INT135187

Context Info Confidence 0.70 First Reported 2006 Last Reported 2009 Negated 0 Speculated 0 Reported most in Body Documents 9 Total Number 10 Disease Relevance 3.53 Pain Relevance 1.87

signal transduction (TNFRSF11B) extracellular region (TNFRSF11B) proteinaceous extracellular matrix (TNFRSF11B) extracellular matrix organization (TNFRSF11B)

Anatomy Link Frequency neutrophils 2 blood 1 osteoblasts 1 TNFRSF11B (Homo sapiens)

Pain Link Frequency Relevance Heat rheumatoid arthritis 291 99.98 Osteoarthritis 183 99.18 cytokine 68 90.20 Bioavailability 2 77.36 Inflammation 166 54.84 COX2 4 10.00 Arthritis 22 5.00 cINOD 7 5.00 Pain 5 5.00 psoriasis 5 5.00

Disease Link Frequency Relevance Heat Rheumatoid Arthritis 291 99.98 Osteoarthritis 183 99.18 Apoptosis 42 91.60 Osteoporosis 49 87.04 INFLAMMATION 163 54.84 Hypercalcemia 26 26.92 Necrosis 49 5.00 Cancer 49 5.00 Disease 24 5.00 Arthritis 17 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Effects on cellular oxygen consumption were measured amperometrically using a Clark electrode. mRNA expression of GLUT-1 and OPG was determined by RT-PCR; OPG protein secretion by ELISA and HIF-1alpha protein expression by immunoblotting.

Transcription (expression) of OPG

1) Confidence 0.70 Published 2009 Journal Clin. Exp. Rheumatol. Section Body Doc Link 19327236 Disease Relevance 0.09 Pain Relevance 0

Freshly isolated neutrophils from SF of patients with RA expressed not only the mRNA of the four genes studied (Figure 1a) but also the corresponding proteins RANK-L, OPG, and RANK (Figure 2).

Transcription (expressed) of OPG in neutrophils associated with rheumatoid arthritis

2) Confidence 0.61 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC1906801 Disease Relevance 0.55 Pain Relevance 0.28

On the OPG and RANKL system, our data revealed that CS can modulate the expression of these molecules by increasing OPG and decreasing the gene expression level of RANKL, thereby increasing the mRNA ratio of OPG/RANKL.

Transcription (ratio) of OPG

3) Confidence 0.59 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC2246236 Disease Relevance 0.06 Pain Relevance 0.07

g/mL), or both together on human OA subchondral bone osteoblasts, in the presence or absence of 1,25(OH)2D3 (vitamin D3) (50 nM), was determined on the bone biomarkers alkaline phosphatase and osteocalcin, on the expression (mRNA) and production (enzyme-linked immunosorbent assay) of bone remodelling factors OPG and RANKL, and on the pro-resorptive activity of these cells.

Transcription (expression) of OPG in osteoblasts associated with osteoarthritis

4) Confidence 0.59 Published 2007 Journal Arthritis Res Ther Section Abstract Doc Link PMC2246236 Disease Relevance 0.65 Pain Relevance 0.29

The mRNA of RANKL, osteoprotegerin (OPG) and cyclooxygenase-2 (COX-2) were measured by semiquantitative reverse transcription-polymerase chain reaction.

Transcription (mRNA) of OPG

5) Confidence 0.52 Published 2006 Journal Pharmacology Section Body Doc Link 16636611 Disease Relevance 0 Pain Relevance 0

The expression of OPG mRNA, which was absent at day 0 (Figure 1a), significantly increased at days 1, 2, and 3 (Figure 3).

Transcription (expression) of OPG

6) Confidence 0.47 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC1906801 Disease Relevance 0.35 Pain Relevance 0.22

The expression of OPG mRNA, which was absent at day 0 (Figure 1a), significantly increased at days 1, 2, and 3 (Figure 3).

Transcription (absent) of OPG

7) Confidence 0.47 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC1906801 Disease Relevance 0.35 Pain Relevance 0.22

The expression of OPG mRNA by neutrophils incubated in CM was not yet detectable on day 2 and appeared only after 3 days (Figure 3).

Transcription (expression) of OPG in neutrophils

8) Confidence 0.47 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC1906801 Disease Relevance 0.45 Pain Relevance 0.26

Moreover, SF from patients with RA, but not patients with OA, activated healthy blood neutrophils to express OPG and RANK mRNAs as evaluated by RT-PCR (Figure 4b).

Transcription (express) of OPG in blood associated with rheumatoid arthritis and osteoarthritis

9) Confidence 0.41 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC1906801 Disease Relevance 1.03 Pain Relevance 0.54

The mRNA of RANKL, osteoprotegerin (OPG) and cyclooxygenase-2 (COX-2) were measured by semiquantitative reverse transcription-polymerase chain reaction.

Transcription (mRNA) of osteoprotegerin

10) Confidence 0.37 Published 2006 Journal Pharmacology Section Body Doc Link 16636611 Disease Relevance 0 Pain Relevance 0

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