INT141180

Context Info Confidence 0.74 First Reported 2006 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 6 Total Number 6 Disease Relevance 5.77 Pain Relevance 2.98

cytosol (Apaf1) Golgi apparatus (Apaf1) nucleus (Apaf1) intracellular (Apaf1) cytoplasm (Apaf1)

Anatomy Link Frequency dorsal horn spinal cord 2 Apaf1 (Mus musculus)

Pain Link Frequency Relevance Heat Eae 18 99.50 Dorsal horn 6 99.04 allodynia 3 99.00 Neuropathic pain 12 98.60 Spinal cord 15 98.56 Thermal hyperalgesia 3 98.50 Sciatic nerve 9 96.32 Inflammation 12 50.00 Pain 9 25.00 cytokine 5 5.00

Disease Link Frequency Relevance Heat Apoptosis 101 100.00 Coronary Artery Disease 3 99.80 Injury 20 99.50 Neuropathic Pain 15 99.00 Hyperalgesia 3 98.50 Frailty 17 93.76 Cancer 119 82.12 Neurodegenerative Disease 7 59.12 Hyperplasia 1 53.12 INFLAMMATION 13 50.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

RT-PCR analysis showed increased expression of bax, apoptotic protease-activating factor-1 (apaf-1), and caspase-9 in the dorsal horn spinal cord 3 days after chronic constriction injury of sciatic nerve.

Gene_expression (expression) of apoptotic protease-activating factor-1 in dorsal horn spinal cord associated with eae, injury, dorsal horn, sciatic nerve, apoptosis and spinal cord

1) Confidence 0.74 Published 2007 Journal Pharmacol. Res. Section Abstract Doc Link 17207636 Disease Relevance 1.16 Pain Relevance 0.92

RT-PCR analysis showed increased expression of bax, apoptotic protease-activating factor-1 (apaf-1), and caspase-9 in the dorsal horn spinal cord 3 days after chronic constriction injury of sciatic nerve.

Gene_expression (expression) of apaf-1 in dorsal horn spinal cord associated with eae, injury, dorsal horn, sciatic nerve, apoptosis and spinal cord

2) Confidence 0.74 Published 2007 Journal Pharmacol. Res. Section Abstract Doc Link 17207636 Disease Relevance 1.17 Pain Relevance 0.93

of the proapoptotic genes Fas, FasL, p75NGFR, p53, Bax, caspase-3, and CAD, whereas expression of Apaf-1 and the anti-apoptotic gene Bcl-2 was unchanged (Figure 3D).

Gene_expression (expression) of Apaf-1 associated with coronary artery disease and apoptosis

3) Confidence 0.60 Published 2006 Journal Environ Health Perspect Section Body Doc Link PMC1513335 Disease Relevance 1.15 Pain Relevance 0

As discussed, many of the most crucial members of apoptotic pathways for these organ sites can be targeted MMR-deficient mutation, most notably BAX, APAF-1, BCL10, CASP-5, FAS and others (105).

Gene_expression (mutation) of APAF-1 associated with apoptosis

4) Confidence 0.36 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1361617 Disease Relevance 0.76 Pain Relevance 0

More specifically, there was no significant reduction in the expression levels of Chk1, Brca1, Topbp1, Rad51, Ddb2, p73, Apaf1, or caspases which were all described to show reduced expression following MCPH1 knockdowns [34] (Tables S3, S4, S5).

Gene_expression (expression) of Apaf1

5) Confidence 0.33 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2821930 Disease Relevance 0.12 Pain Relevance 0

Administration of phenyl-N-tert-butylnitrone (PBN), a potent ROS scavenger, reduced the development of thermal hyperalgesia and mechanical allodynia at 1 and 3 days post-CCI, and decreased the mRNA levels of bax, apaf-1, and caspase-9.

Gene_expression (levels) of apaf-1 associated with allodynia, eae and thermal hyperalgesia

6) Confidence 0.25 Published 2007 Journal Pharmacol. Res. Section Abstract Doc Link 17207636 Disease Relevance 1.41 Pain Relevance 1.14

General Comments

This test has worked.