INT159146

Context Info Confidence 0.44 First Reported 2007 Last Reported 2010 Negated 0 Speculated 1 Reported most in Body Documents 13 Total Number 14 Disease Relevance 6.91 Pain Relevance 0.45

Golgi apparatus (Tjp1) plasma membrane (Tjp1) nucleus (Tjp1) cytoplasm (Tjp1)

Anatomy Link Frequency colon 2 gut 2 Tjp1 (Mus musculus)

Pain Link Frequency Relevance Heat Inflammation 125 99.48 Crohn's disease 44 88.16 anesthesia 13 85.76 Inflammatory response 4 42.56 tolerance 1 37.32 Inflammatory mediators 6 17.36 Versed 22 5.00 cytokine 22 5.00 tetrodotoxin 11 5.00 Central nervous system 3 5.00

Disease Link Frequency Relevance Heat INFLAMMATION 123 99.48 Colitis 341 99.00 Brain Injury 11 98.64 Weight Loss 22 98.02 Sepsis 1 93.68 Inflammatory Bowel Disease 187 93.52 Recurrence 22 92.40 Disease 46 88.16 Injury 13 83.56 Infection 85 83.52

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

We hypothesized that expression of tight junction protein ZO-1 and occludin, responsible for intestinal architectural and functional integrity, will decrease following TBI and increase intestinal permeability.

Regulation (responsible) of Gene_expression (expression) of ZO-1 associated with brain injury

1) Confidence 0.44 Published 2009 Journal J. Neurotrauma Section Abstract Doc Link 19344293 Disease Relevance 0.95 Pain Relevance 0.09

To further analyze the up-regulated ZO-1 expression at protein level, isolated IECs were investigated by Western blotting.

Spec (analyze) Regulation (regulated) of Spec (analyze) Gene_expression (expression) of ZO-1

2) Confidence 0.43 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0 Pain Relevance 0

Thus, we could clearly demonstrate that colonization of gnotobiotic mice with EcN specifically up-regulates ZO-1 expression at the mRNA as well as at the protein level.

Regulation (regulates) of Gene_expression (expression) of ZO-1

3) Confidence 0.43 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0 Pain Relevance 0

Elevated ZO-1 expression after EcN treatment in experimental colitis

Regulation (after) of Gene_expression (expression) of ZO-1 associated with colitis

4) Confidence 0.32 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.48 Pain Relevance 0

The present study clearly demonstrates that EcN specifically up-regulates ZO-1 mRNA expression in IECs in vivo.

Regulation (regulates) of Gene_expression (expression) of ZO-1 mRNA

5) Confidence 0.32 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.47 Pain Relevance 0

However, administration of EcN in our mouse models not only diminished the clinical signs of colitis like colon shortening and weight loss, but also prevented an increase in intestinal permeability while concurrently minimizing the down-regulation of IEC ZO-1 expression.

Regulation (regulation) of Gene_expression (expression) of IEC ZO-1 in colon associated with colitis and weight loss

6) Confidence 0.28 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.70 Pain Relevance 0.04

This idea is supported by our in vitro observations of a cox-2 dependent increase in Zo-1 protein expression in MKN28 cells.

Regulation (dependent) of Gene_expression (expression) of Zo-1 protein

7) Confidence 0.24 Published 2009 Journal Mol Cancer Section Body Doc Link PMC2667483 Disease Relevance 0.22 Pain Relevance 0

This report is the first to demonstrate a direct influence of the therapeutic EcN on expression of the TJ associated molecule ZO-1 in vivo under healthy and inflammatory conditions.

Regulation (influence) of Gene_expression (expression) of ZO-1 associated with inflammation

8) Confidence 0.19 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.97 Pain Relevance 0.09

This report is the first to demonstrate a direct influence of the therapeutic EcN on expression of the TJ associated molecule ZO-1 in vivo under healthy and inflammatory conditions.

Regulation (under) of Gene_expression (expression) of ZO-1 associated with inflammation

9) Confidence 0.19 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.96 Pain Relevance 0.09

In addition, we observed an altered ZO-1 expression profile in IECs of mice with DSS-induced colitis.

Regulation (altered) of Gene_expression (expression) of ZO-1 associated with colitis

10) Confidence 0.19 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.75 Pain Relevance 0.08

This indicates a considerable association between the severity of colitis, as evidenced by increased gut permeability, and altered ZO-1 mRNA expression levels.

Regulation (altered) of Gene_expression (expression) of ZO-1 mRNA in gut associated with colitis

11) Confidence 0.19 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.62 Pain Relevance 0.04

First, EcN is capable of producing a specific up-regulation of ZO-1 expression in IECs of healthy gnotobiotic mice.

Regulation (regulation) of Gene_expression (expression) of ZO-1

12) Confidence 0.19 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.37 Pain Relevance 0

This study convincingly demonstrates that probiotic EcN is able to mediate up-regulation of ZO-1 expression in murine IECs and confer protection from the DSS colitis-associated increase in mucosal permeability to luminal substances.

Regulation (regulation) of Gene_expression (expression) of ZO-1 associated with colitis

13) Confidence 0.19 Published 2007 Journal PLoS ONE Section Abstract Doc Link PMC2110898 Disease Relevance 0.42 Pain Relevance 0.03

Because PCB exposure does not alter mRNA levels of genes encoding for TJ proteins (Eum et al. 2008), our studies focused on the effects of PCBs on protein expression of claudin-5, occludin, and ZO-1.

Regulation (effects) of Gene_expression (expression) of ZO-1

14) Confidence 0.05 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2854723 Disease Relevance 0 Pain Relevance 0

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