INT159143

Context Info Confidence 0.42 First Reported 2009 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 14 Total Number 16 Disease Relevance 5.25 Pain Relevance 0.57

cytosol (Ocln) structural molecule activity (Ocln) plasma membrane (Ocln)

Anatomy Link Frequency keratinocytes 3 ileum 1 retinas 1 brain 1 Ocln (Mus musculus)

Pain Link Frequency Relevance Heat Inflammatory response 30 87.28 anesthesia 15 84.72 Inflammation 171 82.92 imagery 11 82.52 Neurotransmitter 2 65.00 cytokine 98 47.16 Kinase C 2 31.12 member 8 12 5.00 Central nervous system 9 5.00 Inflammatory mediators 9 5.00

Disease Link Frequency Relevance Heat Brain Injury 44 99.56 Body Weight 7 99.50 Aging 24 97.28 Injury 52 96.04 Dermatitis 36 94.56 Sepsis 4 93.68 INFLAMMATION 192 87.28 Brain Disease 4 87.08 Psoriasis 42 83.04 Metastasis 75 81.44

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

We hypothesized that expression of tight junction protein ZO-1 and occludin, responsible for intestinal architectural and functional integrity, will decrease following TBI and increase intestinal permeability.

Gene_expression (expression) of occludin associated with brain injury

1) Confidence 0.42 Published 2009 Journal J. Neurotrauma Section Abstract Doc Link 19344293 Disease Relevance 0.95 Pain Relevance 0.08

Expression of ZO-1 was decreased by 49% relative to sham animals (p < 0.02), whereas expression of occludin was decreased by 73% relative to sham animals (p < 0.001).

Gene_expression (expression) of occludin

2) Confidence 0.42 Published 2009 Journal J. Neurotrauma Section Abstract Doc Link 19344293 Disease Relevance 0.87 Pain Relevance 0.07

An increase in intestinal permeability corresponds with decreased expression of tight junction proteins ZO-1 and occludin following TBI.

Gene_expression (expression) of occludin associated with brain injury

3) Confidence 0.37 Published 2009 Journal J. Neurotrauma Section Abstract Doc Link 19344293 Disease Relevance 0.73 Pain Relevance 0.06

Whole ileum extracts were used to measure expression of tight junction proteins ZO-1 and occludin by Western blot.

Gene_expression (expression) of occludin in ileum

4) Confidence 0.37 Published 2009 Journal J. Neurotrauma Section Abstract Doc Link 19344293 Disease Relevance 1.06 Pain Relevance 0.08

Injected hAFSC expressed NPHS1, AQP2, PAX2, and OCLN, when compared to hAFSC before injection (Figure 4F).

Gene_expression (expressed) of OCLN

5) Confidence 0.36 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2827539 Disease Relevance 0 Pain Relevance 0

In contrast, expression of several claudins and of occludin was much lower in the epidermis and in cultured keratinocytes of K5-R1/R2 mice.

Gene_expression (expression) of occludin in keratinocytes

6) Confidence 0.33 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2845079 Disease Relevance 0.32 Pain Relevance 0.11

Immunoprecipitation of occludin, followed by western blotting for zo-1, revealed a decrease in association between these two proteins in sEng-expressing mice (Figure 3D).

Gene_expression (Immunoprecipitation) of occludin

7) Confidence 0.30 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2659748 Disease Relevance 0 Pain Relevance 0.03

In addition, we found that expression levels of claudins and occludin strongly declined in control mice after P18 (Fig.

Gene_expression (expression) of occludin

8) Confidence 0.29 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2845079 Disease Relevance 0.05 Pain Relevance 0

While there was no change in the levels of the tight junction proteins zo-1 and occludin in retinas from sEng-expressing mice (data not shown), there was a decrease in association between these proteins, a characteristic feature of tight junction disassembly [28].

Gene_expression (expressing) of occludin in retinas

9) Confidence 0.27 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2659748 Disease Relevance 0.07 Pain Relevance 0

EGF also induced their expression under these conditions (unpublished data), but it can obviously not compensate for the loss of FGF receptors because expression of claudin 1, claudin 3, and occludin was reduced in keratinocytes of K5-R1/R2 mice, which were cultured in the presence of EGF (Fig. 9 B).

Gene_expression (expression) of occludin in keratinocytes

10) Confidence 0.26 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2845079 Disease Relevance 0.06 Pain Relevance 0

To determine if the down-regulation of claudin/occludin expression is a cell-autonomous effect, we analyzed the expression of these tight junction components in immortalized keratinocytes.

Gene_expression (expression) of occludin in keratinocytes

11) Confidence 0.26 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2845079 Disease Relevance 0.23 Pain Relevance 0.03

S4 shows the time course of claudin-3, -8, and occludin expression during postnatal development.

Gene_expression (expression) of occludin

12) Confidence 0.26 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2845079 Disease Relevance 0.08 Pain Relevance 0

Concomitantly, expression of IL-1F8 was reduced by 30%, whereas no obvious change in the expression of claudin 1, claudin 3, or occludin was observed (unpublished data).

Gene_expression (expression) of occludin

13) Confidence 0.22 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2845079 Disease Relevance 0.17 Pain Relevance 0.04

Because PCB exposure does not alter mRNA levels of genes encoding for TJ proteins (Eum et al. 2008), our studies focused on the effects of PCBs on protein expression of claudin-5, occludin, and ZO-1.

Gene_expression (expression) of occludin

14) Confidence 0.11 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2854723 Disease Relevance 0 Pain Relevance 0

No alterations in the expression of occludin were detected after any PCB treatment.

Gene_expression (expression) of occludin

15) Confidence 0.11 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2854723 Disease Relevance 0 Pain Relevance 0

mol/kg body weight) differentially altered expression of the TJ proteins claudin-5, occludin, and zonula occludens-1 in brain capillaries.

Gene_expression (expression) of occludin in brain associated with body weight

16) Confidence 0.08 Published 2010 Journal Environ Health Perspect Section Abstract Doc Link PMC2854723 Disease Relevance 0.66 Pain Relevance 0.04

General Comments

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