INT12325

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Context Info
Confidence 0.72
First Reported 1992
Last Reported 2009
Negated 4
Speculated 1
Reported most in Body
Documents 47
Total Number 48
Disease Relevance 11.92
Pain Relevance 13.35

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytoskeletal protein binding (Plec) plasma membrane (Plec) cytoskeleton (Plec)
cytoplasm (Plec)
Anatomy Link Frequency
nucleus 4
fibroblasts 2
poly 2
liver 1
neurons 1
Plec (Mus musculus)
Pain Link Frequency Relevance Heat
Dynorphin 2200 99.92 Very High Very High Very High
Opioid 520 99.82 Very High Very High Very High
Paracetamol 16 99.80 Very High Very High Very High
agonist 329 99.72 Very High Very High Very High
MU agonist 60 99.44 Very High Very High Very High
opioid receptor 200 98.82 Very High Very High Very High
Endogenous opioid 100 98.00 Very High Very High Very High
Morphine 120 97.52 Very High Very High Very High
dexamethasone 27 97.04 Very High Very High Very High
imagery 1095 92.48 High High
Disease Link Frequency Relevance Heat
Adenocarcinoma 1584 99.96 Very High Very High Very High
Bacterial Infection 23 99.04 Very High Very High Very High
Pancreatic Cancer 336 98.92 Very High Very High Very High
Cancer 861 98.68 Very High Very High Very High
Stress 467 97.44 Very High Very High Very High
Viral Infection 8 97.44 Very High Very High Very High
Flushing 120 96.72 Very High Very High Very High
Hepatotoxicity 11 95.96 Very High Very High Very High
Injury 104 94.04 High High
Disease 159 93.96 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The discovery that plectin-1 (a cytoskeletal component) is abnormally expressed on the cell surface of PDACs provides new information about the development of pancreatic cancer that could eventually lead to new ways to treat this disease.
Gene_expression (expressed) of plectin-1 associated with pancreatic cancer and disease
1) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Abstract Doc Link PMC2292750 Disease Relevance 1.01 Pain Relevance 0.17
HUVECs had very low levels of plectin-1 expression in the nucleus.
Gene_expression (expression) of plectin-1 in nucleus
2) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.41 Pain Relevance 0
Normal animals had scattered plectin-1 staining, whereas in PanINs and PDAC, plectin-1 was expressed in the lesions but not in the surrounding tissue (Figure 4E).
Neg (not) Gene_expression (expressed) of plectin-1 associated with adenocarcinoma
3) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.50 Pain Relevance 0.07
Normal mouse pancreatic ductal cells had low levels of plectin-1 expression, whereas normal human pancreatic ductal cells had plectin-1 expression in the cytoplasm and nucleus but not on the membrane (Figure 4D).
Gene_expression (expression) of plectin-1 in nucleus
4) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.53 Pain Relevance 0
Plectin-1 is present in the membrane as well as the cytoplasm of both murine and human PDAC cells (Figure 4D).
Gene_expression (present) of Plectin-1 associated with adenocarcinoma
5) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.56 Pain Relevance 0
As a control for plectin-1 expression, we used NIH-3T3 cells, which are known to have plectin-1 in the cytoplasm and nucleus but not on the cell surface [29].
Gene_expression (expression) of plectin-1 in nucleus
6) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.46 Pain Relevance 0
Normal mouse pancreatic ductal cells had low levels of plectin-1 expression, whereas normal human pancreatic ductal cells had plectin-1 expression in the cytoplasm and nucleus but not on the membrane (Figure 4D).
Gene_expression (expression) of plectin-1 in nucleus
7) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.46 Pain Relevance 0
To produce plectin-1 targeted or control nanoparticles, succinimidyl iodoacetic acid was reacted with CLIO-Cy5.5 for 15 min, purified by size exclusion chromatography, then reacted with peptidyl-cysteine for 1 h.
Gene_expression (produce) of plectin-1
8) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0 Pain Relevance 0.03
Plectin-1 targeted peptide (PTP) (amino acid sequence: KTLLPTP) or control peptide were synthesized by Tufts Peptide Core Facility with a GGSK(FITC)C linker for conjugation of the peptide to a model fluorescent nanoparticle (crosslinked iron oxides [CLIO]-Cy5.5).
Gene_expression (synthesized) of Plectin-1 in Tufts
9) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.09 Pain Relevance 0
As was expected, plectin-1 was absent from the membrane but present in the cytoplasmic and nuclear fractions of fibroblasts (Figure 4D).
Neg (absent) Gene_expression (absent) of plectin-1 in fibroblasts
10) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.42 Pain Relevance 0
All fractions were analyzed for plectin-1 expression via Western blotting.


