INT134914

Context Info Confidence 0.37 First Reported 2004 Last Reported 2011 Negated 2 Speculated 1 Reported most in Body Documents 21 Total Number 22 Disease Relevance 8.25 Pain Relevance 3.67

structural molecule activity (Gfap) extracellular matrix organization (Gfap) cytoplasm (Gfap)

Anatomy Link Frequency astrocytes 6 dorsal root ganglion 2 neuronal 1 glial cell 1 neuron 1 Gfap (Mus musculus)

Pain Link Frequency Relevance Heat dorsal root ganglion 2 98.54 spinal dorsal horn 1 97.96 Neuritis 1 97.52 Dorsal horn 49 97.48 Spinal cord 123 97.42 Inflammation 248 96.88 cINOD 26 94.88 cerebral cortex 56 94.16 intrathecal 37 93.68 Sciatic nerve 50 93.04

Disease Link Frequency Relevance Heat Disease 496 99.12 Amyloid Plaque 39 99.10 Ganglion Cysts 27 98.54 Injury 33 97.52 Neuritis 1 97.52 Gliosis 21 96.92 INFLAMMATION 280 96.88 Targeted Disruption 522 96.48 Congenital Anomalies 54 96.16 Pain 81 91.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Furthermore, 5-bromo-2'-deoxyuridine (BrdU)-positive cells on the ipsilateral dorsal horn of the spinal cord were significantly increased at 7 days after nerve ligation and were highly co-localized with another microglia marker, ionized calcium-binding adaptor molecule 1 (Iba1), but neither with GFAP nor a specific neural nuclei marker, NeuN, in the spinal dorsal horn of nerve-ligated mice.

GFAP Neg (neither) Binding (calcium-binding) of in nerve associated with spinal dorsal horn, dorsal horn and spinal cord

1) Confidence 0.37 Published 2006 Journal J. Neurochem. Section Abstract Doc Link 16606373 Disease Relevance 0.32 Pain Relevance 0.86

The MHCI-HC signal in the V1 did not colocalize with GFAP-positive astrocytes (Additional file 4).

GFAP Neg (not) Binding (colocalize) of in astrocytes

2) Confidence 0.36 Published 2011 Journal Behav Brain Funct Section Body Doc Link PMC3023691 Disease Relevance 0 Pain Relevance 0

Such neuronal cell loss was suggested by the brain MRI studies and the increase of GFAP message in the full genome microarrays which reflected neuronal cell injury (see below).

GFAP Binding (message) of in neuronal associated with injury

3) Confidence 0.32 Published 2008 Journal J Neuroinflammation Section Body Doc Link PMC2588578 Disease Relevance 0.42 Pain Relevance 0

Antibodies specific to glial fibrillary acidic protein (GFAP) (1:1000, Sigma-Aldrich USA, St.

glial fibrillary acidic protein Binding (1:1000) of

4) Confidence 0.31 Published 2010 Journal Mol Brain Section Body Doc Link PMC2873589 Disease Relevance 0 Pain Relevance 0.05

Antibodies specific to glial fibrillary acidic protein (GFAP) (1:1000, Sigma-Aldrich USA, St.

GFAP Binding (1:1000) of

5) Confidence 0.31 Published 2010 Journal Mol Brain Section Body Doc Link PMC2873589 Disease Relevance 0 Pain Relevance 0.04

Quantification showed that pIONL significantly increased the intensity of GFAP-IR in ipsilateral TREZ in WT mice compared with sham operated mice or with the contralateral TREZ (Fig. 4A, B, C, F).

GFAP-IR Binding (intensity) of

6) Confidence 0.31 Published 2010 Journal Mol Pain Section Body Doc Link PMC2826348 Disease Relevance 0.20 Pain Relevance 0.47

Tg2576 mice display age-related neocortical and hippocampal amyloid deposits, which correlate with microglia activation, reactive astrocytes with increased GFAP, IL-1?

GFAP Binding (reactive) of in astrocytes associated with amyloid plaque

7) Confidence 0.31 Published 2004 Journal J Neuroinflammation Section Body Doc Link PMC527877 Disease Relevance 1.48 Pain Relevance 0.53

Remarkebly, activated microglial and astrocytic cells were colocalized as demonstrated by double staining for CD11b and GFAP, already in the brain of young APP [V717I] mice, suggesting the formation of inflammatory foci in both brain regions evaluated (not shown).

