INT120509

Context Info Confidence 0.77 First Reported 2003 Last Reported 2011 Negated 3 Speculated 2 Reported most in Body Documents 65 Total Number 89 Disease Relevance 50.90 Pain Relevance 9.75

cytosol (Cat) mitochondrion (Cat) oxidoreductase activity (Cat) Golgi apparatus (Cat) endoplasmic reticulum (Cat) plasma membrane (Cat)

Anatomy Link Frequency lungs 11 liver 4 cardiac myocytes 3 macrophages 2 skeletal muscle 2 Cat (Mus musculus)

Pain Link Frequency Relevance Heat Inflammation 1256 99.84 fibrosis 1298 99.48 Arthritis 124 98.40 agonist 71 97.12 acular 27 96.68 Demyelination 130 96.26 ischemia 63 94.24 Paracetamol 14 93.36 tolerance 72 90.32 Potency 14 90.20

Disease Link Frequency Relevance Heat Targeted Disruption 237 99.98 Mycobacterial Infection 145 99.98 Death 136 99.92 Sprains And Strains 276 99.88 Infection 140 99.88 INFLAMMATION 1196 99.84 Fibrosis 1020 99.84 Stress 783 99.66 Pulmonary Fibrosis 668 99.48 Diabetes Mellitus 284 99.36

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Mutation

Event

Anatomy

Negation

Speculation

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In contrast, bronchiolar epithelial cells in fibrotic lungs showed decreased catalase expression to various degrees (Figure 2B).

Gene_expression (expression) of catalase in epithelial cells associated with fibrosis

1) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.56 Pain Relevance 0

In the lungs, catalase is expressed during the later stages of development, is constitutively expressed in airway and alveolar epithelial cells and in macrophages [10-12], and plays an important role in the endogenous antioxidant defense system.

Gene_expression (expressed) of catalase in macrophages

2) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.92 Pain Relevance 0.15

For example, treatment with a catalase/superoxide dismutase mimetic, or adenoviral-mediated overexpression of catalase, inhibits hydrogen peroxide-stimulated EMMPRIN upregulation in cardiac myocytes [38].

Gene_expression (overexpression) of catalase in cardiac myocytes

3) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.59 Pain Relevance 0.26

The significance of catalase mRNA expression among the groups persisted at 7 and 14 days when normalized by BGUS (p < 0.05, respectively).

Gene_expression (expression) of catalase mRNA

4) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.36 Pain Relevance 0.15

The significance of catalase mRNA expression between the two groups persisted when normalized by 18s rRNA (0.62 ± 0.1 vs. 1.6 ± 0.1, p = 0.0002).

Gene_expression (expression) of catalase mRNA

5) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.70 Pain Relevance 0.07

Further to this idea, in one study a transgenic mouse overexpressing catalase localized to mitochondria showed an extended life span due to enhanced protection of mitochondria from reactive oxygen species (ROS), in which catalase overexpression also suppressed age-related DNA oxidation in skeletal muscle [7].

Gene_expression (overexpression) of catalase in skeletal muscle associated with targeted disruption

6) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.71 Pain Relevance 0.15

Catalase mRNA was present in bronchiolar epithelial cells, and the expression levels were significantly lower at 7 days after bleomycin administration compared with untreated lungs (p = 0.009) (Figure 3C).

Gene_expression (present) of Catalase mRNA in lungs

7) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.23 Pain Relevance 0.07

In the lungs, catalase is expressed during the later stages of development, is constitutively expressed in airway and alveolar epithelial cells and in macrophages [10-12], and plays an important role in the endogenous antioxidant defense system.

Gene_expression (expressed) of catalase in macrophages

8) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.84 Pain Relevance 0.12

To assess whether the reduction in catalase activity in fibrotic lungs is due to the decreased synthesis of catalase, we quantified catalase expression in lung tissues using Western blotting and quantitative RT-PCR.

Gene_expression (expression) of catalase in lung associated with fibrosis

9) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.58 Pain Relevance 0.08

Using LCM we harvested bronchiolar epithelial cells from lungs in order to quantify catalase mRNA expression in vivo, as previously described [18].

Gene_expression (expression) of catalase mRNA in lungs

10) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.23 Pain Relevance 0.07

We then examined the dynamic change in bronchiolar catalase expression following administration of bleomycin in mice.

Gene_expression (expression) of catalase

11) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.23 Pain Relevance 0.07

To assess whether the reduction in catalase activity in fibrotic lungs is due to the decreased synthesis of catalase, we quantified catalase expression in lung tissues using Western blotting and quantitative RT-PCR.

Gene_expression (synthesis) of catalase in lung associated with fibrosis

12) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.58 Pain Relevance 0.09

We observed that catalase is predominantly expressed in bronchiolar epithelium in normal lungs, and is diminished in IP lungs, especially in bronchiolar epithelium and in abnormal re-epithelialization, such as bronchiolization and squamous metaplasia in humans (Figure 2).

Gene_expression (expressed) of catalase in lungs associated with metaplasia

13) Confidence 0.77 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.37 Pain Relevance 0.14

To evaluate the effects of Lb. casei BL23 producing or not MnKat catalase on inflammation development, two groups of mice received 5 × 109 cfu/mouse of either Lb. casei MnKat- or Lb. casei MnKat+ by intragastric administration one day before starting DSS administration and then daily until sacrifice.

Gene_expression (producing) of MnKat catalase associated with inflammation

14) Confidence 0.70 Published 2007 Journal Microb Cell Fact Section Body Doc Link PMC1949835 Disease Relevance 0.75 Pain Relevance 0.15

Glutathione-S-transferase-Pi and catalase were overexpressed in the lungs of mice exposed to light only.

Gene_expression (overexpressed) of catalase in lungs

15) Confidence 0.69 Published 2004 Journal FASEB J. Section Abstract Doc Link 15289447 Disease Relevance 0.30 Pain Relevance 0.05

Firstly, we investigated the contribution of brain catalase in the acquisition of ethanol-induced conditioned place preference (CPP).

Spec (investigated) Gene_expression (contribution) of catalase in brain

16) Confidence 0.67 Published 2008 Journal Physiol. Behav. Section Abstract Doc Link 18155096 Disease Relevance 0.08 Pain Relevance 0.21

METHODS: Swiss male mice received an intraperitoneal injection of the catalase inhibitor 3-amino-1,2,4-triazole (AT; 0-1 g/kg), and 0 to 20 hr after this administration, animals received an ethanol (0-4 g/kg; intraperitoneally) challenge.

Gene_expression (injection) of catalase

17) Confidence 0.67 Published 2004 Journal Alcohol. Clin. Exp. Res. Section Body Doc Link 15608607 Disease Relevance 0 Pain Relevance 0

It also should be noted that fibroblastic foci were exclusively negative for catalase in fibrotic lungs.

Neg (negative) Gene_expression (negative) of catalase in lungs associated with fibrosis

18) Confidence 0.67 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.67 Pain Relevance 0.24

Contamination by red blood cells does not contribute to catalase activity of the lungs

Gene_expression (activity) of catalase in lungs

19) Confidence 0.67 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.49 Pain Relevance 0.03

For example, treatment with a catalase/superoxide dismutase mimetic, or adenoviral-mediated overexpression of catalase, inhibits hydrogen peroxide-stimulated EMMPRIN upregulation in cardiac myocytes [38].

Gene_expression (overexpression) of catalase in cardiac myocytes

20) Confidence 0.67 Published 2010 Journal Respir Res Section Body Doc Link PMC3022724 Disease Relevance 0.60 Pain Relevance 0.27

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