INT145532

From wiki-pain
Jump to: navigation, search
Context Info
Confidence 0.79
First Reported 2005
Last Reported 2010
Negated 3
Speculated 1
Reported most in Body
Documents 25
Total Number 26
Disease Relevance 1.97
Pain Relevance 4.36

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

structural molecule activity (Dlg4) plasma membrane (Dlg4) protein complex assembly (Dlg4)
cytoplasm (Dlg4)
Anatomy Link Frequency
neuronal 3
neurons 3
spines 3
forebrain 2
synapses 2
Dlg4 (Mus musculus)
Pain Link Frequency Relevance Heat
Substantia nigra 18 99.08 Very High Very High Very High
addiction 28 98.72 Very High Very High Very High
Morphine 20 98.60 Very High Very High Very High
Neurotransmitter 57 97.60 Very High Very High Very High
Glutamate 43 95.64 Very High Very High Very High
Hippocampus 86 95.28 Very High Very High Very High
cocaine 36 94.96 High High
Spinal cord 33 94.72 High High
Nicotine 10 94.48 High High
nMDA receptor 166 94.16 High High
Disease Link Frequency Relevance Heat
Drug Dependence 16 98.72 Very High Very High Very High
Nociception 6 97.96 Very High Very High Very High
Cognitive Disorder 47 96.76 Very High Very High Very High
Targeted Disruption 146 91.76 High High
Congenital Anomalies 41 88.24 High High
Pain 24 83.92 Quite High
Adhesions 18 83.16 Quite High
Bordatella Infection 1 70.88 Quite High
Anxiety Disorder 34 70.48 Quite High
Parkinson's Disease 15 69.92 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The fourth and final stage was the release of the PSD-95 complex from the column using imidazole, completing the tandem purification.
Localization (release) of PSD-95
1) Confidence 0.79 Published 2009 Journal Mol Syst Biol Section Body Doc Link PMC2694677 Disease Relevance 0.06 Pain Relevance 0
In the absence of TEV, there was no spontaneous release of TAP-tagged PSD-95 during incubations (Figure 3B, lane 4, 5 Non-TEV, El lane).
Localization (release) of TAP-tagged PSD-95
2) Confidence 0.79 Published 2009 Journal Mol Syst Biol Section Body Doc Link PMC2694677 Disease Relevance 0 Pain Relevance 0
PSD-95 is localized to the postsynaptic compartment in which it interacts with neurotransmitter receptors and ion channels to assemble signaling complexes (Kornau et al, 1995; Hunt et al, 1996; Tu et al, 1999; Husi et al, 2000; Nehring et al, 2000; Dosemeci et al, 2007) controlling neuronal plasticity (Migaud et al, 1998; Carlisle et al, 2008; Cuthbert et al, 2007) underlying learning and memory (Migaud et al, 1998), pain (Garry et al, 2003) and drug addiction (Yao et al, 2004).
Localization (localized) of PSD-95 in neuronal associated with drug dependence, addiction, pain and neurotransmitter
3) Confidence 0.73 Published 2009 Journal Mol Syst Biol Section Body Doc Link PMC2694677 Disease Relevance 0.24 Pain Relevance 0.17
The specific subcellular localization of PSD-95 to the postsynaptic compartment of synapses (dendritic spines) was monitored using postsynaptic markers for glutamate neurotransmitter receptors (GluR1 or NR1), presynaptic marker (synaptophysin) and dendritic markers (MAP2) (Figure 2B and Supplementary Figure 1).
Localization (localization) of PSD-95 in synapses associated with neurotransmitter and glutamate
4) Confidence 0.73 Published 2009 Journal Mol Syst Biol Section Body Doc Link PMC2694677 Disease Relevance 0 Pain Relevance 0.14
The forebrain tissue was solubilized from heterozygous (PSD-95TAP/+) mice and PSD-95 was immunoprecipitated and immunoblotted using an anti-PSD-95 antibody (Figure 1D).
Localization (immunoprecipitated) of PSD-95 in forebrain
5) Confidence 0.73 Published 2009 Journal Mol Syst Biol Section Body Doc Link PMC2694677 Disease Relevance 0 Pain Relevance 0
-end homolog PSD-95 sequence of 6384 bp and a 3?
Localization (sequence) of PSD-95
6) Confidence 0.73 Published 2009 Journal Mol Syst Biol Section Body Doc Link PMC2694677 Disease Relevance 0 Pain Relevance 0
Our data show that PSD-95 is retained by individual PSDs for 1 h or less.
Localization (retained) of PSD-95
7) Confidence 0.73 Published 2006 Journal PLoS Biology Section Body Doc Link PMC1634879 Disease Relevance 0 Pain Relevance 0
For other controls, as expected, the FLAG antibody did not precipitate PSD-95 from wt mouse forebrain (Figure 3B, right panel).
