INT114998

From wiki-pain
Jump to: navigation, search
Context Info
Confidence 0.53
First Reported 2003
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 33
Total Number 35
Disease Relevance 2.65
Pain Relevance 8.93

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

transmembrane transport (Kcna6)
Anatomy Link Frequency
neurons 13
SH-SY5Y 1
adult brain 1
soma 1
neurite 1
Kcna6 (Rattus norvegicus)
Pain Link Frequency Relevance Heat
Nav1.2 724 99.88 Very High Very High Very High
Central nervous system 47 99.62 Very High Very High Very High
imagery 224 99.56 Very High Very High Very High
Action potential 162 99.08 Very High Very High Very High
sodium channel 536 99.06 Very High Very High Very High
Nav1.7 3 98.80 Very High Very High Very High
Nerve growth factor 3 97.76 Very High Very High Very High
potassium channel 66 97.12 Very High Very High Very High
Neuronal excitability 3 96.64 Very High Very High Very High
ischemia 32 94.20 High High
Disease Link Frequency Relevance Heat
Generalized Anxiety Disorder 180 100.00 Very High Very High Very High
Middle Cerebral Artery Infarction 3 98.72 Very High Very High Very High
Alzheimer's Dementia 12 98.20 Very High Very High Very High
Drug Induced Neurotoxicity 3 96.88 Very High Very High Very High
Nervous System Injury 1 95.36 Very High Very High Very High
Cv Unclassified Under Development 29 94.20 High High
Hypoxia 38 94.12 High High
Neuroblastoma 5 93.00 High High
Disease 51 90.72 High High
Apoptosis 523 90.08 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
By the 14 DIV time point, approximately 87% of the cells showed dense Kv2.1 expression in one or two AIS domains along with expression over the soma.
Gene_expression (expression) of Kv2 in soma
1) Confidence 0.53 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0
The data presented thus far indicate that both the endogenous and exogenously expressed Kv2.1 accumulate at the AIS in hippocampal cultures, often preferring this region to other cell surface sites early in expression.
Gene_expression (expressed) of Kv2
2) Confidence 0.53 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0
Our initial imaging experiments involved expressing GFP and HA epitope-tagged Kv2.1 (EGFP-Kv2.1-HA) in rat hippocampal neurons grown in culture for 7–9 days.
Gene_expression (expressing) of Kv2 in neurons associated with imagery
3) Confidence 0.53 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0.10
The detection of MAP2, ankyrinG, GAD and Kv2.1 in cultured hippocampal neurons has been performed by multiple groups using either the same antibodies as used here or closely related ones [2,7,18,20,30-36].
Gene_expression (detection) of Kv2 in neurons associated with generalized anxiety disorder
4) Confidence 0.53 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0.15 Pain Relevance 0
Previous Kv2.1 localizations in adult brain, as performed by other investigators [3,8] and ourselves (unpublished results), also failed to detect Kv2.1 expression in the AIS.
Gene_expression (expression) of Kv2 in adult brain
5) Confidence 0.53 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0.07 Pain Relevance 0.14
We observed Kv2.1 expression on MAP2-positive neurites in only 10% of the 7 DIV neurons and 2% of the day 10 and 14 cells.
Gene_expression (expression) of Kv2 in neurons
6) Confidence 0.46 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0.06
In contrast to the expected soma and proximal neurite expression [7,18], 85% of neurons expressing EGFP-Kv2.1-HA exhibited surface accumulation at only one of the proximal neurites at 6 h post transfection (see arrows in Fig. 1, 6 h).
Gene_expression (expressing) of Kv2 in neurite
7) Confidence 0.46 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0.08
The AIS localized mRFP/PAGFP-Kv2.1 was selected for photoactivation under RFP optics (rectangle, Fig. 5) and successive imaging was performed every two minutes thereafter.
Gene_expression (selected) of Kv2 in optics associated with imagery
8) Confidence 0.46 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0.17 Pain Relevance 0.15
In neurons expressing Kv2.1 for 18 h, surface clusters within the soma (arrowheads) became more apparent relative to that observed at 6 h postransfection (Fig. 1, 18 h).
Gene_expression (expressing) of Kv2 in neurons
9) Confidence 0.46 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0.07
We did observe variability in the endogenous Kv2.1 expression pattern, for 12% of 14 DIV neurons showed no AIS localization.
Gene_expression (expression) of Kv2 in neurons
10) Confidence 0.46 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0.13 Pain Relevance 0.07
Given that the Kv2.1 surface clusters are sites where trafficking vesicles deliver channel to the cell surface [14], it is possible that vesicles containing newly synthesized Kv2.1 are delivered first to the AIS.
Gene_expression (synthesized) of Kv2 in vesicles
11) Confidence 0.46 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0.03
Our initial imaging experiments involved expressing GFP and HA epitope-tagged Kv2.1 (EGFP-Kv2.1-HA) in rat hippocampal neurons grown in culture for 7–9 days.
Gene_expression (expressing) of Kv2 in neurons associated with imagery
12) Confidence 0.46 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0.10
This targeting of Kv2.1 to a single proximal neurite was observed with little or no clustering on the cell body.
Gene_expression (This) of Kv2 in proximal
13) Confidence 0.46 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0.07
As originally noted by Trimmer and colleagues [1], the Kv2.1 delayed rectifier is expressed primarily in the somatic region of hippocampal neurons where it is found in cell surface clusters that can co-localize with ryanodine receptors and SR-like subsurface cisterns [2,3].
Gene_expression (expressed) of Kv2 in neurons
14) Confidence 0.41 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0.03
While the expression of Kv2.1 within the axon initial segment (AIS) of cultured hippocampal neurons has previously been referred to as a tissue culture artifact [8], AIS localization was often the only cell surface expression observed in an individual cell.
Gene_expression (expression) of Kv2 in neurons
15) Confidence 0.41 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0.11 Pain Relevance 0
Exogenously expressed Kv2.1 targets to the axon initial segment in cultured hippocampal neurons before accumulating on the soma
Gene_expression (expressed) of Kv2 in neurons
16) Confidence 0.41 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0.09
It is highly unlikely that these distinct anti-Kv2.1 antibodies both nonspecifically bind AIS structures, especially since GFP-Kv2.1-HA also targets to this region in transfected neurons.


Gene_expression (unlikely) of GFP-Kv2 in neurons
17) Confidence 0.40 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0
In brief, EGFP was placed onto the Kv2.1 N-terminus and two HA epitopes were inserted in the extracellular loop between S1 and S2 transmembrane domains.


Gene_expression (placed) of Kv2
18) Confidence 0.40 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0 Pain Relevance 0.11
Similar regulation is observed in Kv2.1 transfected HEK cells [9].
Gene_expression (transfected) of Kv2
19) Confidence 0.40 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2592246 Disease Relevance 0.24 Pain Relevance 0.14
Using immunocytochemistry and Western blots, we detected consistently the expression of Kv2.1, and 4.3.
Gene_expression (expression) of Kv2
20) Confidence 0.38 Published 2008 Journal PLoS ONE Section Abstract Doc Link PMC2225502 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.

Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox