INT158269

Context Info Confidence 0.76 First Reported 2009 Last Reported 2010 Negated 2 Speculated 0 Reported most in Body Documents 18 Total Number 23 Disease Relevance 2.06 Pain Relevance 1.20

transport (Trpm5) plasma membrane (Trpm5) biological_process (Trpm5)

Anatomy Link Frequency taste receptor cells 2 epithelial cells 1 taste buds 1 papillae 1 olfactory epithelium 1 Trpm5 (Mus musculus)

Pain Link Frequency Relevance Heat TRP channel 89 100.00 fifth nerve 34 99.68 imagery 265 99.46 Nerve growth factor 6 83.60 Mechanosensation 6 83.12 substance P 66 78.20 tetrodotoxin 1 74.72 Action potential 1 70.60 qutenza 135 69.88 c fibre 6 63.88

Disease Link Frequency Relevance Heat Targeted Disruption 274 99.42 Taste Disorders 1 90.24 Adhesions 4 87.24 Loss Of Sense Of Smell 24 83.40 Attention Deficit Hyperactivity Disorder 6 68.92 Overactive Bladder 6 68.36 Brain Injury 12 25.08 Hypersensitivity 8 22.16 Apoptosis 12 10.60 Death 18 8.92

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Imaging studies on heterologously expressed TRPM5 and TRPV1 channels are consistent with the behavioral experiments.

Gene_expression (expressed) of TRPM5 associated with imagery

1) Confidence 0.76 Published 2009 Journal J. Neurosci. Section Abstract Doc Link 19244541 Disease Relevance 0.26 Pain Relevance 0.12

TRPM5 is not expressed in the trigeminal nerve fibers innervating the SCCs.

Neg (not) Gene_expression (expressed) of TRPM5 in trigeminal nerve associated with fifth nerve

2) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2912856 Disease Relevance 0.30 Pain Relevance 0.09

These SCCs express transient receptor potential channel M5 (TRPM5) and the phospholipase C (PLC) ?

Gene_expression (express) of TRPM5 associated with trp channel

3) Confidence 0.76 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2912856 Disease Relevance 0.09 Pain Relevance 0.09

Previously, when we studied the TRPM5 expression in the olfactory sensory neurons [37], [38] and in SCCs of the respiratory epithelium [35], we noticed numerous TRPM5-expressing SCCs in the VNO entrance duct, the only pathway for stimulus fluids to enter the VNO.

Gene_expression (expression) of TRPM5 in duct

4) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2912856 Disease Relevance 0 Pain Relevance 0.04

The expression of TRPM5 and ?

Gene_expression (expression) of TRPM5

5) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2912856 Disease Relevance 0 Pain Relevance 0.03

Further, we have shown that PLC is involved in both Ca2+ responses in isolated SCCs and in the regulation of stimulus access and that TRPM5 expressed in the SCCs is especially important for limiting the amount of bitter compounds entering the VNOs.

Gene_expression (expressed) of TRPM5

6) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2912856 Disease Relevance 0 Pain Relevance 0

Because TRPM5 and PLC are also expressed in the main olfactory epithelium [37], [38], [50], we determined whether the regulation indeed is mediated by SCCs of the VNO.

Gene_expression ( also exp) of TRPM5 in olfactory epithelium

7) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2912856 Disease Relevance 0.16 Pain Relevance 0

We utilized the GFP expression in both TRPM5-GFP and ChAT(BAC)-eGFP mice to identify SCCs isolated from VNO tissues in Ca2+-imaging experiments.

Gene_expression (expression) of TRPM5-GFP associated with imagery

8) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2912856 Disease Relevance 0.08 Pain Relevance 0.05

In the main OE, TRPM5 was detected in solitary epithelial cells.

Gene_expression (detected) of TRPM5 in epithelial cells

9) Confidence 0.74 Published 2010 Journal Acta Oto-Laryngologica Section Body Doc Link PMC2981077 Disease Relevance 0.08 Pain Relevance 0.06

Expression of TRPV1, 2, 3, and 4, and TRPM5 has also been demonstrated by immunohistochemistry [3,6].

Gene_expression (Expression) of TRPM5

10) Confidence 0.74 Published 2010 Journal Acta Oto-Laryngologica Section Body Doc Link PMC2981077 Disease Relevance 0.08 Pain Relevance 0.16

In the pathological condition, the expression of TRPV3, TRPV4, TRPM5, and TRPA1 was markedly reduced and recovery took a long time.

Gene_expression (expression) of TRPM5

11) Confidence 0.74 Published 2010 Journal Acta Oto-Laryngologica Section Body Doc Link PMC2981077 Disease Relevance 0 Pain Relevance 0.13

Intracellular Ca(2+) and TRPM5-mediated membrane depolarization produce ATP secretion from taste receptor cells.

Gene_expression (produce) of TRPM5 in taste receptor cells

12) Confidence 0.67 Published 2010 Journal J. Physiol. (Lond.) Section Title Doc Link 20498227 Disease Relevance 0.07 Pain Relevance 0.11

These SCCs express transient receptor potential channel M5 (TRPM5) and the phospholipase C (PLC) ?

Gene_expression (express) of transient receptor potential channel M5 associated with trp channel

13) Confidence 0.66 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2912856 Disease Relevance 0.09 Pain Relevance 0.09

There was no significant difference between the wild type and TRPM5 knockout mice, suggesting functional expression of TRPM5 in SCCs is not critical for detecting Na+ salt or volatile compounds present in urinary samples.

Gene_expression (expression) of TRPM5 associated with targeted disruption

14) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2912856 Disease Relevance 0.18 Pain Relevance 0

Since TRPM5 is only expressed in SCCs, not in the trigeminal fibers, our results strongly demonstrate the role of SCCs and TRPM5 in detecting bitter fluid constituents to limit their access to VNOs.

Neg (not) Gene_expression (expressed) of TRPM5

15) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2912856 Disease Relevance 0.18 Pain Relevance 0.03

Recently, TRPM5 was shown to be enriched in taste receptor cells, where it is essential for the perception of sweet, bitter, and umami taste compounds.

Gene_expression (enriched) of TRPM5 in taste receptor cells

16) Confidence 0.64 Published 2010 Journal Acta Oto-Laryngologica Section Body Doc Link PMC2981077 Disease Relevance 0.23 Pain Relevance 0.15

An anti-TRPM5 antibody positively immunolabeled these cells, confirming the expression of TRPM5 (Fig. 1E).

Gene_expression (expression) of TRPM5

17) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2912856 Disease Relevance 0.05 Pain Relevance 0

In the pathological condition, the expression of TRPV3, TRPV4, TRPM5, and TRPA1 was markedly reduced and took a long time to recover.

Gene_expression (expression) of TRPM5

18) Confidence 0.58 Published 2010 Journal Acta Oto-Laryngologica Section Abstract Doc Link PMC2981077 Disease Relevance 0 Pain Relevance 0.04

Furthermore, TRPM5 was detected in sensory epithelia of the VNO [13], in the apical part of the cell, and in the present study, also in the ORNs, especially on the apical surface.

Gene_expression (detected) of TRPM5

19) Confidence 0.58 Published 2010 Journal Acta Oto-Laryngologica Section Body Doc Link PMC2981077 Disease Relevance 0.07 Pain Relevance 0.03

Because IP3R3 was coexpressed with Trpm5 in taste buds (Pérez et al. 2002), we predicted that Lrmp/Jaw1 may colocalize and interact with IP3R3.

Gene_expression (coexpressed) of Trpm5 in taste buds

20) Confidence 0.55 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

General Comments

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