INT159141

Context Info Confidence 0.57 First Reported 2007 Last Reported 2009 Negated 0 Speculated 0 Reported most in Body Documents 7 Total Number 7 Disease Relevance 5.44 Pain Relevance 0.62

Golgi apparatus (Tjp1) plasma membrane (Tjp1) nucleus (Tjp1) cytoplasm (Tjp1)

Anatomy Link Frequency CAR 2 colon 2 lung 2 Tjp1 (Mus musculus)

Pain Link Frequency Relevance Heat Nicotine 121 99.30 anesthesia 12 91.28 Crohn's disease 8 81.20 noradrenaline 6 43.28 cytokine 4 37.76 Inflammation 20 31.56 transdermal 8 5.00 Versed 4 5.00 antagonist 3 5.00 tetrodotoxin 2 5.00

Disease Link Frequency Relevance Heat Brain Injury 33 99.56 Injury 9 99.44 Lung Cancer 24 98.84 Weight Loss 4 98.02 Colitis 62 97.92 Sepsis 3 93.68 Adhesions 5 90.36 Targeted Disruption 75 88.96 Arrhythmia Under Development 19 86.72 Inflammatory Bowel Disease 34 85.36

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Expression of ZO-1 was decreased by 49% relative to sham animals (p < 0.02), whereas expression of occludin was decreased by 73% relative to sham animals (p < 0.001).

Negative_regulation (decreased) of Gene_expression (Expression) of ZO-1

1) Confidence 0.57 Published 2009 Journal J. Neurotrauma Section Abstract Doc Link 19344293 Disease Relevance 0.89 Pain Relevance 0.07

We hypothesized that expression of tight junction protein ZO-1 and occludin, responsible for intestinal architectural and functional integrity, will decrease following TBI and increase intestinal permeability.

Negative_regulation (decrease) of Gene_expression (expression) of ZO-1 associated with brain injury

2) Confidence 0.41 Published 2009 Journal J. Neurotrauma Section Abstract Doc Link 19344293 Disease Relevance 1.03 Pain Relevance 0.09

An increase in intestinal permeability corresponds with decreased expression of tight junction proteins ZO-1 and occludin following TBI.

Negative_regulation (decreased) of Gene_expression (expression) of ZO-1 associated with brain injury

3) Confidence 0.41 Published 2009 Journal J. Neurotrauma Section Abstract Doc Link 19344293 Disease Relevance 0.73 Pain Relevance 0.07

It is feasible that the reduction of DSS-mediated downregulation of ZO-1 expression by EcN treatment is one reason for the enhanced barrier stability.

Negative_regulation (downregulation) of Gene_expression (expression) of ZO-1

4) Confidence 0.30 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.54 Pain Relevance 0.04

However, administration of EcN in our mouse models not only diminished the clinical signs of colitis like colon shortening and weight loss, but also prevented an increase in intestinal permeability while concurrently minimizing the down-regulation of IEC ZO-1 expression.

Negative_regulation (regulation) of Gene_expression (expression) of IEC ZO-1 in colon associated with colitis and weight loss

5) Confidence 0.27 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.70 Pain Relevance 0.04

Moreover lung tumor sections from nicotine treated mice showed significantly reduced expression of ZO-1 upon immunostaining (Figure 5E & F).

Negative_regulation (reduced) of Gene_expression (expression) of ZO-1 in lung associated with lung cancer and nicotine

6) Confidence 0.15 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2759510 Disease Relevance 1.10 Pain Relevance 0.30

This includes reduced expression of ZO-1, an adaptor protein that binds both CAR and connexins (Fig. 6 A) (8, 21).

Negative_regulation (reduced) of Gene_expression (expression) of ZO-1 in CAR

7) Confidence 0.08 Published 2008 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2556793 Disease Relevance 0.44 Pain Relevance 0

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