INT139150

Context Info Confidence 0.35 First Reported 2006 Last Reported 2010 Negated 1 Speculated 0 Reported most in Body Documents 4 Total Number 4 Disease Relevance 0.28 Pain Relevance 0.98

plasma membrane (Psd) intracellular (Psd) cytoplasm (Psd)

Anatomy Link Frequency astrocyte 1 synapses 1 hippocampus 1 Psd (Mus musculus)

Pain Link Frequency Relevance Heat Glutamate receptor 7 100.00 Morphine 7 99.36 Hippocampus 12 98.54 Glutamate 48 88.20 long-term potentiation 29 87.84 amygdala 2 75.08 Somatosensory cortex 3 61.16 nMDA receptor 10 58.48 Central nervous system 12 56.64 gABA 9 50.00

Disease Link Frequency Relevance Heat Targeted Disruption 10 95.20 Pheochromocytoma 16 51.32 Hypertrophy 2 50.96 Ganglion Cysts 4 22.80 Body Weight 4 5.00 Toxicity 2 5.00 Death 2 5.00 Generalized Anxiety Disorder 2 5.00 Epilepsy 2 5.00 Drug Induced Neurotoxicity 2 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

We applied a quantitative proteomics analysis technique with the cleavable ICAT reagent to quantitate relative changes in protein levels of the hippocampal PSD in response to morphine administration.

Regulation (changes) of PSD associated with morphine

1) Confidence 0.35 Published 2007 Journal Mol. Cell Proteomics Section Abstract Doc Link 17028301 Disease Relevance 0 Pain Relevance 0.31

After stimulation, however, we find no change in the size of the bouton–spine interface, size of the PSD, or the total contribution made by the astrocyte to the neuropil that we sample, but a significant increase in astrocytic envelopment that remains proportional to synaptic size.

Neg (no) Regulation (change) of PSD in astrocyte

2) Confidence 0.32 Published 2006 Journal PLoS Biology Section Body Doc Link PMC1609127 Disease Relevance 0.05 Pain Relevance 0.20

Because no changes were observed in total hippocampus homogenate, the specific decrease in the PSD is therefore attributed to changes in glutamate receptor subunit trafficking or clustering in the PSD instead of a reduced protein translation.

Regulation (changes) of PSD in hippocampus associated with glutamate receptor and hippocampus

3) Confidence 0.18 Published 2007 Journal Environ Health Perspect Section Body Doc Link PMC1892123 Disease Relevance 0.23 Pain Relevance 0.37

Specifically, PSD93 is known to play a role in stabilization of receptor clusters at neuronal cholinergic synapses, and PSD scaffolding molecules are linked to cytoskeletal processes, transcriptional regulation and protein phosphorylation, and can relay the intracellular effects of synaptic activation [83].

Regulation (regulation) of PSD in synapses

4) Confidence 0.15 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2816210 Disease Relevance 0 Pain Relevance 0.10

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