GFAP Binding (staining) of in brain associated with inflammation

8) Confidence 0.30 Published 2005 Journal J Neuroinflammation Section Body Doc Link PMC1274341 Disease Relevance 0.76 Pain Relevance 0.26

The axonal length that colocalized with GFAP (Y) was then measured; and the proportion of the branch that colocolized with GFAP was obtained as Y / X * 100.

GFAP Binding (colocolized) of

9) Confidence 0.29 Published 2008 Journal PLoS Biology Section Body Doc Link PMC2689695 Disease Relevance 0 Pain Relevance 0

Immunoreactivity for CD11b and GFAP, markers for activated microglia and astrocyte, respectively, increased in 13–15 month-old TLR4m AD mice compared to TLR4w AD mice.

GFAP Binding (Immunoreactivity) of in microglia associated with disease

10) Confidence 0.29 Published 2008 Journal J Neuroinflammation Section Body Doc Link PMC2430555 Disease Relevance 1.16 Pain Relevance 0.10

We did not observe a complete overlap between eGFP and GFAP.

GFAP Binding (overlap) of

11) Confidence 0.28 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2888059 Disease Relevance 0.13 Pain Relevance 0

Astrocyte immunoreactivity for GFAP in the cortex of non-AD mice was unremarkable (data not shown).

GFAP Binding (immunoreactivity) of in cortex associated with disease

12) Confidence 0.28 Published 2008 Journal J Neuroinflammation Section Body Doc Link PMC2430555 Disease Relevance 1.35 Pain Relevance 0.19

Our GFAP labeling of BG likely underestimated their association with stellate axons.

GFAP Binding (association) of

13) Confidence 0.25 Published 2008 Journal PLoS Biology Section Body Doc Link PMC2689695 Disease Relevance 0.08 Pain Relevance 0.08

Although their axons extended in different directions, many of their descending/ascending branches were strictly associated with GFAP-labeled BG fibers (Figure 2E).

GFAP Binding (associated) of

14) Confidence 0.25 Published 2008 Journal PLoS Biology Section Body Doc Link PMC2689695 Disease Relevance 0.05 Pain Relevance 0

Anti-CD31 was used to stain the endothelial cells of the vasculature, whereas anti-ionized calcium binding adapter molecule 1 (IBA1), anti-glial fibrillary acidic protein (GFAP) and anti-neuron specific nuclear protein (NEUN) labeled cells of myeloid (macrophages and microglia), astroglial and neuronal lineages, respectively.

GFAP Binding (binding) of in neuron

15) Confidence 0.24 Published 2007 Journal PLoS ONE Section Body Doc Link PMC1819560 Disease Relevance 0 Pain Relevance 0

Tyrosine hydroxylase (TH), glial cell line-derived neurotrophic factor (GDNF) and glial fibrillary acidic protein (GFAP) immunostaining

GFAP Binding (line) of in glial cell

16) Confidence 0.20 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2719807 Disease Relevance 0 Pain Relevance 0.05

No colocalization was observed with glial cell markers glial fibrillary acidic protein (GFAP; astrocytes) nor ionized calcium binding adaptor molecule 1 (Iba1; microglia).

fibrillary acidic protein Binding (binding) of in astrocytes

17) Confidence 0.16 Published 2009 Journal Mol Pain Section Body Doc Link PMC2747840 Disease Relevance 0.16 Pain Relevance 0.70

Conversely, no co-localization was found with cortical astrocytes, as shown by labeling with the specific markers GFAP (Figure 2E) and S100?

GFAP Binding (labeling) of in astrocytes

18) Confidence 0.14 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2570486 Disease Relevance 0 Pain Relevance 0.04

Immunoreactivity for CD11b and GFAP, markers for activated microglia and astrocyte, respectively, increased in 13–15 month-old TLR4m AD mice compared to TLR4w AD mice.

GFAP Binding (Immunoreactivity) of in astrocyte associated with disease

19) Confidence 0.10 Published 2008 Journal J Neuroinflammation Section Body Doc Link PMC2430555 Disease Relevance 1.16 Pain Relevance 0.10

Cultures consisted of >98% astrocytes as determined by staining for glial fibrillary acidic protein (GFAP).

GFAP Spec (determined) Binding (staining) of in astrocytes

20) Confidence 0.08 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2605563 Disease Relevance 0.15 Pain Relevance 0

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