INT146511

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Context Info
Confidence 0.78
First Reported 2007
Last Reported 2010
Negated 2
Speculated 1
Reported most in Body
Documents 25
Total Number 42
Disease Relevance 8.67
Pain Relevance 1.55

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell differentiation (Nrp2) cell adhesion (Nrp2) plasma membrane (Nrp2)
Anatomy Link Frequency
neuronal 3
F11 2
endothelial cells 2
neurons 2
corneas 2
Nrp2 (Mus musculus)
Pain Link Frequency Relevance Heat
adenocard 1 100.00 Very High Very High Very High
endometriosis 9 93.76 High High
Spinal cord 138 87.08 High High
Versed 93 86.40 High High
Inflammation 102 85.16 High High
pain pelvic 1 70.64 Quite High
anesthesia 47 70.52 Quite High
GABAergic 5 63.80 Quite High
Pyramidal cell 17 62.24 Quite High
imagery 39 34.08 Quite Low
Disease Link Frequency Relevance Heat
Targeted Disruption 166 99.80 Very High Very High Very High
Injury 120 98.80 Very High Very High Very High
Neuroblastoma 23 96.68 Very High Very High Very High
Cancer 90 96.60 Very High Very High Very High
Toxicity 253 96.40 Very High Very High Very High
Lewis Lung Carcinoma 1 96.36 Very High Very High Very High
Endometriosis (extended) 9 93.76 High High
Experimental Melanoma 1 91.28 High High
Overdose 46 88.68 High High
Cytomegalovirus Infection 92 88.52 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
These data demonstrate that decreasing NP2 expression has no direct effect on corneal macrophages trafficking to regional lymph nodes, and further confirm the selective effect of NP2 knockdown in the inflamed cornea.
Gene_expression (expression) of NP2 in lymph nodes
1) Confidence 0.78 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0.33 Pain Relevance 0.03
To further evaluate whether NP2-amiR could inhibit NP2 expression in vivo, corneas were subjected to intrastromal delivery of NP2-amiR and harvested on day 7 after suture placement.
Gene_expression (expression) of NP2 in corneas
2) Confidence 0.78 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0 Pain Relevance 0
Furthermore, NP2 is not expressed in corneal macrophage (data not shown), and NP2 knockdown had no apparent effect on the macrophages recruitment into vascularized beds at the time of corneal transplantation.
Neg (not) Gene_expression (expressed) of NP2 in macrophages
3) Confidence 0.78 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0.38 Pain Relevance 0.04
To determine the effect of reduced NP2 expression on cell infiltration associated with corneal suture injury in recipient beds immediately before transplantation, we next examined CD11 antigen-like family member B (CD11b)-positive macrophage infiltration into inflamed corneas in mice treated with NP2-amiR.
Gene_expression (expression) of NP2 in corneas associated with injury
4) Confidence 0.78 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0.31 Pain Relevance 0.04
These data confirmed that the intrastromal delivery of NP2-amiR resulted in reduced NP2 expression.


Gene_expression (expression) of NP2
5) Confidence 0.78 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0.14 Pain Relevance 0
These DCs were immature (major histocompatability complex class II(low)) and expressed vascular endothelial growth factor receptor 2.
Gene_expression (expressed) of vascular endothelial growth factor receptor 2 in DCs
6) Confidence 0.69 Published 2008 Journal FASEB J. Section Abstract Doc Link 17873101 Disease Relevance 1.40 Pain Relevance 0.42
The cells were then transfected with NP2-amiR or neg-amiR using Lipofectamine PLUS reagent (Invitrogen) and selected with 5 ug/ml blasticidin (Invitrogen).
Gene_expression (transfected) of NP2-amiR
7) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0 Pain Relevance 0
NP2 ELISA showed that NP2 concentration was 591.14±57.51 pg/ml in saline control mice, 605.58±52.58 pg/ml in neg-amiR treated mice (p=0.606, compared to control), and 372.62±50.89 pg/ml in mice injected with NP2-amiR (p=0.031, compared to control, Figure 1D).
Gene_expression (concentration) of NP2
8) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0.06 Pain Relevance 0
Intrastromal delivery of NP2-targeting amiRNA has no effect on cell infiltration
Gene_expression (delivery) of NP2
9) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0.30 Pain Relevance 0.03
Intrastromal delivery of NP2-targeting amiRNA inhibits corneal lymphangiogenesis
Gene_expression (delivery) of NP2
10) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0.15 Pain Relevance 0
Determination of NP2 levels by ELISA
Gene_expression (levels) of NP2
11) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0 Pain Relevance 0
NP2 ELISA showed that NP2 concentration was 591.14±57.51 pg/ml in saline control mice, 605.58±52.58 pg/ml in neg-amiR treated mice (p=0.606, compared to control), and 372.62±50.89 pg/ml in mice injected with NP2-amiR (p=0.031, compared to control, Figure 1D).
Gene_expression (injected) of NP2-amiR
12) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0.12 Pain Relevance 0
VEGF-C-induced NP2 expression at both mRNA and protein levels was significantly suppressed by NP2 amiRNA in mouse lymphatic endothelial cells.
Gene_expression (expression) of NP2 in endothelial cells
13) Confidence 0.60 Published 2010 Journal Molecular Vision Section Abstract Doc Link PMC2994732 Disease Relevance 0.13 Pain Relevance 0.03
Mouse lymphatic endothelial cells were transfected with the plasmid expressing artificial microRNA (amiRNA) against mouse NP2, and the down-regulation of VEGF-C-induced NP2 expression by NP2 amiRNA was evaluated by real-time PCR and western blot assays.
Gene_expression (expression) of NP2 in endothelial cells
14) Confidence 0.60 Published 2010 Journal Molecular Vision Section Abstract Doc Link PMC2994732 Disease Relevance 0.17 Pain Relevance 0.04
Western blot assay demonstrated that NP2-amiR but not negative control neg-amiR specially inhibited NP2 expression in mouse LECs stimulated by VEGF-C (Figure 1B).
Gene_expression (expression) of NP2
15) Confidence 0.60 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0 Pain Relevance 0
NP2-targeting amiRNA suppresses VEGF-C-induced NP2 expression in mouse LECs
Gene_expression (expression) of NP2
16) Confidence 0.60 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0 Pain Relevance 0
The following primers were used for real-time PCR: NP2, 5?
Gene_expression (used) of NP2
17) Confidence 0.59 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0 Pain Relevance 0
Intrastromal delivery of NP2-targeting amiRNA suppresses corneal NP2 expression
Gene_expression (expression) of NP2
18) Confidence 0.53 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2994732 Disease Relevance 0 Pain Relevance 0
Rubrospinal neurons express Npn-2 but not Npn-1.
Neg (not) Gene_expression (express) of Npn-2 in neurons
19) Confidence 0.31 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC2841193 Disease Relevance 0.25 Pain Relevance 0.08
AAV1-mediated expression of a control shRNA and a Npn-2-shRNA in the red nucleus resulted in an adverse tissue response, including neuronal cell degeneration.
Gene_expression (expression) of Npn-2 in neuronal
20) Confidence 0.31 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC2841193 Disease Relevance 0.24 Pain Relevance 0

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