INT134874

Context Info Confidence 0.58 First Reported 2006 Last Reported 2009 Negated 0 Speculated 0 Reported most in Body Documents 3 Total Number 5 Disease Relevance 0.28 Pain Relevance 2.12

plasma membrane (Dlg4) protein complex assembly (Dlg4) response to stress (Dlg4) cytoplasm (Dlg4)

Anatomy Link Frequency neuronal 2 neurons 1 synapse 1 Dlg4 (Rattus norvegicus)

Pain Link Frequency Relevance Heat Morphine 22 100.00 addiction 15 100.00 Neuronal nitric oxide synthase 2 96.52 Central nervous system 11 94.32 tetrodotoxin 48 93.88 withdrawal 2 91.08 Dextromethorphan 6 79.76 Hippocampus 2 75.00 imagery 60 41.00 depression 6 40.80

Disease Link Frequency Relevance Heat Pathologic Processes 6 94.20 Opiate Addiction 2 91.44 Depression 6 40.80 Increased Venous Pressure Under Development 3 5.00 Nervous System Malformation 3 5.00 Stress 3 5.00 Infection 3 5.00

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Anatomy

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Here we studied the effects of prenatal morphine exposure on postsynaptic density protein 95 (PSD-95), an important cytoskeletal specialization involved in the anchoring of the NMDAR and neuronal nitric oxide synthase (nNOS), of the hippocampal CA1 subregion from young offspring at postnatal day 14 (P14).

Regulation (effects) of PSD-95 in neuronal associated with neuronal nitric oxide synthase and morphine

1) Confidence 0.58 Published 2006 Journal Hippocampus Section Abstract Doc Link 16598705 Disease Relevance 0.09 Pain Relevance 0.83

Here we studied the effects of prenatal morphine exposure on postsynaptic density protein 95 (PSD-95), an important cytoskeletal specialization involved in the anchoring of the NMDAR and neuronal nitric oxide synthase (nNOS), of the hippocampal CA1 subregion from young offspring at postnatal day 14 (P14).

Regulation (effects) of postsynaptic density protein 95 in neuronal associated with neuronal nitric oxide synthase and morphine

2) Confidence 0.58 Published 2006 Journal Hippocampus Section Abstract Doc Link 16598705 Disease Relevance 0.09 Pain Relevance 0.83

In the aforementioned experiments, we observed significant changes in PSD-95:GFP puncta number and fluorescence intensity, which, in all likelihood, reflected changes in glutamatergic synapse number and PSD size.

Regulation (changes) of PSD-95 in synapse

3) Confidence 0.53 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2693930 Disease Relevance 0.09 Pain Relevance 0

Furthermore, this was seen for both the entire PSD-95:GFP population and for the smaller population of tracked PSD-95:GFP puncta (Figure S5B), indicating that the population of tracked puncta faithfully represented the entire PSD-95:GFP puncta population.

Regulation (population) of PSD-95

4) Confidence 0.44 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2693930 Disease Relevance 0 Pain Relevance 0.27

Despite the reduction in activity levels, changes in puncta fluorescence still occurred to similar degrees, although the dependence of such changes on initial PSD-95:GFP puncta fluorescence was reduced or lost, in particular at higher diazepam concentrations (five experiments, tracked synapse data from four neurons).

Regulation (dependence) of PSD-95 in neurons associated with addiction

5) Confidence 0.39 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2693930 Disease Relevance 0 Pain Relevance 0.18

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