INT150547

Context Info Confidence 0.79 First Reported 2008 Last Reported 2010 Negated 0 Speculated 1 Reported most in Body Documents 2 Total Number 8 Disease Relevance 4.92 Pain Relevance 2.30

cytosol (MAP2K3) nucleoplasm (MAP2K3) signal transduction (MAP2K3)

Anatomy Link Frequency chondrocytes 6 MAP2K3 (Homo sapiens)

Pain Link Frequency Relevance Heat Osteoarthritis 721 99.44 cytokine 56 85.40 Inflammation 114 10.00 Arthritis 56 5.00 metalloproteinase 42 5.00 rheumatoid arthritis 28 5.00 Pain 14 5.00 Inflammatory stimuli 7 5.00 Immobilon 7 5.00 imagery 7 5.00

Disease Link Frequency Relevance Heat Osteoarthritis 728 99.44 Targeted Disruption 21 95.28 Stress 8 89.36 Apoptosis 10 67.08 Arthritis 63 11.84 INFLAMMATION 99 5.00 Disease 35 5.00 Rheumatoid Arthritis 28 5.00 Pain 7 5.00 Hypertrophy 7 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Sevoflurane exposure also induced phosphorylation of apoptosis signal-regulating kinase-1 (ASK1), MAP kinase kinases 3 and 6 (MKK3/MKK6), and ATF-2.

Phosphorylation (phosphorylation) of MKK3

1) Confidence 0.79 Published 2008 Journal Anesth. Analg. Section Body Doc Link 18349187 Disease Relevance 0.07 Pain Relevance 0

Our results indicated that although both MKK3 and MKK6 were constitutively phosphorylated in the human OA chondrocytes (probably refecting the pre-activated state), but the effect of IL-1?

Phosphorylation (phosphorylated) of MKK3 in chondrocytes associated with osteoarthritis

2) Confidence 0.76 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.61 Pain Relevance 0.30

-induced phosphorylation of both MKK3 and MKK6 in a dose dependent manner with the maximum inhibitory effect of PE being at 50 ?

Phosphorylation (phosphorylation) of MKK3

3) Confidence 0.76 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.91 Pain Relevance 0.41

was more pronounced on the phosphorylation of MKK3 as compared to MKK6 suggesting that MKK3 may be the primary kinase involved in the activation of p38-MAPK in human OA chondrocytes.

Phosphorylation (phosphorylation) of MKK3 in chondrocytes associated with osteoarthritis

4) Confidence 0.76 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.60 Pain Relevance 0.30

Higher concentrations produced no additional suppression of phosphorylation of either MKK3 or MKK6 in human OA chondrocytes.

Phosphorylation (phosphorylation) of MKK3 in chondrocytes associated with osteoarthritis

5) Confidence 0.76 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 1.01 Pain Relevance 0.43

Our results show that both MKK3 and MKK6 were constitutively phosphorylated in the primary human OA chondrocytes but the intensity of MKK3 phosphorylation was significantly enhanced (P < 0.05) after stimulation with IL-1?

Phosphorylation (phosphorylated) of MKK3 in chondrocytes associated with osteoarthritis

6) Confidence 0.59 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.60 Pain Relevance 0.30

Our results show that both MKK3 and MKK6 were constitutively phosphorylated in the primary human OA chondrocytes but the intensity of MKK3 phosphorylation was significantly enhanced (P < 0.05) after stimulation with IL-1?

Phosphorylation (phosphorylation) of MKK3 in chondrocytes associated with osteoarthritis

7) Confidence 0.59 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.61 Pain Relevance 0.31

We then used these antibodies to determine the expression and phosphorylation of MKK3 and MKK6 proteins in human OA chondrocytes.

Spec (determine) Phosphorylation (phosphorylation) of MKK3 in chondrocytes associated with osteoarthritis

8) Confidence 0.59 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.51 Pain Relevance 0.25

General Comments

This test has worked.