INT123530

Context Info Confidence 0.80 First Reported 2004 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 9 Total Number 10 Disease Relevance 3.46 Pain Relevance 1.99

cytosol (RELA) nucleoplasm (RELA) aging (RELA) nucleus (RELA) DNA binding (RELA) transcription factor binding (RELA)

Anatomy Link Frequency SH-SY5Y 2 RELA (Homo sapiens)

Pain Link Frequency Relevance Heat fluoxetine 7 99.34 opioid receptor 8 99.02 Inflammation 97 98.68 Kinase C 2 95.96 cytokine 34 93.40 Inflammatory stimuli 4 90.56 Opioid 2 86.48 Osteoarthritis 38 84.28 ischemia 2 81.76 antidepressant 1 73.00

Disease Link Frequency Relevance Heat Death 21 99.72 Neuroblastoma 6 99.60 INFLAMMATION 120 98.68 Apoptosis 15 93.04 Ovarian Cancer 3 90.08 Bordatella Infection 2 90.08 Osteoarthritis 38 84.28 Reperfusion Injury 1 82.52 Cv Unclassified Under Development 1 81.76 Renal Disease 1 79.60

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

We performed Western blot analysis of the total protein lysate from the tissues of mouse PIN lesions to measure the level of expression of androgen receptor, vascular endothelial growth factor, nuclear factor-kappaB p65, BclII, AKT (total and phosphorylated Ser473), p53, cyclin-dependent kinase inhibitor p21WAF1/CIP1, p27, BAX, and caspase-3 to demonstrate the COX-2-independent mechanism involved in the inhibition of PIN lesions of the dorsolateral prostate by both celecoxib and exisulind.

Phosphorylation (phosphorylated) of p65

1) Confidence 0.80 Published 2004 Journal Clin. Cancer Res. Section Body Doc Link 15570007 Disease Relevance 0.07 Pain Relevance 0

Oxidant scavengers and Bay 11-7085 [an inhibitor of nuclear factor kappaB (NF-kappaB) activation] prevented the FLX-induced cell death, increase in phosphorylated inhibitory kappaB-alpha and NF-kappaB p65 levels, and binding of NF-kappaB p65 to DNA.

Phosphorylation (phosphorylated) of p65 associated with death and fluoxetine

2) Confidence 0.61 Published 2010 Journal Basic Clin. Pharmacol. Toxicol. Section Abstract Doc Link 20050848 Disease Relevance 0.74 Pain Relevance 0.61

phosphorylation and on p65 nuclear translocation.

Phosphorylation (phosphorylation) of p65

3) Confidence 0.61 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875647 Disease Relevance 0.22 Pain Relevance 0.13

phosphorylation and p65 nuclear migration

Phosphorylation (phosphorylation) of p65

4) Confidence 0.46 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875647 Disease Relevance 0 Pain Relevance 0

Here we demonstrate that activation of the mu-opioid receptor in human neuroblastoma SH-SY5Y cells led to the phosphorylations of IkappaB kinase (IKK) and p65, denoting the stimulation of the nuclear factor-kappaB (NFkappaB) transcription factor.

Phosphorylation (phosphorylations) of p65 in SH-SY5Y associated with neuroblastoma and opioid receptor

5) Confidence 0.40 Published 2005 Journal Neurosignals Section Abstract Doc Link 16088228 Disease Relevance 0.26 Pain Relevance 0.28

These data suggest that the mu-opioid receptor is capable of simulating NFkappaB signaling via the phosphorylation of IKK and p65 in human neuroblastoma SH-SY5Y cells.

Phosphorylation (phosphorylation) of p65 in SH-SY5Y associated with neuroblastoma and opioid receptor

6) Confidence 0.40 Published 2005 Journal Neurosignals Section Abstract Doc Link 16088228 Disease Relevance 0.33 Pain Relevance 0.29

B transcriptional activity through a p38 MAPK-dependent RelA phosphorylation pathway in the induction of pro-inflammatory gene expression [40].

Phosphorylation (phosphorylation) of RelA associated with inflammation

7) Confidence 0.27 Published 2008 Journal J Inflamm (Lond) Section Body Doc Link PMC2442592 Disease Relevance 0.48 Pain Relevance 0.13

Exposure of Granta cells to 30 µM parthenolide for 3 hours resulted in a significant decrease in the phosphorylation state of p65 (Fig. 6B, lane 2) which was abrogated by pretreatment with 5 mM GSH for 2 hours (Fig. 6B, lane 4).

Phosphorylation (phosphorylation) of p65

8) Confidence 0.13 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2780735 Disease Relevance 0.12 Pain Relevance 0

The now active PKAC subunit dissociates and phosphorylates the p65 subunit of NF-?

Phosphorylation (phosphorylates) of p65 subunit

9) Confidence 0.09 Published 2007 Journal Immun Ageing Section Body Doc Link PMC1845171 Disease Relevance 0.25 Pain Relevance 0.20

Resveratrol, like N-Ac-Leu-Leu-norleucinal (ALLN) suppressed IL-1beta-induced proteasome function and the degradation of IkappaBalpha (an inhibitor of NF-kappaB) without affecting IkappaBalpha kinase activation, IkappaBalpha-phosphorylation or IkappaBalpha-ubiquitination which suppressed nuclear translocation of the p65 subunit of NF-kappaB and its phosphorylation.

Phosphorylation (phosphorylation) of p65 subunit

10) Confidence 0.05 Published 2008 Journal Biochem. Pharmacol. Section Abstract Doc Link 18606398 Disease Relevance 0.98 Pain Relevance 0.34

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