INT134470

From wiki-pain
Jump to: navigation, search
Context Info
Confidence 0.49
First Reported 2003
Last Reported 2010
Negated 3
Speculated 1
Reported most in Body
Documents 74
Total Number 79
Disease Relevance 46.07
Pain Relevance 3.02

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Golgi apparatus (Map2k1) cytoplasm (Map2k1) cytosol (Map2k1)
cell proliferation (Map2k1) mitosis (Map2k1) cell motility (Map2k1)
Anatomy Link Frequency
epithelial cells 14
IEC-6 6
liver 4
colon 4
fibroblasts 2
Map2k1 (Mus musculus)
Map2k1 - S218D (2)
Pain Link Frequency Relevance Heat
metalloproteinase 336 98.12 Very High Very High Very High
Inflammation 45 97.96 Very High Very High Very High
Inflammatory response 1 97.28 Very High Very High Very High
cytokine 52 96.76 Very High Very High Very High
imagery 34 95.68 Very High Very High Very High
rheumatoid arthritis 370 90.56 High High
Pain 6 88.04 High High
Morphine 1 88.00 High High
agonist 11 84.96 Quite High
Peripheral nervous system 1 84.00 Quite High
Disease Link Frequency Relevance Heat
Colon Cancer 1939 99.84 Very High Very High Very High
Targeted Disruption 194 99.84 Very High Very High Very High
Cancer 3497 99.76 Very High Very High Very High
Adenocarcinoma 346 99.36 Very High Very High Very High
Carcinoma 203 99.04 Very High Very High Very High
Hepatocellular Cancer 936 99.00 Very High Very High Very High
Malignant Neoplastic Disease 98 98.92 Very High Very High Very High
Apoptosis 1376 98.80 Very High Very High Very High
Coronary Heart Disease 6 98.60 Very High Very High Very High
Hyperplasia 112 98.56 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The results presented above clearly demonstrate that constitutive activation of either MEK isoform, MEK1 or MEK2, is sufficient to fully transform intestinal epithelial cells to the metastatic stage.
Positive_regulation (activation) of MEK1 in epithelial cells
1) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.62 Pain Relevance 0
In contrast, expression of activated MEK1 or MEK2 led to drastic morphological changes accompanied by loss of cell-cell contacts; the cells adopted a spindle-like fibroblast morphology, were more refractile and formed multilayers.
Positive_regulation (activated) of MEK1 in fibroblast
2) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.22 Pain Relevance 0
MEK1 or MEK2 activation had no significant effect on the expression of the pro-apoptotic proteins Bax and Bak in these cells (data not shown).


Positive_regulation (activation) of MEK1 associated with apoptosis
3) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.65 Pain Relevance 0
To assess the functional significance of MEK1/MEK2 activation in colorectal cancer, we ectopically expressed wild type and constitutively active (DD mutant) versions of MEK1 and MEK2 by retroviral gene transfer in the normal undifferentiated intestinal epithelial cell line IEC-6 [27].
Positive_regulation (activation) of MEK1 in IEC-6 associated with colon cancer
4) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.45 Pain Relevance 0
Constitutive activation of MEK1 or MEK2 resulted in the up-regulation of the pro-survival proteins Mcl-1, Bcl-2 and, to a lesser extent, Bcl-xL in IEC-6 cells (Fig. 5B).
Positive_regulation (activation) of MEK1 in IEC-6
5) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.83 Pain Relevance 0
No major difference was observed in the growth rate of tumors expressing activated MEK1 or MEK2 (Fig. 2D).
Positive_regulation (activated) of MEK1 associated with cancer
6) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.57 Pain Relevance 0
Here, we clearly establish that expression of activated MEK1 is sufficient to morphologically transform intestinal epithelial cells, accelerate cell proliferation, and induce the rapid formation of aggressive tumors after orthotopic transplantation.
Positive_regulation (activated) of MEK1 in epithelial cells associated with cancer
7) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.54 Pain Relevance 0
Constitutive activation of MEK1 or MEK2 confers metastatic properties to transformed intestinal epithelial cells
Positive_regulation (activation) of MEK1 in epithelial cells
8) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.79 Pain Relevance 0
Both activated MEK1 and MEK2 conferred anchorage-independence growth to IEC-6 cells (Fig. 2B).
Positive_regulation (activated) of MEK1 in IEC-6
9) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.27 Pain Relevance 0
We examined the impact of MEK1 or MEK2 activation on the motility of IEC-6 cells using two different cell migration assays.
Spec (examined) Positive_regulation (activation) of MEK1 in IEC-6
10) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.68 Pain Relevance 0
These observations indicate that constitutive activation of either MEK1 or MEK2 is sufficient to confer a metastatic phenotype to intestinal tumor cells.
Positive_regulation (activation) of MEK1 associated with intestinal cancer
11) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.52 Pain Relevance 0
Constitutive activation of MEK1 or MEK2 protects intestinal epithelial cells against anoikis
Positive_regulation (activation) of MEK1 in epithelial cells
12) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.62 Pain Relevance 0.04
Here, we show that expression of activated MEK1 or MEK2 not only induces the formation of intestinal tumors but also promotes later stages of tumor progression and metastasis to distant organs.
Positive_regulation (activated) of MEK1 associated with cancer, intestinal cancer and metastasis
13) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 1.51 Pain Relevance 0
Quantitative PCR analysis confirmed that constitutive activation of MEK1 or MEK2 induces the expression of urokinase receptor mRNA (Fig. 4B).
Positive_regulation (activation) of MEK1
14) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.07 Pain Relevance 0.18
Several human colon carcinoma cell lines display constitutive phosphorylation of ERK1/ERK2 MAP kinases [20], likely resulting from activation of MEK1/MEK2.
Positive_regulation (activation) of MEK1 in colon associated with colon cancer
15) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.50 Pain Relevance 0
As expected, substitution of the activation loop Ser phosphorylation sites by Asp residues strongly potentiated the enzymatic activity of MEK1 and MEK2, but no reproducible difference in activity was observed between the two isoforms (Fig. 1C).
Positive_regulation (potentiated) of MEK1
16) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.37 Pain Relevance 0
Together, these data demonstrate that constitutive activation of MEK1 or MEK2 is sufficient to transform intestinal epithelial cells and induce the formation of invasive colon adenocarcinomas.


Positive_regulation (activation) of MEK1 in colon associated with adenocarcinoma
17) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.93 Pain Relevance 0
However, activation of either MEK1 or MEK2 markedly up-regulated the expression of MMP-13 protein (Fig. 4A).
Positive_regulation (activation) of MEK1
18) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.20 Pain Relevance 0.20
To further extend our investigation to non-colorectal carcinomas, we tested the effect of MEK1 and MEK2 shRNAs on the human breast adenocarcinoma cell line MDA-MB-231, which exhibit strong constitutive activation of MEK1/MEK2 signaling (Fig. 6A).
Positive_regulation (activation) of MEK1 in MDA-MB-231 associated with adenocarcinoma and colon cancer
19) Confidence 0.49 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.34 Pain Relevance 0
Here, we report that Cdk5 regulates mitogen-activated protein kinase kinase1/2 (MEK1/2) activity through a negative feedback loop during the peripheral inflammatory response.
Positive_regulation (mitogen-activated) of MEK1 associated with inflammatory response
20) Confidence 0.49 Published 2006 Journal Cell Cycle Section Abstract Doc Link 16552189 Disease Relevance 0.88 Pain Relevance 0.71

General Comments

This test has worked.

Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox