INT120509
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Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
In contrast, bronchiolar epithelial cells in fibrotic lungs showed decreased catalase expression to various degrees (Figure 2B). | |||||||||||||||
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In the lungs, catalase is expressed during the later stages of development, is constitutively expressed in airway and alveolar epithelial cells and in macrophages [10-12], and plays an important role in the endogenous antioxidant defense system. | |||||||||||||||
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For example, treatment with a catalase/superoxide dismutase mimetic, or adenoviral-mediated overexpression of catalase, inhibits hydrogen peroxide-stimulated EMMPRIN upregulation in cardiac myocytes [38]. | |||||||||||||||
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The significance of catalase mRNA expression among the groups persisted at 7 and 14 days when normalized by BGUS (p < 0.05, respectively). | |||||||||||||||
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The significance of catalase mRNA expression between the two groups persisted when normalized by 18s rRNA (0.62 ± 0.1 vs. 1.6 ± 0.1, p = 0.0002). | |||||||||||||||
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Further to this idea, in one study a transgenic mouse overexpressing catalase localized to mitochondria showed an extended life span due to enhanced protection of mitochondria from reactive oxygen species (ROS), in which catalase overexpression also suppressed age-related DNA oxidation in skeletal muscle [7]. | |||||||||||||||
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Catalase mRNA was present in bronchiolar epithelial cells, and the expression levels were significantly lower at 7 days after bleomycin administration compared with untreated lungs (p = 0.009) (Figure 3C).
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In the lungs, catalase is expressed during the later stages of development, is constitutively expressed in airway and alveolar epithelial cells and in macrophages [10-12], and plays an important role in the endogenous antioxidant defense system. | |||||||||||||||
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To assess whether the reduction in catalase activity in fibrotic lungs is due to the decreased synthesis of catalase, we quantified catalase expression in lung tissues using Western blotting and quantitative RT-PCR. | |||||||||||||||
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Using LCM we harvested bronchiolar epithelial cells from lungs in order to quantify catalase mRNA expression in vivo, as previously described [18]. | |||||||||||||||
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We then examined the dynamic change in bronchiolar catalase expression following administration of bleomycin in mice. | |||||||||||||||
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To assess whether the reduction in catalase activity in fibrotic lungs is due to the decreased synthesis of catalase, we quantified catalase expression in lung tissues using Western blotting and quantitative RT-PCR. | |||||||||||||||
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We observed that catalase is predominantly expressed in bronchiolar epithelium in normal lungs, and is diminished in IP lungs, especially in bronchiolar epithelium and in abnormal re-epithelialization, such as bronchiolization and squamous metaplasia in humans (Figure 2). | |||||||||||||||
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To evaluate the effects of Lb. casei BL23 producing or not MnKat catalase on inflammation development, two groups of mice received 5 × 109 cfu/mouse of either Lb. casei MnKat- or Lb. casei MnKat+ by intragastric administration one day before starting DSS administration and then daily until sacrifice. | |||||||||||||||
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Glutathione-S-transferase-Pi and catalase were overexpressed in the lungs of mice exposed to light only. | |||||||||||||||
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Firstly, we investigated the contribution of brain catalase in the acquisition of ethanol-induced conditioned place preference (CPP). | |||||||||||||||
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METHODS: Swiss male mice received an intraperitoneal injection of the catalase inhibitor 3-amino-1,2,4-triazole (AT; 0-1 g/kg), and 0 to 20 hr after this administration, animals received an ethanol (0-4 g/kg; intraperitoneally) challenge. | |||||||||||||||
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It also should be noted that fibroblastic foci were exclusively negative for catalase in fibrotic lungs. | |||||||||||||||
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Contamination by red blood cells does not contribute to catalase activity of the lungs | |||||||||||||||
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For example, treatment with a catalase/superoxide dismutase mimetic, or adenoviral-mediated overexpression of catalase, inhibits hydrogen peroxide-stimulated EMMPRIN upregulation in cardiac myocytes [38]. | |||||||||||||||
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