INT129960

Context Info Confidence 0.53 First Reported 2005 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 19 Total Number 20 Disease Relevance 7.17 Pain Relevance 3.55

peptidase activity (MMP13) extracellular space (MMP13) extracellular region (MMP13) Golgi apparatus (MMP13) proteinaceous extracellular matrix (MMP13) lysosome (MMP13)

Anatomy Link Frequency chondrocytes 8 granulocyte 2 articular 2 blood 2 cartilage 2 MMP13 (Homo sapiens)

Pain Link Frequency Relevance Heat metalloproteinase 120 100.00 Angina 12 99.88 Osteoarthritis 647 99.44 licofelone 6 97.26 rheumatoid arthritis 135 93.84 cytokine 100 91.44 Inflammation 151 90.08 Inflammatory response 4 74.92 COX2 2 72.72 Pain 68 59.12

Disease Link Frequency Relevance Heat Hypertrophy 24 100.00 Hypercalcemia 37 99.92 Cv General 3 Under Development 12 99.88 Osteoarthritis 678 99.44 Repression 15 99.16 Coronary Artery Disease 7 98.32 Arthritis 108 94.24 Rheumatoid Arthritis 138 93.84 Atherosclerotic Plaque 1 90.20 INFLAMMATION 168 90.08

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Ciprofloxacin, norfloxacin and ofloxacin each reduced both basal and stimulated expression of MMP-13 mRNA.

Negative_regulation (reduced) of Gene_expression (expression) of MMP-13 mRNA

1) Confidence 0.53 Published 2005 Journal Rheumatology (Oxford) Section Body Doc Link 16148020 Disease Relevance 0 Pain Relevance 0

Given that HDAC activity is typically associated with transcriptional repression [47], this result is consistent with the decrease in MMP-1 and MMP-13 gene expression in cells treated with a combination of both ligands, suggesting that the compounds may be inhibiting expression of these genes through a common histone acetylation-associated mechanism.

Negative_regulation (decrease) of Gene_expression (expression) of MMP-13 associated with repression

2) Confidence 0.40 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2656243 Disease Relevance 0.16 Pain Relevance 0.09

Figure 3 shows a decrease in expression of MMP-1 and MMP-13 at the transcriptional level in cells treated with LG268 and rosiglitazone.

Negative_regulation (decrease) of Gene_expression (expression) of MMP-13

3) Confidence 0.40 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2656243 Disease Relevance 0 Pain Relevance 0

ligand rosiglitazone suppresses MMP-1 and MMP13 gene expression more effectively than either compound alone.

Negative_regulation (suppresses) of Gene_expression (expression) of MMP13

4) Confidence 0.40 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2656243 Disease Relevance 0.13 Pain Relevance 0.03

As a result, combined treatment should lead to greater inhibition of MMP-1 and MMP-13 gene expression compared with either compound alone.

Negative_regulation (inhibition) of Gene_expression (expression) of MMP-13

5) Confidence 0.40 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2656243 Disease Relevance 0.13 Pain Relevance 0.03

, ligands block MMP-1 and MMP-13 gene expression, RXR:PPAR?

Negative_regulation (block) of Gene_expression (expression) of MMP-13

6) Confidence 0.40 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2656243 Disease Relevance 0.15 Pain Relevance 0.04

, and MMP13 gene expression decreased.

Negative_regulation (decreased) of Gene_expression (expression) of MMP13

7) Confidence 0.37 Published 2010 Journal BMC Complement Altern Med Section Abstract Doc Link PMC2921357 Disease Relevance 0.06 Pain Relevance 0

Pretreatment with 2.5 and 10 mmol/l glucosamine was able, to differing degrees, to downregulate mRNA and protein levels of MMP-1, MMP-3 and MMP-13.

Negative_regulation (downregulate) of Gene_expression (levels) of MMP-13

8) Confidence 0.35 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC2212570 Disease Relevance 0.32 Pain Relevance 0.12

Interestingly, the most potent inhibitor of MMP-1 and MMP-13 production was LY83583, an inhibitor of NO-dependent soluble guanylate cyclase and of cGMP.

Negative_regulation (inhibitor) of Gene_expression (production) of MMP-13

9) Confidence 0.33 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1065327 Disease Relevance 0.17 Pain Relevance 0

In the present study, subtle differences are shown in the pattern of inhibition of the ET-1-induced overproduction of MMP-1 and MMP-13.

Negative_regulation (inhibition) of Gene_expression (overproduction) of MMP-13

10) Confidence 0.33 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1065327 Disease Relevance 0.47 Pain Relevance 0.16

Treatment group MMP-13 gene expression decreased to 0.55 (p = 0.19), and TNF?

Negative_regulation (decreased) of Gene_expression (expression) of MMP-13

11) Confidence 0.32 Published 2010 Journal BMC Complement Altern Med Section Body Doc Link PMC2921357 Disease Relevance 0.22 Pain Relevance 0.06

To elucidate whether MMP-1, MMP-8 and MMP-13 levels in blood were high in patients with unstable angina (UAP), we measured serum MMP-1 and plasma MMP-8 and MMP-13 levels in 45 patients with UAP, 175 with stable coronary artery disease (CAD), and 45 controls.

Negative_regulation (measured) of Gene_expression (levels) of MMP-13 in blood associated with angina and coronary artery disease

12) Confidence 0.31 Published 2010 Journal Atherosclerosis Section Abstract Doc Link 19674746 Disease Relevance 1.15 Pain Relevance 0.84

The group of patients receiving 1 mg of sCT exhibited significant decreases in the levels of CTX-II, C2C, and MMP-13.

Negative_regulation (decreases) of Gene_expression (levels) of MMP-13

13) Confidence 0.18 Published 2006 Journal Arthritis Rheum. Section Body Doc Link 17009253 Disease Relevance 0.06 Pain Relevance 0

In comparison with controls, OA explants from five patients cultured in the presence of 10 pg/ml PGE2 showed decreased expression of the genes related to chondrocyte hypertrophy, namely those encoding COL10A1 and MMP-13 (Figure 3).

Negative_regulation (decreased) of Gene_expression (expression) of MMP-13 in chondrocyte associated with hypertrophy, metalloproteinase and osteoarthritis

14) Confidence 0.17 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC2206385 Disease Relevance 0.51 Pain Relevance 0.41

In the same line of thought, a study performed by Legendre and colleagues [32] recently demonstrated a similar inhibitory effect of rhein on MMP-13 production in articular chondrocytes.

Negative_regulation (effect) of Gene_expression (production) of MMP-13 in articular

15) Confidence 0.16 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2483463 Disease Relevance 0.47 Pain Relevance 0.28

Our results showed that EGCG pretreatment of cultured human OA chondrocytes significantly inhibited the expression and activities of MMP-1 and MMP-13 in a dose-dependent manner [21].

Negative_regulation (inhibited) of Gene_expression (expression) of MMP-13 in chondrocytes associated with osteoarthritis

16) Confidence 0.10 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2888220 Disease Relevance 0.43 Pain Relevance 0.27

These findings are in accordance with a study conducted in the dog ACL model in which Licofelone, a dual inhibitor of 5-lipoxygenase and COX activity, inhibited the development of cartilage lesions and subchondral bone resorption by reducing the synthesis of MMP-13 and cathepsin K, as well as other enzymes and growth factors that are involved in bone remodelling [93].

Negative_regulation (reducing) of Gene_expression (synthesis) of MMP-13 in cartilage associated with licofelone and hypercalcemia

17) Confidence 0.10 Published 2006 Journal Arthritis Res Ther Section Body Doc Link PMC1526599 Disease Relevance 0.74 Pain Relevance 0.23

The PPS salts are also potent inhibitors of granulocyte elastase and the serine proteinases of the complement and fibrinolytic systems, as well as downregulating MMP-13 production at the gene promotor level ([18-21] and references cited within).

Negative_regulation (downregulating) of Gene_expression (production) of MMP-13 in granulocyte

18) Confidence 0.07 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875662 Disease Relevance 0.29 Pain Relevance 0.19

It was recently reported that phenyl N-tert-butylnitrone, a spin-trap agent, downregulates IL-1-induced MMP-13 expression via the inhibition of the c-Jun amino-terminal kinase pathway in OA chondrocytes [61].

Negative_regulation (downregulates) of Gene_expression (expression) of MMP-13 in chondrocytes associated with osteoarthritis

19) Confidence 0.06 Published 2006 Journal Arthritis Res Ther Section Body Doc Link PMC1526599 Disease Relevance 0.86 Pain Relevance 0.33

Recent studies have revealed that pretreatment of human OA chondrocytes with EGCG significantly, in a dose-dependent manner, inhibited the expression and activities of MMP-1 and MMP-13 (IC50 values 27 and 16.5 ?

Negative_regulation (inhibited) of Gene_expression (expression) of MMP-13 in chondrocytes associated with osteoarthritis

20) Confidence 0.05 Published 2005 Journal Evidence-based Complementary and Alternative Medicine Section Body Doc Link PMC1193557 Disease Relevance 0.88 Pain Relevance 0.47

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