INT146191

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Context Info
Confidence 0.67
First Reported 2005
Last Reported 2010
Negated 1
Speculated 7
Reported most in Body
Documents 32
Total Number 39
Disease Relevance 17.63
Pain Relevance 2.92

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell differentiation (Rhoa) mitochondrion (Rhoa) cell morphogenesis (Rhoa)
intracellular (Rhoa) GTPase activity (Rhoa) cytoplasm (Rhoa)
Anatomy Link Frequency
bar 2
plasma 1
BSM 1
spinal cord 1
neuronal 1
Rhoa (Mus musculus)
Pain Link Frequency Relevance Heat
Inflammation 131 100.00 Very High Very High Very High
Spinal cord 27 99.82 Very High Very High Very High
intrathecal 9 99.40 Very High Very High Very High
Demyelination 5 97.64 Very High Very High Very High
Central nervous system 29 93.76 High High
Dorsal horn 4 92.56 High High
substance P 26 91.12 High High
Inflammatory response 1 90.68 High High
cytokine 27 89.52 High High
Neuropathic pain 8 89.44 High High
Disease Link Frequency Relevance Heat
INFLAMMATION 141 100.00 Very High Very High Very High
Spinal Cord Injury 16 99.96 Very High Very High Very High
Apoptosis 308 99.92 Very High Very High Very High
Mevalonate Kinase Deficiency 928 99.84 Very High Very High Very High
Death 94 99.44 Very High Very High Very High
Nervous System Injury 18 99.44 Very High Very High Very High
Demyelinating Disease 5 97.64 Very High Very High Very High
Clostridium Infection 24 94.04 High High
Asthma 72 92.44 High High
Atherosclerosis 210 91.92 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The expression of RhoA protein in BSM homogenates was assessed by using immunoblotting.
Gene_expression (expression) of RhoA protein
1) Confidence 0.67 Published 2005 Journal Respir Res Section Body Doc Link PMC545934 Disease Relevance 0 Pain Relevance 0
Furthermore, western blot analysis clearly demonstrated a distinct expression of RhoA protein in BSMs of mice (Fig. 4).
Gene_expression (expression) of RhoA protein
2) Confidence 0.67 Published 2005 Journal Respir Res Section Body Doc Link PMC545934 Disease Relevance 0 Pain Relevance 0.07
Thus, the current study demonstrated an augmentation of ACh-induced, RhoA-mediated Ca2+ sensitization of BSM contraction, which coincides with enhanced protein expression of RhoA, in antigen-induced AHR.
Gene_expression (expression) of RhoA
3) Confidence 0.67 Published 2005 Journal Respir Res Section Body Doc Link PMC545934 Disease Relevance 0.35 Pain Relevance 0.09
Intrathecal injection of mevalonate also produced RhoA translocation from cytosol to plasma membrane.
Gene_expression (produced) of RhoA in plasma associated with intrathecal
4) Confidence 0.59 Published 2008 Journal Pain Section Abstract Doc Link 17764839 Disease Relevance 0.71 Pain Relevance 0.76
Determination of RhoA protein level in BSM
Gene_expression (level) of RhoA protein
5) Confidence 0.58 Published 2005 Journal Respir Res Section Body Doc Link PMC545934 Disease Relevance 0.16 Pain Relevance 0
To determine the level of RhoA protein in BSMs, the samples (10 ?
Spec (determine) Gene_expression (level) of RhoA protein
6) Confidence 0.58 Published 2005 Journal Respir Res Section Body Doc Link PMC545934 Disease Relevance 0 Pain Relevance 0
A participation of RhoA-mediated Ca2+ sensitization in the augmented ACh-induced contraction of BSM was demonstrated in this animal model of AHR.


Gene_expression (participation) of RhoA in BSM
7) Confidence 0.58 Published 2005 Journal Respir Res Section Body Doc Link PMC545934 Disease Relevance 0.39 Pain Relevance 0.09
As shown in Fig. 4A, immunoblotting with the antibody against RhoA gave a single 21 kD band, indicating the expression of RhoA protein in murine BSM.
Gene_expression (expression) of RhoA protein in band
8) Confidence 0.58 Published 2005 Journal Respir Res Section Body Doc Link PMC545934 Disease Relevance 0 Pain Relevance 0
These observations are in a good contrast with the previous findings that the nerve injury-induced activation of LPA1 receptor and RhoA causes functional changes in myelinated A-fibers, such as demyelination and up-regulation of Ca2+ channel ?
Gene_expression (receptor) of RhoA in nerve associated with demyelination and nervous system injury
9) Confidence 0.53 Published 2006 Journal Mol Pain Section Body Doc Link PMC1562366 Disease Relevance 1.16 Pain Relevance 0.97
Moreover, protein expression of RhoA was significantly increased in the hyperresponsive BSMs.


