INT140003

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Context Info
Confidence 0.74
First Reported 2007
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 4
Total Number 21
Disease Relevance 10.75
Pain Relevance 0.87

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
neurites 11
neurons 2
neuronal 1
Wld (Mus musculus)
Pain Link Frequency Relevance Heat
induced neuropathy 39 99.92 Very High Very High Very High
vincristine 6 99.54 Very High Very High Very High
dorsal root ganglion 54 84.60 Quite High
imagery 90 70.56 Quite High
Immobilon 18 63.24 Quite High
Taxol 36 21.36 Low Low
Multiple sclerosis 18 11.04 Low Low
Sciatic nerve 18 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Wallerian Degeneration 1569 100.00 Very High Very High Very High
Neuropathic Pain 39 99.92 Very High Very High Very High
Targeted Disruption 270 99.88 Very High Very High Very High
Neurodegenerative Disease 21 94.76 High High
Death 162 92.92 High High
Stress 54 90.24 High High
Injury 309 89.52 High High
Ganglion Cysts 54 84.60 Quite High
Toxicity 18 76.68 Quite High
Apoptosis 18 66.64 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Importantly, WldS expression not only delays the onset of neurite degeneration following protein synthesis suppression, it also delays their commitment to degenerate at least 3-fold (Figure S7B).
Gene_expression (expression) of WldS in neurite associated with wallerian degeneration
1) Confidence 0.74 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.30 Pain Relevance 0
Products were cloned into pCMV Tag-2B (Stratagene) to generate FLAG-Nmnat/WldS expression vectors or pEGFP-N1 (BD Biosciences Clontech) to generate a Nmnat2-eGFP expression vector.
Gene_expression (expression) of WldS associated with wallerian degeneration
2) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.36 Pain Relevance 0
The absence of significant axon protection in transgenic mice expressing Nmnat1 in neurons at similar levels to WldS in WldS neurons [4],[8] suggests either that Nmnat1 localization is more rigorously controlled in vivo or that Nmnat1 overexpression is greater in vitro.
Gene_expression (expressing) of WldS in neurons associated with targeted disruption and wallerian degeneration
3) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.77 Pain Relevance 0
In Figures 6C, 7A, 7B, and 7C, exactly 10% of the extract that was used for the blot probed with the Nmnat2 antibody (and WldS and Histones antibodies where applicable) was used for the blot probed with the ?
Gene_expression (where) of WldS associated with wallerian degeneration
4) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.18 Pain Relevance 0.12
It is also consistent with WldS-mediated protection of neurites (Figure 4D) being able to reduce siNmnat2-associated neuronal loss to control levels (Figure S6C), despite the fact that WldS cannot directly prevent neuronal cell death in SCG cultures [35].
Gene_expression (protection) of WldS-mediated in neuronal associated with wallerian degeneration and death
5) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.95 Pain Relevance 0
The continued presence of WldS in transected WldS neurites long after Nmnat2 is lost shows that WldS does not act by stabilizing Nmnat2 but instead supports a model in which WldS substitutes for the functionally related Nmnat2.


Gene_expression (presence) of WldS in neurites associated with wallerian degeneration
6) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.89 Pain Relevance 0
For turnover experiments (Figure 6), cells in a 12-well dish format were co-transfected with 100 ng each of the FLAG-Nmnat1, FLAG-Nmnat-3, and FLAG-WldS expression constructs and 250 ng of the FLAG-Nmnat-2 expression construct.
Gene_expression (expression) of WldS associated with wallerian degeneration
7) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.10 Pain Relevance 0.03
Similarly, localized translation is not required in injured neurites for WldS-mediated protection, and it is also not needed for Wallerian degeneration itself (Figure S2).
Gene_expression (protection) of WldS-mediated in neurites associated with wallerian degeneration
8) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.95 Pain Relevance 0.08
Nmnat2 levels in WldS neurites fell with a remarkably similar time course to those in wild-type neurites.
Gene_expression (levels) of WldS in neurites associated with wallerian degeneration
9) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.54 Pain Relevance 0
As predicted, WldS levels in neurites also remained relatively constant.
Gene_expression (levels) of WldS in neurites associated with wallerian degeneration
10) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.80 Pain Relevance 0
Second, the inability of Nmnat2 to protect 5-d lesioned axons in Drosophila, unlike the other Nmnat isoforms and WldS, initially suggested it was either ineffective or by far the least potent isoform [2].
Gene_expression (isoforms) of WldS associated with wallerian degeneration
11) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.23 Pain Relevance 0
In Figures 6C, 7A, 7B, and 7C, exactly 10% of the extract that was used for the blot probed with the Nmnat2 antibody (and WldS and Histones antibodies where applicable) was used for the blot probed with the ?
Gene_expression (applicable) of WldS associated with wallerian degeneration
12) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.18 Pain Relevance 0.12
Importantly, continued degradation of Nmnat2 in WldS neurites rules out an alternative hypothesis, that WldS could delay axon degeneration by stabilizing Nmnat2.
Gene_expression (neurites) of WldS in neurites associated with wallerian degeneration
13) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.79 Pain Relevance 0
Products were cloned into pCMV Tag-2B (Stratagene) to generate FLAG-Nmnat/WldS expression vectors or pEGFP-N1 (BD Biosciences Clontech) to generate a Nmnat2-eGFP expression vector.
Gene_expression (expression) of WldS associated with wallerian degeneration
14) Confidence 0.64 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.36 Pain Relevance 0
Protection of vincristine-induced neuropathy by WldS expression and the independence of the activity of Nmnat1.
Gene_expression (expression) of WldS associated with vincristine, induced neuropathy and neuropathic pain
15) Confidence 0.63 Published 2007 Journal Neurosci. Lett. Section Title Doc Link 17113230 Disease Relevance 0.35 Pain Relevance 0.19
We generated WldS or Nmnat1-overexpressing Neuro2A cell lines, in which neuronal differentiation including neurite elongation can be induced by retinoic acid.
Gene_expression (overexpressing) of WldS in neurite
16) Confidence 0.63 Published 2007 Journal Neurosci. Lett. Section Abstract Doc Link 17113230 Disease Relevance 0.33 Pain Relevance 0.16
The overexpression of WldS delayed the neurite degeneration by vincristine, whereas that of Nmnat1 did not delay it much.
Gene_expression (overexpression) of WldS in neurite associated with vincristine
17) Confidence 0.63 Published 2007 Journal Neurosci. Lett. Section Abstract Doc Link 17113230 Disease Relevance 0.33 Pain Relevance 0.16
Indeed, levels of WldS protein are only moderately reduced in neurites 48 h after transection (Figure S8).
Gene_expression (levels) of WldS in neurites associated with wallerian degeneration
18) Confidence 0.58 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.82 Pain Relevance 0
We tested the ability of exogenous expression of tagged Nmnat2 and WldS to protect transected neurites in a microinjection-based assay (Figure S9).
Gene_expression (expression) of WldS in neurites associated with wallerian degeneration
19) Confidence 0.58 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.34 Pain Relevance 0
To confirm that the siNmnat2-induced neurite degeneration is Wallerian-like, we microinjected WldS neurons with siNmnat2 and found degeneration was completely blocked for at least 72 h (Figure 4A and 4D).
Gene_expression (neurons) of WldS in neurons associated with wallerian degeneration
20) Confidence 0.56 Published 2010 Journal PLoS Biology Section Body Doc Link PMC2811159 Disease Relevance 0.28 Pain Relevance 0

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