INT119219

Context Info Confidence 0.64 First Reported 2004 Last Reported 2009 Negated 0 Speculated 0 Reported most in Body Documents 3 Total Number 3 Disease Relevance 0.16 Pain Relevance 1.50

plasma membrane (Dlg4) protein complex assembly (Dlg4) response to stress (Dlg4) cytoplasm (Dlg4)

Anatomy Link Frequency shell 1 shafts 1 Dlg4 (Rattus norvegicus)

Pain Link Frequency Relevance Heat gABA 5 99.26 Morphine 9 98.00 Nucleus accumbens 7 97.28 Hippocampus 25 90.64 dopamine receptor 2 89.16 long-term potentiation 9 83.72 Action potential 5 79.76 Dopamine 1 40.52 opiate 1 36.92 imagery 20 29.84

Disease Link Frequency Relevance Heat Apoptosis 1 85.40 Shock 4 72.32 Amnesia 3 5.00 Anxiety Disorder 2 5.00 Pathologic Processes 2 5.00 Decapitation 2 5.00 Stress 2 5.00 Toxicity 1 5.00 Nervous System Malformation 1 5.00 Aging 1 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

In practically all experiments, we observed a gradual increase in the total number of PSD-95:GFP puncta, mainly (but not exclusively) due to increased density of PSD-95:GFP puncta along existing dendritic segments, at both spine tips and shafts (Figures 1C and 3B).

Localization (number) of PSD-95 in shafts

1) Confidence 0.64 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2693930 Disease Relevance 0 Pain Relevance 0.10

Specifically, GABA-A alpha1 subunit, GRIA1 subunit and PSD-95 mRNAs were decreased in the shell but increased in the core following morphine administration.

Localization (decreased) of PSD-95 in shell associated with gaba and morphine

2) Confidence 0.35 Published 2004 Journal Neuroscience Section Abstract Doc Link 15183518 Disease Relevance 0.09 Pain Relevance 1.22

Our findings are consistent with others showing that, in vitro, BDNF upregulates local translation of PSD95 through mTOR pathway [76] and enhances the expression of GluR1 subunit of AMPA receptors via activation of mTOR [48].

Localization (translation) of PSD95

3) Confidence 0.31 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2695538 Disease Relevance 0.07 Pain Relevance 0.18

General Comments

This test has worked.