Gene_expression (expression) of RhoA
10) Confidence 0.52 Published 2005 Journal Respir Res Section Abstract Doc Link PMC545934 Disease Relevance 0.16 Pain Relevance 0
The contractility and RhoA protein expression of BSMs were measured by organ-bath technique and immunoblotting, respectively.


Gene_expression (expression) of RhoA protein
11) Confidence 0.52 Published 2005 Journal Respir Res Section Abstract Doc Link PMC545934 Disease Relevance 0.17 Pain Relevance 0
The levels of RhoA protein in soluble fractions increased in both control and MKD cells (Fig. 1c).
Gene_expression (levels) of RhoA protein associated with mevalonate kinase deficiency
12) Confidence 0.49 Published 2010 Journal J Inherit Metab Dis Section Body Doc Link PMC2946549 Disease Relevance 0.93 Pain Relevance 0
Again, MKD cells were more sensitive to treatment with simvastatin, with markedly increased levels of active soluble RhoA and decreased levels of active membrane-bound RhoA already observed after incubation with low concentrations of simvastatin.
Gene_expression (levels) of RhoA associated with mevalonate kinase deficiency
13) Confidence 0.49 Published 2010 Journal J Inherit Metab Dis Section Body Doc Link PMC2946549 Disease Relevance 0.63 Pain Relevance 0
In separate experiments, equal amounts of cell lysate (d, i) were analyzed for relative levels of active RhoA (e) and Rac1 (j).
Spec (analyzed) Gene_expression (levels) of RhoA
14) Confidence 0.49 Published 2010 Journal J Inherit Metab Dis Section Body Doc Link PMC2946549 Disease Relevance 0.29 Pain Relevance 0
Equal amounts of cell lysate (a, f) were analyzed for relative levels of RhoA (b) and Rac1 (g) associated with membranes and relative soluble levels of RhoA (c) and Rac1 (h).
Spec (analyzed) Gene_expression (levels) of RhoA
15) Confidence 0.49 Published 2010 Journal J Inherit Metab Dis Section Body Doc Link PMC2946549 Disease Relevance 0.28 Pain Relevance 0
Equal amounts of cell lysate (a, d) were analyzed for relative levels of active RhoA (b) and active Rac1 (e) associated with the membranes (white bar set as 100%) and relative soluble levels of active RhoA (c) and active Rac1 (f) (black bar set as 100%).
Spec (analyzed) Gene_expression (levels) of RhoA in bar
16) Confidence 0.49 Published 2010 Journal J Inherit Metab Dis Section Body Doc Link PMC2946549 Disease Relevance 0.44 Pain Relevance 0
In separate experiments, equal amounts of cell lysate (d, i) were analyzed for relative levels of active RhoA (e) and Rac1 (j).
Spec (analyzed) Gene_expression (levels) of RhoA
17) Confidence 0.49 Published 2010 Journal J Inherit Metab Dis Section Body Doc Link PMC2946549 Disease Relevance 0.53 Pain Relevance 0
When control and MKD cells were cultured in the absence of simvastatin followed by separation into membrane and soluble fractions, we observed somewhat higher levels of membrane-bound RhoA in MKD cells than in control cells (Fig. 1b, immunoblots), whereas membrane-bound Rac1 levels were slightly lower in MKD cells than in control cells (Fig.1g, immunoblots).
Gene_expression (bound) of RhoA associated with mevalonate kinase deficiency
18) Confidence 0.49 Published 2010 Journal J Inherit Metab Dis Section Body Doc Link PMC2946549 Disease Relevance 0.74 Pain Relevance 0
Activated RhoA and Rac1 assessment assays
Gene_expression (assays) of RhoA
19) Confidence 0.49 Published 2010 Journal J Inherit Metab Dis Section Body Doc Link PMC2946549 Disease Relevance 0 Pain Relevance 0
Combination of membrane and soluble fraction separation and activated RhoA and Rac1 assessment assays
Gene_expression (assays) of RhoA
20) Confidence 0.49 Published 2010 Journal J Inherit Metab Dis Section Body Doc Link PMC2946549 Disease Relevance 0 Pain Relevance 0

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