8G).


Gene_expression (overexpression) of VDAC1 associated with apoptosis
9) Confidence 0.09 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.50 Pain Relevance 0
In our studies, VDAC1 overexpression lead to elevated cytochrome c release and apoptotic cell death in mouse neuronal cells.
Gene_expression (overexpression) of VDAC1 in neuronal associated with apoptosis and death
10) Confidence 0.09 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.59 Pain Relevance 0
Cytochrome c release was three fold higher in VDAC1 overexpressing cells (Fig. 8F).
Gene_expression (overexpressing) of VDAC1
11) Confidence 0.09 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.52 Pain Relevance 0
Subsequently, radioactively labeled probe prepared from purified PCR products of Vdac1, Dctn5, Ubb and Gfp using NEBlotTM kit (NEB) according to manufacturer instruction.
Gene_expression (products) of Vdac1
12) Confidence 0.09 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0 Pain Relevance 0
Expression of mVdac1GFP in transfected N2a cells was confirmed by reverse transcription followed by PCR (RT-PCR).
Gene_expression (Expression) of mVdac1GFP
13) Confidence 0.09 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.07 Pain Relevance 0
Survival related gene, Voltage dependent anion channel (Vdac1) and ubiquitin related gene, ubiquitin B (Ubb) and genes involved in localized neuronal translation, cytoplasmic beta-actin (Actb), eukaryotic elongation factor2 (Eef2) and eukaryotic elongation factor 1 (Eef1alpha 1); retrograde transport, p25 subunit of dynactin (Dctn5), were amongst the genes that showed significant upregulation in 59Q transfected cells as compared to internally normalized expression levels in vector transfected controls (Fig. 1).
Gene_expression (expression) of Vdac1 in neuronal
14) Confidence 0.09 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.38 Pain Relevance 0
We independently confirmed that Vdac1 overexpression in mouse neuronal cells results in higher cytochrome c release and apoptosis, as has been reported for non-neuronal cells previously [23].
Gene_expression (overexpression) of Vdac1 in neuronal associated with apoptosis
15) Confidence 0.09 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.40 Pain Relevance 0
Previous studies have shown that overexpression of VDAC1 can lead to apoptosis in non-neuronal cells.
Gene_expression (overexpression) of VDAC1 in neuronal associated with apoptosis
16) Confidence 0.09 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.65 Pain Relevance 0
We confirmed a similar effect of VDAC1 in neuronal cells by cloning and overexpressing mouse VDAC1 (Fig. 8A–E).
Gene_expression (overexpressing) of VDAC1 in neuronal
17) Confidence 0.09 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.53 Pain Relevance 0
In this study we show that alleles of TBP harbouring abnormally expanded polyQ tracts not only form intranuclear aggregates in cultured mouse neuronal cells, but also result in overexpression of the voltage dependent anion channel, Vdac1 at both the RNA and protein level.
Gene_expression (overexpression) of Vdac1 in neuronal
18) Confidence 0.09 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.57 Pain Relevance 0
Similar method was followed for Cyt c release assay in case of mVdac1GFP transfected cells.
Gene_expression (transfected) of mVdac1GFP
19) Confidence 0.09 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2064964 Disease Relevance 0.07 Pain Relevance 0

General Comments

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