INT155680

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Context Info
Confidence 0.66
First Reported 2006
Last Reported 2010
Negated 0
Speculated 2
Reported most in Body
Documents 22
Total Number 24
Disease Relevance 6.76
Pain Relevance 7.23

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

transport (Slc1a3) plasma membrane (Slc1a3)
Anatomy Link Frequency
whisker 3
neuronal 3
retina 2
brain 2
astrocyte 2
Slc1a3 (Mus musculus)
Pain Link Frequency Relevance Heat
Glutamate 623 100.00 Very High Very High Very High
nMDA receptor 19 100.00 Very High Very High Very High
excitatory amino acid 12 100.00 Very High Very High Very High
Morphine 12 99.20 Very High Very High Very High
Physical dependence 3 98.74 Very High Very High Very High
primary somatosensory cortex 10 98.56 Very High Very High Very High
depression 19 96.48 Very High Very High Very High
Anterior cingulate cortex 2 95.52 Very High Very High Very High
Somatosensory cortex 30 95.28 Very High Very High Very High
Glutamate receptor 35 93.72 High High
Disease Link Frequency Relevance Heat
Retina Disease 180 100.00 Very High Very High Very High
Congenital Anomalies 9 99.92 Very High Very High Very High
Injury 293 99.78 Very High Very High Very High
Penetrating Head Injuries 25 99.70 Very High Very High Very High
Stab Wounds 75 99.64 Very High Very High Very High
Brain Injury 50 99.64 Very High Very High Very High
Neurological Disease 1 98.80 Very High Very High Very High
Drug Dependence 3 98.74 Very High Very High Very High
Suicidal Behaviour 1 97.52 Very High Very High Very High
Targeted Disruption 471 97.28 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
We also measured the expression levels (Figure 4C) of six genes in addition to Clrn1 and Thy1: cFos, Slc1a3, Grm1, Rho, Rp1, and Vim.
Gene_expression (expression) of Slc1a3
1) Confidence 0.66 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2719914 Disease Relevance 0.10 Pain Relevance 0.04
When compared to expression levels in the WT animal, the loss of photoreceptors resulted in reduced expression of the immediate early gene transcription factor cFos, as well as Slc1a3 and Grm1, a glutamate transporter and receptor, respectively.
Gene_expression (expression) of Slc1a3 in photoreceptors associated with glutamate
2) Confidence 0.66 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2719914 Disease Relevance 0.09 Pain Relevance 0.05
Finally, Vim mRNA is expressed at a roughly similar level, while significantly fewer cFos, Slc1a3, and Grm1 transcripts are expressed in the WT mouse retina.
Gene_expression (expressed) of Slc1a3 in retina
3) Confidence 0.66 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2719914 Disease Relevance 0.07 Pain Relevance 0
On a longer time scale, however, the expression levels of GLT1 and GLAST have been shown, in vitro, to depend on the level of neuronal activity [14].
Gene_expression (expression) of GLAST in neuronal
4) Confidence 0.63 Published 2006 Journal PLoS Biology Section Body Doc Link PMC1609127 Disease Relevance 0.08 Pain Relevance 0.35
All the evidence supports the idea that regulating the expression of GLT1 and GLAST during a period of increased glutamate release, e.g., during increased sensory stimulation, is necessary for avoiding any effects of glutamate build-up.
Gene_expression (expression) of GLAST associated with glutamate
5) Confidence 0.63 Published 2006 Journal PLoS Biology Section Body Doc Link PMC1609127 Disease Relevance 0.09 Pain Relevance 0.34
Western blot analysis (Figure 1C and 1D) shows that after 24 h of whisker stimulation, GLAST and GLT1 expression increased 2.46-fold and 2.65-fold of the unstimulated value (GLAST: 246 ± 20.9% mean ± standard deviation [SD]; GLT1: 265 ± 38.1%; p < 0.01, Tukey honestly significant difference [HSD]).
Gene_expression (expression) of GLAST in whisker
6) Confidence 0.63 Published 2006 Journal PLoS Biology Section Body Doc Link PMC1609127 Disease Relevance 0 Pain Relevance 0.07
Using Western blot analysis on single barrel columns corresponding to the stimulated whisker, we found that expression levels of GLT1 and GLAST were significantly increased.
Gene_expression (expression) of GLAST in whisker
7) Confidence 0.55 Published 2006 Journal PLoS Biology Section Body Doc Link PMC1609127 Disease Relevance 0.15 Pain Relevance 0.27
GLT1, GLAST, and EAAC1 levels were normalized to actin.


Gene_expression (levels) of GLAST
8) Confidence 0.54 Published 2006 Journal PLoS Biology Section Body Doc Link PMC1609127 Disease Relevance 0 Pain Relevance 0.05
Additionally, staining of P7 mouse retina shows co-labeling of Clrn1 ISH with a Glast antibody (Figure S2).
Gene_expression (antibody) of Glast in retina
9) Confidence 0.50 Published 2009 Journal PLoS Genetics Section Body Doc Link PMC2719914 Disease Relevance 0 Pain Relevance 0
Similarly, GLT-1 and GLAST mRNA expression are induced after cultured astrocytes are physically traumatized [77,78].
Gene_expression (expression) of GLAST mRNA in astrocytes
10) Confidence 0.49 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1533808 Disease Relevance 0.69 Pain Relevance 0.23
Here we demonstrate that a penetrating brain injury increases the expression of GLT-1 and GLAST.
Gene_expression (expression) of GLAST in brain associated with penetrating head injuries
11) Confidence 0.49 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1533808 Disease Relevance 0.56 Pain Relevance 0.26
Thus, a neocortical stab wound injury induces the expression of GLT-1, GLAST, the GS, and S-100B, but our data indicate that this induction is independent of IL-1R1.
Gene_expression (expression) of GLAST associated with injury and stab wounds
12) Confidence 0.49 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1533808 Disease Relevance 0.80 Pain Relevance 0.15
This function of the astrocyte is dependent on its positioning around the synapse, as well as on the level of expression of its high-affinity glutamate transporters, GLT1 and GLAST.
Gene_expression (expression) of GLAST in astrocyte associated with glutamate
13) Confidence 0.48 Published 2006 Journal PLoS Biology Section Abstract Doc Link PMC1609127 Disease Relevance 0 Pain Relevance 0.21
Using mice, we found that 24 h of whisker stimulation elicited a 2-fold increase in the expression of GLT1 and GLAST in the corresponding cortical column of the barrel cortex.
Gene_expression (expression) of GLAST in barrel cortex
14) Confidence 0.48 Published 2006 Journal PLoS Biology Section Abstract Doc Link PMC1609127 Disease Relevance 0 Pain Relevance 0.26
On a longer time scale, however, the expression levels of GLT1 and GLAST have been shown, in vitro, to depend on the level of neuronal activity [14].
Gene_expression (levels) of GLAST in neuronal
15) Confidence 0.48 Published 2006 Journal PLoS Biology Section Body Doc Link PMC1609127 Disease Relevance 0.08 Pain Relevance 0.35
To assess the functional state of astrocytes after traumatic brain injury, we analyzed the expression of two glutamate transporters, glutamate aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1/EAAT2), the glutamate transaminase, glutamine synthetase (GS) and the calcium regulatory protein S-100B.
Spec (analyzed) Gene_expression (expression) of GLAST in brain associated with glutamate and brain injury
16) Confidence 0.43 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1533808 Disease Relevance 0.64 Pain Relevance 0.24
Our results show that in both WT and receptor-null mice, stab wound injury increased GLAST, GLT-1, GS and S-100B protein expression at 3 day post injury by 8, 6, 4 and 12 fold, respectively (Fig. 7).
Gene_expression (expression) of GLAST associated with injury and stab wounds
17) Confidence 0.43 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1533808 Disease Relevance 0.44 Pain Relevance 0.28
This study shows that an increase in sensory activity increases the expression of the astrocytic glutamate transporters GLT1 and GLAST in the corresponding region of the primary somatosensory cortex, without affecting the neuronal glutamate transporter EAAC1.
Gene_expression (expression) of GLAST in neuronal associated with primary somatosensory cortex and glutamate
18) Confidence 0.42 Published 2006 Journal PLoS Biology Section Body Doc Link PMC1609127 Disease Relevance 0.06 Pain Relevance 0.19
Using Western blot analysis on single barrel columns corresponding to the stimulated whisker, we found that expression levels of GLT1 and GLAST were significantly increased.
Gene_expression (levels) of GLAST in whisker
19) Confidence 0.42 Published 2006 Journal PLoS Biology Section Body Doc Link PMC1609127 Disease Relevance 0.15 Pain Relevance 0.27
Here we found that the expression of excitatory amino acid transporter 1 and 2 (GLAST, GLT1) was significantly decreased in asymptomatic and symptomatic A53T mice (Figs. 4O-P), indicating that excitotoxicity may occur in A53T mice.


Gene_expression (expression) of GLAST associated with targeted disruption and excitatory amino acid
20) Confidence 0.41 Published 2010 Journal Mol Brain Section Body Doc Link PMC2873589 Disease Relevance 0.85 Pain Relevance 0.38

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