Spec (analyzed) Gene_expression (expression) of plectin-1
11) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.15 Pain Relevance 0
Biodistribution studies revealed specific uptake in tumors with minimal uptake in muscle or skin, two tissues with reported plectin-1 expression (Figure 5D).
Gene_expression (expression) of plectin-1 in skin associated with cancer
12) Confidence 0.72 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.64 Pain Relevance 0.21
Serial frozen sections were stained with HE or for the presence of M13 (Amersham Biosciences) or plectin-1.
Gene_expression (presence) of plectin-1
13) Confidence 0.62 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.27 Pain Relevance 0.07
We have shown that plectin-1 levels are low in normal pancreatic ductal cells, but its expression is upregulated in PanINs and remains elevated in PDAC.
Gene_expression (levels) of plectin-1 associated with adenocarcinoma
14) Confidence 0.62 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.46 Pain Relevance 0.09
Finally, the researchers attached this plectin-1–targeted peptide (PTP) to a nanoparticles that was both magnetic and fluorescent (PTP-NP) and used special microscopy (which detects the fluorescent part of this very small particle) and MRI (which detects its magnetic portion) to show that this potential imaging probe was found in areas of PDAC (but not in normal pancreatic tissue) in the mouse model of human PDAC.


Gene_expression (attached) of plectin-1 associated with adenocarcinoma and imagery
15) Confidence 0.62 Published 2008 Journal PLoS Medicine Section Abstract Doc Link PMC2292750 Disease Relevance 0.99 Pain Relevance 0.16
As was expected, plectin-1 was absent from the membrane but present in the cytoplasmic and nuclear fractions of fibroblasts (Figure 4D).
Neg (absent) Gene_expression (present) of plectin-1 in fibroblasts
16) Confidence 0.56 Published 2008 Journal PLoS Medicine Section Body Doc Link PMC2292750 Disease Relevance 0.48 Pain Relevance 0
Since the PCN was expressed and purified from an E. coli heterologous expression system, it is most likely to contain residual contaminating LPS.
Gene_expression (expressed) of PCN
17) Confidence 0.36 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2743193 Disease Relevance 0.92 Pain Relevance 0
DEX decreased the glutathione concentration (36%) in liver and increased the biliary excretion of AA-GSH, which reflects the activation of AA, whereas PCN produced neither effect.
Neg (neither) Gene_expression (produced) of PCN in liver associated with paracetamol and dexamethasone
18) Confidence 0.35 Published 1992 Journal Toxicol. Appl. Pharmacol. Section Abstract Doc Link 1641853 Disease Relevance 0.82 Pain Relevance 1.64
It is worth noting in the context of analogies between the podocyte slit diaphragm and neuronal dendrites that numerous mRNAs that encode dendritic proteins are themselves localized to dendritic spines where they appear to be dynamically translated at their sites of expression [60] Just as a number of the proteins encoded by these mRNA are localized to dendrites, it is also noteworthy that 15 of the enriched mRNAs themselves have been shown to localize to dendritic spines, the post-synaptic density, or (in one case) at the post-synaptic membrane of the neuromuscular junction: Ppp1r9b, Dbn1, Myh9, Plec1, Spna2, Spnb2, Actn4, Actn1, Gsn, Nes, Pls3, Utrn, Dst, Myo1b, Shrm [61]–[69].
Gene_expression (expression) of Plec1 in spines
19) Confidence 0.19 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2714980 Disease Relevance 0 Pain Relevance 0
On day 12, EBs expressing DsRed-Nuc under the ?
Gene_expression (expressing) of EBs
20) Confidence 0.09 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2483414 Disease Relevance 0 Pain Relevance 0

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