INT114201

From wiki-pain
Jump to: navigation, search
Context Info
Confidence 0.78
First Reported 2003
Last Reported 2010
Negated 2
Speculated 0
Reported most in Body
Documents 8
Total Number 24
Disease Relevance 14.20
Pain Relevance 7.21

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

transport (Slc32a1)
Anatomy Link Frequency
brains 6
neurons 4
plasma 1
spinal cord 1
forebrains 1
Slc32a1 (Mus musculus)
Pain Link Frequency Relevance Heat
gABA 714 100.00 Very High Very High Very High
GABAergic 415 100.00 Very High Very High Very High
amygdala 40 99.40 Very High Very High Very High
Spinal cord 279 98.64 Very High Very High Very High
Morphine 8 98.32 Very High Very High Very High
Pain 18 97.52 Very High Very High Very High
Neurotransmitter 131 95.84 Very High Very High Very High
withdrawal 3 95.08 Very High Very High Very High
Hippocampus 50 91.44 High High
Pyramidal cell 16 89.96 High High
Disease Link Frequency Relevance Heat
Targeted Disruption 647 99.96 Very High Very High Very High
Cleft Palate 272 99.40 Very High Very High Very High
Apoptosis 34 99.12 Very High Very High Very High
Respiratory Failure 51 98.64 Very High Very High Very High
Pain 18 97.52 Very High Very High Very High
Umbilical Hernia 408 96.88 Very High Very High Very High
Anxiety Disorder 56 96.24 Very High Very High Very High
Convulsion 17 95.52 Very High Very High Very High
Substance Withdrawal Syndrome 3 95.08 Very High Very High Very High
Neurodegenerative Disease 39 94.88 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Western blot showed that the level of VGAT protein expression in VGATfloxneo/floxneo brain was not different from the wild-type brain (Additional file 1: Supplementary Figure S1).
Gene_expression (expression) of VGAT protein in brain
1) Confidence 0.78 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 0.72 Pain Relevance 0.06
Western blot analysis revealed no VGAT protein expression in embryonic day (E) 18.5 VGAT-/- brain, whereas VGAT protein expression in VGAT+/- mouse brains was reduced to about half of the wild-type level (Figure 1D).
Gene_expression (expression) of VGAT protein in brains
2) Confidence 0.78 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 0.58 Pain Relevance 0.03
These results suggest that a loxP sequence and an frt-flanked phosphoglycerate kinase promoter-driven neomycin-resistance gene (PGK-Neo) inserted into intron 1 and the 3'-flanking region of the VGAT gene, respectively, do not affect the expression level of VGAT protein.
Gene_expression (expression) of VGAT protein
3) Confidence 0.78 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 0.64 Pain Relevance 0.20
Western blot analysis revealed no VGAT protein expression in embryonic day (E) 18.5 VGAT-/- brain, whereas VGAT protein expression in VGAT+/- mouse brains was reduced to about half of the wild-type level (Figure 1D).
Neg (no) Gene_expression (expression) of VGAT protein in brains
4) Confidence 0.78 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 0.57 Pain Relevance 0.03
VGAT is present in both GABAergic and glycinergic neurons and is also called the vesicular inhibitory amino acid transporter (VIAAT) [3,9].
Gene_expression (present) of VGAT in neurons associated with gabaergic
5) Confidence 0.68 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 1.09 Pain Relevance 0.70
Generation of VGAT-/- mice
Gene_expression (Generation) of VGAT
6) Confidence 0.68 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 0.71 Pain Relevance 0.18
Our Western blot analysis showed that the expression levels of both GAD65 and GAD67 in VGAT+/+ and VGAT-/- brains were similar (Figure 2B).
Gene_expression (expression) of VGAT in brains
7) Confidence 0.68 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 0.21 Pain Relevance 0.62
Phenotypes of VGAT and GAD67 knockouts outside of the brain
Gene_expression (Phenotypes) of VGAT in brain
8) Confidence 0.67 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 1.07 Pain Relevance 0.09
As before, we confirmed the purity of the selected GABAergic and glutamatergic neuron samples using qRT-PCR analysis of VGLUT1/VGAT expression (Table 1).
Gene_expression (expression) of VGAT in glutamatergic neuron associated with gabaergic
9) Confidence 0.65 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0.06 Pain Relevance 0.35
Western blot analysis revealed no VGAT protein expression in embryonic day (E) 18.5 VGAT-/- brain, whereas VGAT protein expression in VGAT+/- mouse brains was reduced to about half of the wild-type level (Figure 1D).
Gene_expression (expression) of VGAT in brains
10) Confidence 0.59 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 0.58 Pain Relevance 0.03
Elevations in GABA and glycine contents in VGAT-/- forebrains
Gene_expression (contents) of VGAT in forebrains associated with gaba
11) Confidence 0.59 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 0.51 Pain Relevance 0.30
VGAT is present in both GABAergic and glycinergic neurons and is also called the vesicular inhibitory amino acid transporter (VIAAT) [3,9].
Gene_expression (called) of vesicular inhibitory amino acid transporter in neurons associated with gabaergic
12) Confidence 0.59 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 1.10 Pain Relevance 0.72
Therefore, the VGAT-floxneo allele allowed for Cre-mediated conditional inactivation of the VGAT gene, and mice carrying these alleles will be useful in examining VGAT function at different developmental stages and in distinct cell types.


