INT134916

Context Info Confidence 0.73 First Reported 2006 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 3 Total Number 3 Disease Relevance 1.58 Pain Relevance 2.00

cytosol (Aif1) plasma membrane (Aif1) cytoskeleton (Aif1) cytoplasm (Aif1)

Anatomy Link Frequency glial cell 1 spinal cord 1 nerve 1 Aif1 (Mus musculus)

Pain Link Frequency Relevance Heat Dorsal horn 12 99.80 Spinal cord 55 99.32 spinal dorsal horn 1 97.00 intrathecal 30 96.00 Sciatic nerve 31 95.36 Lasting pain 14 93.32 Thermal hyperalgesia 7 91.96 Neuropathic pain 72 81.92 Brush evoked pain 13 76.48 superficial lamina 3 74.20

Disease Link Frequency Relevance Heat Diabetes Mellitus 154 95.68 Pain 43 93.32 Hyperalgesia 10 91.96 Neuropathic Pain 94 81.92 Hypersensitivity 11 75.64 Nociception 32 70.28 Peripheral Neuropathy 26 70.28 Sleep Disorders 3 30.88 Nervous System Injury 20 5.00 INFLAMMATION 18 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Furthermore, 5-bromo-2'-deoxyuridine (BrdU)-positive cells on the ipsilateral dorsal horn of the spinal cord were significantly increased at 7 days after nerve ligation and were highly co-localized with another microglia marker, ionized calcium-binding adaptor molecule 1 (Iba1), but neither with GFAP nor a specific neural nuclei marker, NeuN, in the spinal dorsal horn of nerve-ligated mice.

Localization (co-localized) of Iba1 in nerve associated with spinal dorsal horn, dorsal horn and spinal cord

1) Confidence 0.73 Published 2006 Journal J. Neurochem. Section Abstract Doc Link 16606373 Disease Relevance 0.32 Pain Relevance 0.86

Western blotting confirmed lower levels of both Iba-1 and p-p38 MAPK protein expression in the dorsal spinal cord (Figure 8) of mice who received cannabidiol at diabetes onset.

Localization (levels) of Iba-1 in spinal cord associated with diabetes mellitus and spinal cord

2) Confidence 0.23 Published 2010 Journal Mol Pain Section Body Doc Link PMC2845559 Disease Relevance 1.10 Pain Relevance 0.42

No colocalization was observed with glial cell markers glial fibrillary acidic protein (GFAP; astrocytes) nor ionized calcium binding adaptor molecule 1 (Iba1; microglia).

Localization (colocalization) of Iba1 in glial cell

3) Confidence 0.21 Published 2009 Journal Mol Pain Section Body Doc Link PMC2747840 Disease Relevance 0.16 Pain Relevance 0.72

General Comments

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