INT11624
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
MOR expression is regulated at the transcriptional and post-transcriptional levels. | |||||||||||||||
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These results indicate that re-initiation in MOR gene expression could play an important role in OPRM1 regulation. | |||||||||||||||
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However, the protein expression of the MOR gene is relatively low and the translational control of MOR gene has not been well studied. | |||||||||||||||
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The data suggest that MOP expression is controlled in part by initiation of the long form of MOP at the alternate site. | |||||||||||||||
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As the highest relative expression levels are in transformed neuronal cell lines, MOR1K expression is likely to be suppressed in native cellular conditions. | |||||||||||||||
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MOR1K expression was not observed in the connective tissue surrounding DRG or in peripheral leukocytes. | |||||||||||||||
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To test this hypothesis, we first characterized tissue-specific expression levels of MOR1K, its cellular localization, and agonist binding capacity to confirm potential functionality of this new receptor isoform. | |||||||||||||||
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Additionally, MOR1K was expressed at high levels in the human neuroblastoma cell lines Be2C and SH-SY5Y, while no expression was observed in monkey kidney COS-1 and human astrocytoma H4 cell lines. | |||||||||||||||
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Overexpression of MOR1K in mammalian cells revealed that this 6TM receptor is not expressed at the cell membrane, but instead is retained in the intracellular compartment (Fig.1C). | |||||||||||||||
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For the cAMP accumulation assay, MOR1 or MOR1K expressants were plated on 12 well plates and grown to 90% confluency. | |||||||||||||||
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These brain regions are known to also express MOR1 and contribute to the pharmacological effects of MOR agonists. | |||||||||||||||
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Genetic analyses revealed that allelic variants coding for higher MOR1K expression are associated with greater sensitivity to noxious stimuli and blunted responses to morphine[32]. | |||||||||||||||
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Results from RT-PCR revealed that MOR1K is expressed in the frontal lobe, medulla oblongata, insula, nucleus accumbens, pons, spinal cord, and dorsal root ganglion (DRG) (Fig.1A-B). | |||||||||||||||
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A moderate increase in morphine-evoked Ca2+ levels was also observed in Be2C cells transfected with MOR1 or empty vector, however this was likely due to the high endogenous expression of MOR1K in this cell line (Fig.1B). | |||||||||||||||
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For the cAMP accumulation assay, MOR1 or MOR1K expressants were plated on 12 well plates and grown to 90% confluency. | |||||||||||||||
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For MOR1 expressing cells, maximum NO production (7.5 pM) occurred at ~50 sec after administration of 1 ? | |||||||||||||||
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A moderate increase in morphine-evoked Ca2+ levels was also observed in Be2C cells transfected with MOR1 or empty vector, however this was likely due to the high endogenous expression of MOR1K in this cell line (Fig.1B). | |||||||||||||||
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First, we characterized the relative expression of MOR1K in human brain and spinal cord as well as peripheral leukocytes using real-time PCR (RT-PCR). | |||||||||||||||
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These data suggest that human MOR1K expression is restricted to neuronal cells. | |||||||||||||||
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Taken together, these findings suggest that activation of constitutively expressed MOR inhibits microglial cell chemotaxis and support the notion of an anti-inflammatory role of MOR within the brain. | |||||||||||||||
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