INT140794

Context Info Confidence 0.64 First Reported 2005 Last Reported 2010 Negated 0 Speculated 1 Reported most in Body Documents 22 Total Number 23 Disease Relevance 9.41 Pain Relevance 0.83

transport (Smad3) intracellular (Smad3) DNA binding (Smad3) protein complex (Smad3) cytoplasm (Smad3) nucleus (Smad3)

Anatomy Link Frequency chondrocytes 3 plaques 2 articular 1 hematopoietic stem cell 1 retinas 1 Smad3 (Mus musculus)

Pain Link Frequency Relevance Heat Pain 8 96.16 Inflammation 140 90.96 Osteoarthritis 546 85.44 metalloproteinase 37 80.48 fibrosis 37 78.96 cytokine 64 61.60 ischemia 2 46.92 dexamethasone 2 44.40 Kinase C 2 25.64 Central nervous system 22 5.00

Disease Link Frequency Relevance Heat Disease 95 99.92 Osteoporosis 5 99.84 Pulmonary Disease 144 99.74 Osteosclerosis 12 99.08 Cataract 204 99.04 Repression 49 98.16 Infection 10 97.20 Hypertrophy 30 96.92 Fibrosis 37 96.20 Pain 2 96.16

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Immunohistochemistry (Smad3 And p-Smad2/3)

Gene_expression (/) of Smad3

1) Confidence 0.64 Published 2005 Journal BMC Cancer Section Body Doc Link PMC1177930 Disease Relevance 0 Pain Relevance 0

Expression of Smad3 and Sox17 inputs was assessed by immunoblot using cell lysates from transfections done in parallel to chromatin immunoprecipitations.

Gene_expression (Expression) of Smad3

2) Confidence 0.56 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0

Overexpression of both Smad2 and Smad3 blocked spontaneous maturation in Smad3-deficient chondrocytes [70,71].

Gene_expression (Overexpression) of Smad3 in chondrocytes

3) Confidence 0.54 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875624 Disease Relevance 0.19 Pain Relevance 0.03

on chondrocyte maturation is mediated by the Smad2/3 pathway, as has been shown by overexpression of dominant negative Smad2 and Smad3 in chondrocytes.

Gene_expression (overexpression) of Smad3 in chondrocyte

4) Confidence 0.54 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875624 Disease Relevance 0.20 Pain Relevance 0

In articular chondrocytes treated with azacytidine, reduced Smad2 and Smad3 expression and signaling and increased Smad1/5 expression correlated with elevated synthesis of type X collagen and alkaline phosphatase.

Gene_expression (expression) of Smad3 in articular

5) Confidence 0.54 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875624 Disease Relevance 0.44 Pain Relevance 0.03

SMA expression is not Smad3-dependent in that Smad3 deficient mice can develop small ASC plaques that are ?

Gene_expression (expression) of Smad3 in plaques associated with cataract

6) Confidence 0.50 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2647562 Disease Relevance 0.98 Pain Relevance 0.04

1 model to the Smad3 deficient mice confirmed our earlier findings demonstrating that while Smad3 is sufficient for, it is not necessary for the EMT of LECs during ASC formation in vivo.

Gene_expression (deficient) of Smad3 associated with cataract

7) Confidence 0.50 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2647562 Disease Relevance 0.65 Pain Relevance 0

SMA expression is not Smad3-dependent in that Smad3 deficient mice can develop small ASC plaques that are ?

Gene_expression (deficient) of Smad3 in plaques associated with cataract

8) Confidence 0.50 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2647562 Disease Relevance 0.92 Pain Relevance 0.04

Levels of prolyl 4-hydroxylase beta (P4Hbeta), cytoglobin/stellate cell activation-associated protein (Cygb/STAP), transgelin, tissue inhibitors of metalloproteinases, serum response factor, transforming growth factor beta (TGFbeta), Smad3, and pancreatitis-associated protein 1 (PAP-1) were determined by immunohistochemistry.

Spec (determined) Gene_expression (Levels) of Smad3 in stellate cell

9) Confidence 0.49 Published 2009 Journal Pancreas Section Body Doc Link 19188864 Disease Relevance 0.10 Pain Relevance 0

Rik Derynck (UCSF) [64], and the FLAG-tagged Smad3 expression vectors were generated by Dr.

Gene_expression (expression) of Smad3

10) Confidence 0.49 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0.09 Pain Relevance 0

Sox17-V5 and FLAG-Smad3 constructs were expressed in MLE-15 cells.

Gene_expression (expressed) of Smad3

11) Confidence 0.49 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0

The GST-Sox17 and GST-Smad3 fusion proteins (and GST only) were expressed in BL21 cells induced with 1 mM isopropyl ?

Gene_expression (expressed) of Smad3

12) Confidence 0.49 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0.06 Pain Relevance 0

1 and Smad3, consistent with an antagonistic effect of Sox17 on the TGF-?

Gene_expression (consistent) of Smad3

13) Confidence 0.49 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0.32 Pain Relevance 0

/Smad3 Signaling to Initiate Progenitor Cell Behavior in the Respiratory Epithelium The Sry-related high mobility group box transcription factor Sox17 is required for diverse developmental processes including endoderm formation, vascular development, and fetal hematopoietic stem cell maintenance.

Gene_expression (required) of Smad3 in hematopoietic stem cell

14) Confidence 0.49 Published 2009 Journal PLoS ONE Section Title Doc Link PMC2682659 Disease Relevance 0 Pain Relevance 0

Phosphorylated Smad2 and Smad3, the effectors of the TGF-?

Gene_expression (effectors) of Smad3

15) Confidence 0.49 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2682659 Disease Relevance 0.09 Pain Relevance 0

Overexpression of dominant negative Smad3 causes neurodegeneration in cell cultures, indicating that loss of Smad2/3 signaling is involved.

Gene_expression (Overexpression) of Smad3

16) Confidence 0.47 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875624 Disease Relevance 1.06 Pain Relevance 0.17

Interaction of Runx2 with Smad1 facilitates the function of Runx2 in stimulating terminal differentiation, while Smad3 blocks Runx2 function [81-83].

Gene_expression (function) of Smad3

17) Confidence 0.47 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875624 Disease Relevance 0.59 Pain Relevance 0.16

The ALK1 (Smad1/5/8) and ALK5 (Smad3) signaling balance in chondrocytes apparently determines MMP-13 expression.

Gene_expression (/) of Smad3 in chondrocytes

18) Confidence 0.47 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875624 Disease Relevance 0.26 Pain Relevance 0.06

A previous study demonstrated that Smad3, signal transducers of the TGF-?

Gene_expression (signal) of Smad3

19) Confidence 0.45 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2944871 Disease Relevance 0.08 Pain Relevance 0

Genes monitored were smad2, smad3, TGF-?

Gene_expression (Genes) of smad3

20) Confidence 0.39 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2659748 Disease Relevance 0.27 Pain Relevance 0

General Comments

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