INT116856
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
These in vivo experimental results are consistent with our previous results using an adenoviral vector expressing TRAIL protein. | |||||||||||||||
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Analysis of the purified rTRAIL protein demonstrated that both trimeric and dimeric TRAIL were present at a ratio of 4:1 [13] (data not shown). | |||||||||||||||
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It appears as if the apoptotic effect of the over-expression of TRAIL on the synovial fibroblasts from RA patients is mediated predominantly by TRAIL R1 as its expression varied from patient to patient and the extent of apoptosis following TRAIL gene transfer seemed to correlate with its expression intensity (data not shown). | |||||||||||||||
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It is possible that the proliferating rabbit synovium in vivo expresses higher levels of TRAIL receptors. | |||||||||||||||
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Although these previous results suggest that gene transfer of TRAIL could be therapeutic to treat certain pathologies associated with RA, there currently is no appropriate gene transfer system that could be used clinically to deliver TRAIL intra-articularly. | |||||||||||||||
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It appears as if the apoptotic effect of the over-expression of TRAIL on the synovial fibroblasts from RA patients is mediated predominantly by TRAIL R1 as its expression varied from patient to patient and the extent of apoptosis following TRAIL gene transfer seemed to correlate with its expression intensity (data not shown). | |||||||||||||||
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We did not observe any consistent pattern of expression of the TRAIL decoy receptors in the limited number of human synovial fibroblast cultures examined, suggesting that susceptibility to TRAIL is conferred through the level of expression of TRAIL R1 (data not shown). | |||||||||||||||
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The extent of apoptosis observed was similar to that observed following adenoviral gene transfer of TRAIL and FasL.
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In this study, we investigated the association between the expression of TRAIL and AS. | |||||||||||||||
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The results indicated that the expression levels of TRAIL mRNA, and serum sTRAIL were significantly elevated in AS patients, compared with RA patients and healthy controls, and there was a close association between TRAIL mRNA and sTRAIL levels. | |||||||||||||||
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In conclusion, upregulated expression of TRAIL might be somewhat specific for evaluation of AS. | |||||||||||||||
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OBJECTIVE: The present study investigated, through immunohistochemistry, the regional expression of TRAIL and in temporomandibular joint (TMJ) disc of anterior disc displacement with reduction (ADDwR) and without reduction (ADDwoR) patients, to help determine the relationship between TMJ disc displacement and apoptosis. | |||||||||||||||
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CONCLUSIONS: TRAIL and DR5 are overexpressed in displaced human TMJ disc, especially in the posterior disc attachment. | |||||||||||||||
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A human TRAIL cDNA fragment (corresponding to amino acids 114 to 281) obtained by PCR was cloned into the pET-23d (Novagen, Madison, WI, USA) plasmid, and expressed protein was purified using the His-band Resin and Buffer Kit (Novagen) [13]. | |||||||||||||||
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We did not observe any consistent pattern of expression of the TRAIL decoy receptors in the limited number of human synovial fibroblast cultures examined, suggesting that susceptibility to TRAIL is conferred through the level of expression of TRAIL R1 (data not shown). | |||||||||||||||
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It is possible that TRAIL reduces the leukocytic infiltrate through the direct induction of apoptosis of intra-articular leukocytes [21]. | |||||||||||||||
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Our previous studies with adenoviral gene transfer of membrane bound TRAIL, however, showed that it too was relatively inefficient in inducing synovial apoptosis, requiring a high multiplicity of infection to confer apoptosis in culture. | |||||||||||||||
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Cell sensitivity to TRAIL can be affected by TRAIL-receptor expression at the cell membrane, DR4/DR5 ratio and functionality of TRAIL-receptors. | |||||||||||||||
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Isolated disk cells were analyzed by flow cytometry for Fas, FasL, TRAIL, DR4 and DR5 expression. | |||||||||||||||
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Expression of TRAIL and the death receptors DR4 and DR5 correlates with progression of degeneration in human intervertebral disks. | |||||||||||||||
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