INT136176

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Context Info
Confidence 0.60
First Reported 2006
Last Reported 2010
Negated 2
Speculated 1
Reported most in Body
Documents 5
Total Number 10
Disease Relevance 3.18
Pain Relevance 2.64

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

signal transduction (P2rx4) transport (P2rx4) plasma membrane (P2rx4)
Anatomy Link Frequency
plasma 6
microglia 6
C8-B4 4
spinal cord 2
cortex 2
P2rx4 (Mus musculus)
Pain Link Frequency Relevance Heat
Neuropathic pain 70 99.60 Very High Very High Very High
Spinal cord 9 99.56 Very High Very High Very High
tetrodotoxin 1 99.36 Very High Very High Very High
sodium channel 1 98.52 Very High Very High Very High
antagonist 38 97.76 Very High Very High Very High
IPN 5 97.04 Very High Very High Very High
Inflammation 12 95.56 Very High Very High Very High
gABA 7 95.04 Very High Very High Very High
Glutamate 7 93.88 High High
Peripheral nerve injury 3 93.68 High High
Disease Link Frequency Relevance Heat
Neuropathic Pain 109 99.60 Very High Very High Very High
Injury 7 98.52 Very High Very High Very High
Inflammatory Pain 5 97.04 Very High Very High Very High
Hypertrophy 1 95.60 Very High Very High Very High
INFLAMMATION 12 95.56 Very High Very High Very High
Nervous System Injury 22 93.68 High High
Targeted Disruption 6 92.00 High High
Infection 1 90.20 High High
Disease 1 89.76 High High
Pain 41 86.84 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Cognizant of the aforementioned limitations of using immunocytochemistry, we used surface biotinylation of membrane proteins to obtain more direct data to support our hypothesis that C8-B4 cell activation results in elevated P2X4 expression in the plasma membrane.
Positive_regulation (results) of Gene_expression (expression) of P2X4 in plasma
1) Confidence 0.60 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0
Resting C8-B4 cells expressed virtually no functional P2X receptors, but largely increased functional expression of P2X4 receptors within 2–6 h of entering the activated state.
Neg (no) Positive_regulation (increased) of Gene_expression (expression) of P2X4 in C8-B4
2) Confidence 0.60 Published 2010 Journal The Journal of General Physiology Section Abstract Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0
These results indicate functional expression of P2X4 receptors in cortical neurones and their involvement in purinergic synaptic transmission in cortex.
Positive_regulation (indicate) of Gene_expression (expression) of P2X4 in cortex
3) Confidence 0.49 Published 2007 Journal Neurosci. Lett. Section Abstract Doc Link 17566648 Disease Relevance 0 Pain Relevance 0.38
In contrast to the neuropathic pain model, neither the upregulation of P2X4R expression [7] nor activation of Lyn tyrosine kinase [13] has been demonstrated in spinal microglia following peripheral inflammation caused by CFA.
Neg (neither) Positive_regulation (upregulation) of Gene_expression (expression) of P2X4R in microglia associated with inflammation and neuropathic pain
4) Confidence 0.47 Published 2009 Journal Mol Pain Section Body Doc Link PMC2704200 Disease Relevance 1.39 Pain Relevance 0.98
This limitation is increased by the fact that available P2X4 antibodies target a cytosolic domain epitope and require cell permeabilization, rendering the increase in plasma membrane expression of P2X4 difficult to detect against a much larger cytosolic component.
Positive_regulation (increase) of Gene_expression (expression) of P2X4 in plasma
5) Confidence 0.43 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0
To explore this possibility, we used surface biotinylation and found increased levels of P2X4 protein in the membranes of activated C8-B4 cells, which may explain the increase in peak currents observed during C8-B4 cell activation (Table I) or when lysosomal secretion was triggered by MA (Qureshi et al., 2007).
Positive_regulation (increased) of Gene_expression (levels) of P2X4 protein in C8-B4
6) Confidence 0.40 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0.06 Pain Relevance 0.12
Cognizant of the aforementioned limitations of using immunocytochemistry, we used surface biotinylation of membrane proteins to obtain more direct data to support our hypothesis that C8-B4 cell activation results in elevated P2X4 expression in the plasma membrane.
Positive_regulation (elevated) of Gene_expression (expression) of P2X4 in plasma
7) Confidence 0.40 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0
In a later section, we examine surface P2X4 expression in resting and activated microglia using surface biotinylation.


Spec (examine) Positive_regulation (surface) of Spec (examine) Gene_expression (expression) of P2X4 in microglia
8) Confidence 0.38 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0.09 Pain Relevance 0
After injury to a peripheral nerve, microglia in the spinal cord become activated and upregulate expression of the P2X4 receptor.
Positive_regulation (upregulate) of Gene_expression (expression) of P2X4 receptor in spinal cord associated with injury and spinal cord
9) Confidence 0.18 Published 2006 Journal Pflugers Arch. Section Abstract Doc Link 16767466 Disease Relevance 0.88 Pain Relevance 0.50
These activated microglia upregulate expression of P2X/Y receptors (e.g., P2X4 and P2Y12).
Positive_regulation (upregulate) of Gene_expression (expression) of P2X4 in microglia
10) Confidence 0.15 Published 2010 Journal Brain Res Rev Section Abstract Doc Link 19931560 Disease Relevance 0.76 Pain Relevance 0.66

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