Neg (not) Localization (precipitate) of PSD-95 in forebrain
8) Confidence 0.69 Published 2009 Journal Mol Syst Biol Section Body Doc Link PMC2694677 Disease Relevance 0 Pain Relevance 0
Optimization of single-step and tandem affinity purification of PSD-95-associated complexes
Localization (Optimization) of PSD-95
9) Confidence 0.64 Published 2009 Journal Mol Syst Biol Section Body Doc Link PMC2694677 Disease Relevance 0.08 Pain Relevance 0.06
To examine the synaptic localization of TAP-tagged PSD-95, we cultured embryonic hippocampal neurons from PSD-95TAP/TAP and wt mice.
Spec (examine) Localization (localization) of TAP-tagged PSD-95 in neurons
10) Confidence 0.64 Published 2009 Journal Mol Syst Biol Section Body Doc Link PMC2694677 Disease Relevance 0 Pain Relevance 0.12
TEV incubation released 50–70% of cleaved PSD-95 (Figure 3B, lane 2, El) and 30–50% of cleaved PSD-95 remained on the beads (Figure 3B, lane 3, BB).
Localization (released) of PSD-95
11) Confidence 0.60 Published 2009 Journal Mol Syst Biol Section Body Doc Link PMC2694677 Disease Relevance 0 Pain Relevance 0
We found that the immunoprecipitation of PSD-95 was reduced when Ca2+ is increased (Figure 6C).
Localization (immunoprecipitation) of PSD-95
12) Confidence 0.56 Published 2010 Journal Mol Brain Section Body Doc Link PMC2822766 Disease Relevance 0 Pain Relevance 0.32
As a control, labeling of PSD95 was always observed at synaptic locations (Figure 2E, F).
Localization (labeling) of PSD95
13) Confidence 0.38 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3013137 Disease Relevance 0 Pain Relevance 0.69
Negative control antibodies did not immunoprecipitate either Neto1 or PSD-95 (Figures 2E and 3A).
Neg (not) Localization (immunoprecipitate) of PSD-95
14) Confidence 0.37 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0.06
20HA co-immunoprecipitated with both NR2A (Figure 5A, lanes 1 and 2, and Figure 5C, lane 4) and NR2B (Figure 5B, lanes 1 and 2, and Figure 5C, lane 5) expressed in the absence of NR1 and PSD-95.
Neg (absence) Localization (absence) of PSD-95
15) Confidence 0.37 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0 Pain Relevance 0
The absence of Neto1 had no effect on the overall abundance of NR1, NR2A, NR2B, PSD-95, GluR2, VAMP2, or GABAAR1 proteins (Figure 6L) in whole brain extracts, or of NR1, NR2A, NR2B, or PSD-95 in crude synaptosomes (Figure 6M).
Localization (abundance) of PSD-95 in brain
16) Confidence 0.37 Published 2009 Journal PLoS Biology Section Body Doc Link PMC2652390 Disease Relevance 0.17 Pain Relevance 0.05
Using an antibody for the protein PSD-95, which is found at post-synaptic sites and within dendritic spines, we observed extensive colocalization of PSD-95 with hCAR in both the SN and STR (Figure 7A–H).
Localization (colocalization) of PSD-95 in spines associated with substantia nigra
17) Confidence 0.34 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2941453 Disease Relevance 0.15 Pain Relevance 0.23
PSD-95 and its relation to pan-AMPAr staining
Localization (relation) of PSD-95
18) Confidence 0.24 Published 2008 Journal Mol Pain Section Body Doc Link PMC2248168 Disease Relevance 0.09 Pain Relevance 0.17
Immunocytochemical analysis of the hippocampal neurons in culture revealed that EP2 highly colocalized with synaptophysin (71.0%), but partially with PSD-95 (14.2%), whereas EP3 partially colocalized with PSD-95 (45.3%) or synaptophysin (38.7%) (Zhu et al. 2005).
Localization (colocalized) of PSD-95 in neurons
19) Confidence 0.18 Published 2007 Journal Gene Regulation and Systems Biology Section Body Doc Link PMC2759147 Disease Relevance 0 Pain Relevance 0.11
In cultures of neurons from the visual cortex, however, both EP2 and EP3 are highly colocalized with PSD-95 (EP2, 71%; EP3, 82%), but less colocalized with synaptophysin (EP2, 25%; EP3, 13%) (Akaneya and Tsumoto, 2006).
Localization (colocalized) of PSD-95 in neurons
20) Confidence 0.18 Published 2007 Journal Gene Regulation and Systems Biology Section Body Doc Link PMC2759147 Disease Relevance 0 Pain Relevance 0.14

General Comments

This test has worked.

Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox