INT129963

Context Info Confidence 0.42 First Reported 2005 Last Reported 2008 Negated 2 Speculated 0 Reported most in Body Documents 8 Total Number 9 Disease Relevance 2.84 Pain Relevance 1.39

peptidase activity (MMP13) extracellular space (MMP13) extracellular region (MMP13) Golgi apparatus (MMP13) proteinaceous extracellular matrix (MMP13) lysosome (MMP13)

Anatomy Link Frequency chondrocytes 4 cartilage 2 MMP13 (Homo sapiens)

Pain Link Frequency Relevance Heat Osteoarthritis 201 98.38 cytokine 33 96.44 rheumatoid arthritis 100 93.28 metalloproteinase 46 90.00 Inflammation 95 78.44 Inflammatory response 2 74.36 Arthritis 39 51.04 agonist 2 50.00 COX2 2 14.88 Inflammatory mediators 5 14.68

Disease Link Frequency Relevance Heat Osteoarthritis 206 98.38 Arthritis 61 93.68 Rheumatoid Arthritis 102 93.28 Osteoporosis 8 91.72 Disease 42 79.48 INFLAMMATION 105 78.44 Degenerative Myopia 2 74.96 Conjunctiva Disease 2 73.16 Scleral Diseases 3 72.00 Chondrosarcoma 12 51.28

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

To determine whether this inhibition extended to the level of MMP protein production and enzymatic activity, we performed Western blot analysis of MMP-1 and MMP-13 protein levels in conditioned media and an in vitro collagen destruction assay looking at the breakdown of type I collagen matrix by IL-1?

Regulation (analysis) of Gene_expression (levels) of MMP-13

1) Confidence 0.42 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2656243 Disease Relevance 0 Pain Relevance 0

CONCLUSIONS: Fluoroquinolones show contrasting effects on the expression of the two collagenases MMP-1 and MMP-13, indicating specific effects on MMP gene regulation.

Regulation (effects) of Gene_expression (expression) of MMP-13

2) Confidence 0.41 Published 2005 Journal Rheumatology (Oxford) Section Body Doc Link 16148020 Disease Relevance 0 Pain Relevance 0

This suggests that these inhibitors are specific for the ET-1-activated pathways since they do not affect the basal levels of MMP-1 and MMP-13.

Neg (not) Regulation (affect) of Gene_expression (levels) of MMP-13

3) Confidence 0.25 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1065327 Disease Relevance 0.22 Pain Relevance 0.15

In this study, we address the inhibitory effects of adding both ligands on MMP-1 and MMP-13 expression in IL-1?

Regulation (effects) of Gene_expression (expression) of MMP-13

4) Confidence 0.25 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2656243 Disease Relevance 0.05 Pain Relevance 0.09

had no effect on the level of MMP-13 produced by Raw 264.7 cells (n = 8).

Regulation (effect) of Gene_expression (produced) of MMP-13

5) Confidence 0.22 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2483463 Disease Relevance 0.26 Pain Relevance 0.09

The expression of cartilage-associated genes, such as Indian hedge hog, type X collagen, and MMP13, was up-regulated within 3 weeks in vitro.

Regulation (regulated) of Gene_expression (expression) of MMP13 in cartilage

6) Confidence 0.17 Published 2008 Journal PLoS ONE Section Abstract Doc Link PMC2579486 Disease Relevance 0.40 Pain Relevance 0.05

In addition to the findings already discussed, the present study sheds more light on the major signalling pathways involved in the ET-1-induced MMP-1 and MMP-13 production and in NO production.

Neg (NO) Regulation (involved) of Gene_expression (production) of MMP-13

7) Confidence 0.15 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1065327 Disease Relevance 0.15 Pain Relevance 0.08

Recent studies have revealed that pretreatment of human OA chondrocytes with EGCG significantly, in a dose-dependent manner, inhibited the expression and activities of MMP-1 and MMP-13 (IC50 values 27 and 16.5 ?

Regulation (values) of Gene_expression (expression) of MMP-13 in chondrocytes associated with osteoarthritis

8) Confidence 0.05 Published 2005 Journal Evidence-based Complementary and Alternative Medicine Section Body Doc Link PMC1193557 Disease Relevance 0.88 Pain Relevance 0.47

Recent studies have revealed that pretreatment of human OA chondrocytes with EGCG significantly, in a dose-dependent manner, inhibited the expression and activities of MMP-1 and MMP-13 (IC50 values 27 and 16.5 ?

Regulation (activities) of Gene_expression (expression) of MMP-13 in chondrocytes associated with osteoarthritis

9) Confidence 0.05 Published 2005 Journal Evidence-based Complementary and Alternative Medicine Section Body Doc Link PMC1193557 Disease Relevance 0.88 Pain Relevance 0.47

General Comments

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