INT119203

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Context Info
Confidence 0.74
First Reported 2003
Last Reported 2010
Negated 3
Speculated 2
Reported most in Body
Documents 64
Total Number 80
Disease Relevance 11.37
Pain Relevance 4.41

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

extracellular space (CXADR) extracellular region (CXADR) cell adhesion (CXADR)
mitochondrion organization (CXADR) plasma membrane (CXADR) nucleus (CXADR)
Anatomy Link Frequency
CAR 46
liver 4
kidney 3
neurons 3
brain 2
CXADR (Homo sapiens)
Pain Link Frequency Relevance Heat
Substantia nigra 270 98.92 Very High Very High Very High
Dopamine 270 98.88 Very High Very High Very High
imagery 148 97.48 Very High Very High Very High
Paracetamol 41 94.64 High High
isoflurane 27 92.16 High High
midbrain 45 91.52 High High
anesthesia 27 91.28 High High
opioid receptor 8 90.72 High High
agonist 90 87.76 High High
Central nervous system 140 85.52 High High
Disease Link Frequency Relevance Heat
Targeted Disruption 352 99.98 Very High Very High Very High
Cancer 612 99.96 Very High Very High Very High
Malignant Neoplastic Disease 14 99.58 Very High Very High Very High
Infection 450 98.72 Very High Very High Very High
Head & Neck Cancer 16 95.80 Very High Very High Very High
Hepatotoxicity 1 92.88 High High
Parkinson's Disease 225 86.88 High High
Disease 90 84.56 Quite High
Fever 20 82.80 Quite High
Viral Infection 24 81.68 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Correct splicing of exon 9, which is a prerequisite of functional CAR expression, is restricted to only a few tissues, among them small intestine and liver where CAR function has been demonstrated [5,25].
Gene_expression (expression) of CAR in small intestine
1) Confidence 0.74 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
Fig. 3B shows that all CAR protein isoforms were expressed at comparable levels in vitro.
Gene_expression (expressed) of CAR in CAR
2) Confidence 0.74 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0.04
CAR is predominantly expressed in liver and in the intestinal epithelium [4,5].
Gene_expression (expressed) of CAR in intestinal epithelium
3) Confidence 0.74 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0.08
O.B. conceived of and directed the study, carried out the studies of CAR expression in human cell lines and prepared the manuscript.
Gene_expression (expression) of CAR in CAR
4) Confidence 0.74 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
K.A.A. carried out the experiments to functionally characterize CAR variant proteins and to analyze expression of CAR transcripts in human tissues and drafted the manuscript.
Spec (analyze) Gene_expression (expression) of CAR in CAR
5) Confidence 0.74 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
Consequently, we were interested in the expression pattern of CAR splicing variants in different tissues.
Gene_expression (expression) of CAR in CAR
6) Confidence 0.74 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
The constitutive expression of these transcripts will be very low in general, as in many tissues and cell lines Northern blot analysis did not detect any CAR expression or only a very weak one (ref. [4], and Fig. 8A).
Gene_expression (expression) of CAR in CAR
7) Confidence 0.74 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
Co-transfection of reference CAR(SV1) expression plasmid resulted in 11-fold activation of CYP2B6 reporter, 7-fold activation of CYP3A4 reporter and 12-fold activation of MDR1 reporter, thus demonstrating constitutive transcriptional activity of reference CAR(SV1) in COS1 cells (Fig. 4).
Gene_expression (expression) of CAR in CAR
8) Confidence 0.65 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0.03
Expression of CAR transcripts was analyzed by PCR with the primers listed in Table 1.
Spec (analyzed) Gene_expression (Expression) of CAR in CAR
9) Confidence 0.64 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0.04
The eukaryotic expression plasmid for CAR(SV5) was constructed by cloning the 910 bp EcoRI/XmaI insert fragment of pcDhCAR(SV1) and the 130 bp XmaI/HindIII insert fragment of a pGEM exon 8–9 clone (see above) containing the deletion in exon 9 into appropriately digested vector pcDNA3.1(-) (Invitrogen).
Gene_expression (expression) of CAR in CAR
10) Confidence 0.64 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
For Western blot analysis of CAR isoforms, COS1 cells were co-transfected with the respective expression plasmids and ?
Gene_expression (transfected) of CAR in CAR
11) Confidence 0.64 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
Human CAR isoforms and RXR?
Gene_expression (isoforms) of CAR in CAR
12) Confidence 0.64 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
protein were synthesized using the CAR isoform expression plasmids and pCMX-hRXR?
Gene_expression (expression) of CAR in CAR
13) Confidence 0.64 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
RT-PCR with primers F3/R3 (Table 1), targeting exons 2/3 which encode parts of the DBD, confirmed the ubiquitous expression of CAR transcripts in human tissues (data not shown).
Gene_expression (expression) of CAR in CAR
14) Confidence 0.64 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
The major CAR transcripts expressed in human and mouse liver are in the size range of 1.3–1.7 kb [4].
Gene_expression (expressed) of CAR in liver
15) Confidence 0.64 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0.04
We propose that the tissue- and differentiation state-specific correct splicing of exon 8 to exon 9 may be a transcription-coupled mechanism to restrict expression of functional CAR to the relevant target sites.
Gene_expression (expression) of CAR in CAR
16) Confidence 0.58 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
Since we could demonstrate tissue-specific alternative splicing of CAR, it was of interest to see whether alternative splicing may also be associated with the induction of CAR expression by differentiation.
Gene_expression (expression) of CAR in CAR
17) Confidence 0.58 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
As we demonstrated, that most CAR transcripts encode non-functional isoforms, we investigated which tissues may express transcripts encoding functional CAR proteins.
Gene_expression (express) of CAR in CAR
18) Confidence 0.58 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
Thus, transcripts encoding functional CAR protein are not expressed in most tissues.
Neg (not) Gene_expression (expressed) of CAR in CAR
19) Confidence 0.58 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0
Fig. 7A shows that CAR expression cannot be detected by Northern blot in intestinal cell lines including subconfluent and confluent Caco-2 TC7.
Gene_expression (expression) of CAR in Caco-2
20) Confidence 0.58 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0

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