INT116830

Context Info Confidence 0.45 First Reported 2004 Last Reported 2010 Negated 1 Speculated 0 Reported most in Body Documents 10 Total Number 10 Disease Relevance 0.29 Pain Relevance 0.78

nucleoplasm (Itpr3) transport (Itpr3) endoplasmic reticulum (Itpr3) nucleolus (Itpr3) nucleus (Itpr3) transmembrane transport (Itpr3)

Anatomy Link Frequency tails 1 taste buds 1 mast cells 1 Itpr3 (Mus musculus)

Pain Link Frequency Relevance Heat Calcium channel 3 99.36 Potency 5 98.48 tail-flick 1 93.68 anesthesia 9 90.56 Kinase C 18 86.64 agonist 23 80.12 potassium channel 1 75.60 Hippocampus 10 51.52 antagonist 3 50.64 Central nervous system 14 27.36

Disease Link Frequency Relevance Heat Diabetes Mellitus 56 84.88 Adenoma 6 81.92 Thrombosis 30 50.00 Dementia 15 50.00 American Trypanosomiasis 4 50.00 Adhesions 17 44.00 Brain Hemorrhage 16 19.12 Hyperinsulinism 4 18.08 Obesity 2 14.52 Infarction 5 13.72

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Further investigation using mice lacking Lrmp/Jaw1 would provide insights regarding the role of Lrmp/Jaw1–IP3R3 interaction in taste tissue in vivo.

IP3R3 Binding (interaction) of

1) Confidence 0.45 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0.17 Pain Relevance 0

The hypothesis was tested that drugs that bind RyR or IP3 channels would affect the anesthetic potency of bupivacaine.

IP3 Binding (bind) of associated with potency

2) Confidence 0.42 Published 2004 Journal Eur J Pain Section Abstract Doc Link 14987625 Disease Relevance 0 Pain Relevance 0.47

Furthermore, we showed that Lrmp/Jaw1 may engage in direct protein–protein interaction with IP3R3.

IP3R3 Binding (interaction) of

3) Confidence 0.35 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

To investigate the protein–protein interaction between Lrmp/Jaw1 and IP3R3, we utilized the COS7 heterologous expression system and the FLAG epitope–tagged Lrmp/Jaw1 construct with/without a coiled-coil domain (FLAG-Lrmp/Jaw1 and FLAG-Lrmp/Jaw1?

IP3R3 Binding (interaction) of

4) Confidence 0.35 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

Therefore, the deletion of the coiled-coil domain did not affect subcellular localization of Lrmp/Jaw1 and IP3R3 but altered its interaction with IP3R3.

IP3R3 Neg (not) Binding (interaction) of

5) Confidence 0.33 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

Because IP3R3 was coexpressed with Trpm5 in taste buds (Pérez et al. 2002), we predicted that Lrmp/Jaw1 may colocalize and interact with IP3R3.

IP3R3 Binding (interact) of in taste buds

6) Confidence 0.33 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

However, this is difficult to justify, at least for IP3R3 trafficking, because deletion of the potential binding domain (coiled-coil domain of Lrmp/Jaw1) did not alter IP3R3 expression on the ER membrane in the COS7 heterologous expression system.

IP3R3 Binding (trafficking) of

7) Confidence 0.30 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0.12 Pain Relevance 0

However, mast cells lacking the major SOC channel Orai1 (22) show normal store content, suggesting a SOC channel–independent role of STIM1 in store refill, possibly through interaction with the IP3 receptors or SERCA pumps in the SR/ER.

IP3 Binding (interaction) of in mast cells

8) Confidence 0.04 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2442636 Disease Relevance 0 Pain Relevance 0.07

IP3 then binds to ‘receptor proteins’ in the membrane of the ER.

IP3 Binding (binds) of

9) Confidence 0.01 Published 2010 Journal The Open Biochemistry Journal Section Body Doc Link PMC2864432 Disease Relevance 0 Pain Relevance 0.19

interacts with the cytosolic tails of IP3 receptor [57] and TRPV5 calcium channel [58], thus providing further evidence of its cytosolic localization.

IP3 receptor Binding (interacts) of in tails associated with calcium channel

10) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0.05

General Comments

This test has worked.