Gene_expression (allele) of VGAT-floxneo in developmental stages
13) Confidence 0.59 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 0.62 Pain Relevance 0.21
Here, we generated floxed VGAT knockout mice, in which exons 2 and 3 of the VGAT gene were flanked by loxP sites.
Gene_expression (flanked) of VGAT gene associated with targeted disruption
14) Confidence 0.59 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 1.11 Pain Relevance 0.10
VGAT is present in both GABAergic and glycinergic neurons and is also called the vesicular inhibitory amino acid transporter (VIAAT) [3,9].
Gene_expression (called) of VGAT in neurons associated with gabaergic
15) Confidence 0.59 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 1.11 Pain Relevance 0.73
Our results from the electrophysiological recordings of spinal cord MNs indicate that the inhibitory synaptic transmission was clearly absent in the VGAT knockout MNs, but that the excitatory synaptic transmission was present.
Neg (absent) Gene_expression (absent) of VGAT in spinal cord associated with targeted disruption and spinal cord
16) Confidence 0.59 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 0.10 Pain Relevance 0.49
Our results, summarized in Table 1, demonstrate that a relatively high level of VGAT transcripts was detected in the absence of a VGLUT1 signal in GFPpos samples, and conversely, a similarly high level of expression of VGLUT1 was determined for GFPneg cells in which VGAT RNA was undetectable.
Gene_expression (detected) of VGAT
17) Confidence 0.58 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0.08 Pain Relevance 0.13
The targeting strategy used for the generation of VGAT knockout mice is shown in Figure 1A.
Gene_expression (generation) of VGAT associated with targeted disruption
18) Confidence 0.52 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 0.70 Pain Relevance 0.15
Generation of VGAT knockout mice
Gene_expression (Generation) of VGAT associated with targeted disruption
19) Confidence 0.52 Published 2010 Journal Mol Brain Section Body Doc Link PMC3023674 Disease Relevance 1.19 Pain Relevance 0.12
We generated GABA transporter I (GAT1)-overexpressing mice to investigate whether the GABAergic system and GABA transporter are involved in morphine-induced reward effects and withdrawal symptoms.
Gene_expression (overexpressing) of GABA transporter associated with gaba, gabaergic, withdrawal and morphine
20) Confidence 0.37 Published 2003 Journal J. Neurosci. Res. Section Abstract Doc Link 14598306 Disease Relevance 0.38 Pain Relevance 0.96

General Comments

This test has worked.

Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox