INT134596
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Given the broad range of molecules that can affect TRPA1 activity, a variety of possibilities, including local increases in intracellular calcium concentration [19,32,33], could explain how TRPA1 responds to activity of a mechanoreceptor. | |||||||||||||||
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In this study, we selected miogadial (MD), miogatrial (MT), and polygodial (PG) from the terpenoids with an alpha,beta-unsaturated 1,4-dialdehyde moiety and examined the effects of these 3 terpenoids on TRPA1 or TRPV1. | |||||||||||||||
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In order to study the regulation of TRPA1 mRNA in another model, we also examined its expression during inflammatory pain. | |||||||||||||||
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However, we and others [39] detected no up-regulation of TRPA1 in either injured or uninjured neurons after nerve injury, but were still able to measure robust cold allodynia. | |||||||||||||||
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Cisplatin induced significant up-regulation of TRPV1 and TRPA1 mRNA (3-fold) in cultured DRG neurons at 6 h (Figure 2A and 2C). | |||||||||||||||
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We also illustrate that up-regulation of TRPV1 and TRPA1 mRNA reflects increases in TRPV1 and TRPA1 responsiveness in the nociceptors that contribute to the molecular mechanisms of the thermal hyperalgesia and mechanical allodynia observed in cisplatin-treated mice. | |||||||||||||||
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While our results illustrate that changes in the relative levels of TRPV1 and TRPA1 mRNA in vivo were similar to those observed in vitro (Figure 2), the increase in TRPM8 mRNA that was observed in oxaliplatin-treated mice was less than that seen in vitro. | |||||||||||||||
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Overall, the regulation of Trpa1 did not correlate significantly with any of the pain parameters considered. | |||||||||||||||
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More importantly, we show that the shRNA and miRNA sequences targeting trpV1 have no effect on the expression levels of the closely related trpA1 gene or stat1, which is another gene involved in the INF response, when expressed in neurons in vivo. | |||||||||||||||
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TRPA1 antagonist alters mechanically-evoked firing in mouse C fibers, but not in TRPA1-deficient littermates | |||||||||||||||
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The effects of TRPV4 and TRPA1 agonists on [Ca(2+)](i), pain and inflammation were markedly diminished or abolished in trpv4 and trpa1 knockout mice. | |||||||||||||||
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The present study focused on the effects of TRPA1 activation on contractile responses in isolated mouse intestine preparations. | |||||||||||||||
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Menthol-induced cold hypersensitivity is dependent on TRPA1 but not TRPM8 | |||||||||||||||
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However, using the same model, another study reported down-regulation of both TRPA1 and TRPM8 in injured neurons and no change in expression in the uninjured ganglion [39]. | |||||||||||||||
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Intriguingly the reduction in TRPA1 expression was seen exclusively in the IB4-positive neurons, with no change in the number of TRPA1 plus CGRP or TRPA1 plus NF200 positive cells (Table 2). | |||||||||||||||
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First, we demonstrate that treatment with platinum drugs results in the up-regulation of TRPV1, TRPA1, and TRPM8 mRNA in cultured dorsal root ganglion (DRG) neurons and that a similar up-regulation occurs with TRPV1 and TRPA1 following in vivo treatment with cisplatin although cisplatin-treated mice had no change in the proportion of TRPV1-immunopositive TG neurons. | |||||||||||||||
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Cisplatin and oxaliplatin induced up-regulation of TRPV1, TRPM8 and TRPA1 mRNA in vitro | |||||||||||||||
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Oxaliplatin treatment results in up-regulation of only TRPA1 in vivo. | |||||||||||||||
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The most parsimonious explanation of these results is that NO's behavioral consequences is due to direct activation of these thermoTRPs; however, it cannot be ruled out that NO also targets TRPV1 and TRPA1 in vivo through indirect mechanisms. | |||||||||||||||
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Regardless, BH4-induced intracellular calcium increase in primary cultured DRG neurons is partly dependent on NOS [19], and essentially dependent on TRPV1 and TRPA1 (Fig. 5). | |||||||||||||||
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General Comments
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