INT158416

Context Info Confidence 0.67 First Reported 2004 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 19 Total Number 19 Disease Relevance 5.32 Pain Relevance 4.18

nucleoplasm (Mapk1) mitochondrion (Mapk1) protein complex (Mapk1) cytoplasm (Mapk1) cytosol (Mapk1) signal transduction (Mapk1)

Anatomy Link Frequency band 2 neuronal 1 stromal cells 1 spinal cord 1 nucleus 1 Mapk1 (Mus musculus)

Pain Link Frequency Relevance Heat c fibre 3 99.44 Spinal cord 50 99.28 Dorsal horn 10 98.94 antagonist 138 98.52 Hippocampus 12 98.44 long-term potentiation 104 97.64 Neuropathic pain 68 95.12 tetrodotoxin 2 94.32 agonist 56 91.28 Traumatic nerve injury 3 90.12

Disease Link Frequency Relevance Heat Apoptosis 70 99.20 Disease 22 98.58 Nervous System Injury 27 98.36 Cancer 153 97.04 Retinal Neovascularization 4 95.68 Neuropathic Pain 98 95.12 Nociception 28 91.00 Hypersensitivity 21 88.88 Cv Unclassified Under Development 1 88.88 INFLAMMATION 126 87.28

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

In addition to different types of noxious stimuli, high intensity electrical stimulation of C-fibers also activates ERK in the spinal cord dorsal horn, suggesting that C-fiber recruitment is crucial for release of transmitters that activate ERK centrally in the spinal cord [6,10].

Localization (release) of ERK in spinal cord associated with c fibre, dorsal horn and spinal cord

1) Confidence 0.67 Published 2006 Journal Mol Pain Section Body Doc Link PMC1382249 Disease Relevance 0.50 Pain Relevance 0.67

evidence was published on the combined use of ERK cascade inhibition and PPAR?

Localization (use) of ERK cascade

2) Confidence 0.59 Published 2008 Journal PPAR Research Section Body Doc Link PMC2440494 Disease Relevance 0.65 Pain Relevance 0.11

The activation of ERK pathway and subsequent translocation of NF-kappaB was possibly necessary for the up-regulation TRPC6 induced by Ang II.

Localization (translocation) of ERK

3) Confidence 0.59 Published 2009 Journal Exp. Biol. Med. (Maywood) Section Abstract Doc Link 19546355 Disease Relevance 0.52 Pain Relevance 0.08

Considering the importance of ERK in regulating gene expression, the ERK activation may be required for L-LTP.

Localization (importance) of ERK associated with long-term potentiation

4) Confidence 0.58 Published 2007 Journal Mol Pain Section Body Doc Link PMC2245920 Disease Relevance 0 Pain Relevance 0.84

The signalling pathways were pharmacologically investigated with the pre-administration of adenylyl cyclase (AC), cAMP-dependent protein kinase (PKA), protein kinase Cepsilon (PKCepsilon), and the extracellular signal-related kinase (ERK) inhibitors.

Localization (administration) of ERK

5) Confidence 0.47 Published 2009 Journal Life Sci. Section Body Doc Link 19896488 Disease Relevance 0 Pain Relevance 0

The signalling pathways were pharmacologically investigated with the pre-administration of adenylyl cyclase (AC), cAMP-dependent protein kinase (PKA), protein kinase Cepsilon (PKCepsilon), and the extracellular signal-related kinase (ERK) inhibitors.

Localization (administration) of extracellular signal-related kinase

6) Confidence 0.47 Published 2009 Journal Life Sci. Section Body Doc Link 19896488 Disease Relevance 0 Pain Relevance 0

In normal noncancerous tissue from radical prostatectomy specimens, immunohistochemistry localizes ERK to the cytoplasm of most cells of the prostate including the epithelial, basal, and stromal cells [1,2].

Localization (localizes) of ERK in stromal cells

7) Confidence 0.40 Published 2004 Journal Cell Commun Signal Section Body Doc Link PMC449737 Disease Relevance 0.19 Pain Relevance 0

Our observation that active ERK was localized to angiogenic buds and neovascular outgrowths indicates that altered MKK signaling also may be a factor in retinal neovascularization.

Localization (localized) of ERK associated with retinal neovascularization

8) Confidence 0.36 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2737623 Disease Relevance 0.51 Pain Relevance 0.08

The signal by which nerve injury or disease may lead to microglial activation is unclear, but may relate to acute release of ERK, an enzyme that plays a critical role in intracellular signal transduction and neuronal plasticity.

Localization (release) of ERK in neuronal associated with nervous system injury and disease

9) Confidence 0.28 Published 2010 Journal Mol Pain Section Body Doc Link PMC2845559 Disease Relevance 1.32 Pain Relevance 0.83

Immunoblot analyses for angiotensin II, AT1R, synaptophysin, and phosphorylated forms of ERK, Akt, and STAT3.

Localization (forms) of ERK

10) Confidence 0.26 Published 2008 Journal Diabetes Section Body Doc Link PMC2494692 Disease Relevance 0.08 Pain Relevance 0.08

ZnR activation induced intracellular release of Ca(2+), as well as phosphorylation of extracellular-regulated kinase and Ca(2+)/calmodulin kinase II.

Localization (release) of extracellular-regulated kinase

11) Confidence 0.22 Published 2009 Journal J. Neurosci. Section Abstract Doc Link 19261885 Disease Relevance 0 Pain Relevance 0.23

and c-Jun (a phosphorylation target of ERK and JNK).

Localization (target) of ERK

12) Confidence 0.18 Published 2004 Journal Journal of Biomedicine and Biotechnology Section Body Doc Link PMC1082887 Disease Relevance 0.17 Pain Relevance 0

Normalization of p-ERK and t-ERK were also conducted against beta-actin, then p-ERK data were quantified as relative intensity of band divided by intensity of t-ERK.

Localization (Normalization) of ERK in band

13) Confidence 0.15 Published 2009 Journal Mol Pain Section Body Doc Link PMC2704199 Disease Relevance 0 Pain Relevance 0

Normalization of p-ERK and t-ERK were also conducted against beta-actin, then p-ERK data were quantified as relative intensity of band divided by intensity of t-ERK.

Localization (Normalization) of ERK in band

14) Confidence 0.15 Published 2009 Journal Mol Pain Section Body Doc Link PMC2704199 Disease Relevance 0 Pain Relevance 0

We have now demonstrated that expression of c-Myc requires phosphorylation and nuclear translocation of ERK, which results in phosphorylation of c-Fos and formation of a specific activator protein (AP)-1 complex.

Localization (translocation) of ERK

15) Confidence 0.13 Published 2010 Journal J. Biol. Chem. Section Abstract Doc Link 20410304 Disease Relevance 0.75 Pain Relevance 0.13

-induced elevation in COX-2 Luciferase (Fig. 5C) and mRNA expression (Fig. 5D) was significantly inhibited by co-treatment of cells with the FP receptor antagonist (AL8810), and chemical inhibitors of PLC (U73122), PKA (4C3MQ), EGFR kinase (AG1478) and ERK1/2 kinase (PD98059), but not the EP2 receptor antagonist (AH6809), EP4 receptor antagonist (ONOAE2227) or PKC inhibitor (GF109203x; Fig. 5C and D, P < 0.05).

Localization (kinase) of ERK1/2 associated with antagonist

16) Confidence 0.12 Published 2008 Journal Molecular and Cellular Endocrinology Section Body Doc Link PMC2694994 Disease Relevance 0 Pain Relevance 0.35

The PGE2-induced elevation in COX-2 Luciferase (Fig. 5A) and mRNA expression (Fig. 5B) was significantly reduced by treatment of FPS cells with the FP receptor antagonist (AL8810), EP2 receptor antagonist (AH6809), EP4 receptor antagonist (ONOAE2227), and chemical inhibitors of PLC (U73122), PKA (4C3MQ), EGFR kinase (AG1478) and ERK1/2 kinase (PD98059), but not the PKC inhibitor (GF109203x; P < 0.05).

Localization (kinase) of ERK1/2 associated with antagonist

17) Confidence 0.12 Published 2008 Journal Molecular and Cellular Endocrinology Section Body Doc Link PMC2694994 Disease Relevance 0 Pain Relevance 0.36

Activated MEK subsequently phosphorylates ERK, which translocates to the nucleus where it activates multiple transcription factors.

Localization (translocates) of ERK in nucleus

18) Confidence 0.08 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991030 Disease Relevance 0.64 Pain Relevance 0.39

Additional antibodies included rabbit Erk 1/2 (Cell Signaling Technologies) and anti-phospho ERK1/2 purchased from Promega Corporation (Madison, WI).

Localization (purchased) of ERK1/2

19) Confidence 0.07 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2837737 Disease Relevance 0 Pain Relevance 0.03

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