INT134914
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Furthermore, 5-bromo-2'-deoxyuridine (BrdU)-positive cells on the ipsilateral dorsal horn of the spinal cord were significantly increased at 7 days after nerve ligation and were highly co-localized with another microglia marker, ionized calcium-binding adaptor molecule 1 (Iba1), but neither with GFAP nor a specific neural nuclei marker, NeuN, in the spinal dorsal horn of nerve-ligated mice. | |||||||||||||||
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The MHCI-HC signal in the V1 did not colocalize with GFAP-positive astrocytes (Additional file 4). | |||||||||||||||
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Such neuronal cell loss was suggested by the brain MRI studies and the increase of GFAP message in the full genome microarrays which reflected neuronal cell injury (see below). | |||||||||||||||
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Antibodies specific to glial fibrillary acidic protein (GFAP) (1:1000, Sigma-Aldrich USA, St. | |||||||||||||||
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Antibodies specific to glial fibrillary acidic protein (GFAP) (1:1000, Sigma-Aldrich USA, St. | |||||||||||||||
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Quantification showed that pIONL significantly increased the intensity of GFAP-IR in ipsilateral TREZ in WT mice compared with sham operated mice or with the contralateral TREZ (Fig. 4A, B, C, F). | |||||||||||||||
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Tg2576 mice display age-related neocortical and hippocampal amyloid deposits, which correlate with microglia activation, reactive astrocytes with increased GFAP, IL-1? | |||||||||||||||
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Remarkebly, activated microglial and astrocytic cells were colocalized as demonstrated by double staining for CD11b and GFAP, already in the brain of young APP [V717I] mice, suggesting the formation of inflammatory foci in both brain regions evaluated (not shown). | |||||||||||||||
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The axonal length that colocalized with GFAP (Y) was then measured; and the proportion of the branch that colocolized with GFAP was obtained as Y / X * 100. | |||||||||||||||
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Immunoreactivity for CD11b and GFAP, markers for activated microglia and astrocyte, respectively, increased in 1315 month-old TLR4m AD mice compared to TLR4w AD mice. | |||||||||||||||
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We did not observe a complete overlap between eGFP and GFAP. | |||||||||||||||
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Astrocyte immunoreactivity for GFAP in the cortex of non-AD mice was unremarkable (data not shown). | |||||||||||||||
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Our GFAP labeling of BG likely underestimated their association with stellate axons. | |||||||||||||||
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Although their axons extended in different directions, many of their descending/ascending branches were strictly associated with GFAP-labeled BG fibers (Figure 2E). | |||||||||||||||
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Anti-CD31 was used to stain the endothelial cells of the vasculature, whereas anti-ionized calcium binding adapter molecule 1 (IBA1), anti-glial fibrillary acidic protein (GFAP) and anti-neuron specific nuclear protein (NEUN) labeled cells of myeloid (macrophages and microglia), astroglial and neuronal lineages, respectively. | |||||||||||||||
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Tyrosine hydroxylase (TH), glial cell line-derived neurotrophic factor (GDNF)
and glial fibrillary acidic protein (GFAP) immunostaining
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No colocalization was observed with glial cell markers glial fibrillary acidic protein (GFAP; astrocytes) nor ionized calcium binding adaptor molecule 1 (Iba1; microglia). | |||||||||||||||
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Conversely, no co-localization was found with cortical astrocytes, as shown by labeling with the specific markers GFAP (Figure 2E) and S100? | |||||||||||||||
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Immunoreactivity for CD11b and GFAP, markers for activated microglia and astrocyte, respectively, increased in 1315 month-old TLR4m AD mice compared to TLR4w AD mice. | |||||||||||||||
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Cultures consisted of >98% astrocytes as determined by staining for glial fibrillary acidic protein (GFAP). | |||||||||||||||
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