INT159899
From wiki-pain
|
|
|
|
|
Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Recordings in skin nerve preparations from TRPA1 deficient mice and their wild type littermates revealed that in the absence of TRPA1, C fiber and A? | |||||||||||||||
| |||||||||||||||
|
For example, it is possible that normally, TRPA1 activation near the epidermal surface of the skin leads to the release of signaling molecules from non-neuronal skin cells that potentiate the A-mechanoreceptors. | |||||||||||||||
| |||||||||||||||
|
Intragastric administration of TRPV1, TRPV3, TRPM8, and TRPA1 agonists modulates autonomic thermoregulation in different manners in mice. | |||||||||||||||
| |||||||||||||||
|
Methods: The localization of TRPV14, TRPM5 and 8, and TRPA1 in the OE of normal and methimazole-treated CBA/J mice was investigated by immunohistochemistry. | |||||||||||||||
| |||||||||||||||
|
The following primer pairs were used: TRPA1 (Forward: 5'-GGAGACCCTGCTTCACAGAG-3', Reverse: 5'-AGTGGAGAGCGTCCTTCAGA-3'), HPRT (Hypoxanthine phosphoribosyl transferase) (Forward: 5'-GGCCAGACTTTGTTGGATTTG-3', Reverse: 5'-TGCGCTCATCTTAGGCTTTGT-3')
In Situ Hybridization and Immunostaining | |||||||||||||||
| |||||||||||||||
|
Furthermore, concentrations below the threshold of activation sensitized the CGRP release evoked by noxious heat and acrolein, activators of TRPV1 and TRPA1 receptors, respectively. | |||||||||||||||
| |||||||||||||||
|
Alternatively, mechanical damage could indirectly activate TRPA1 through release of reactive compounds or intracellular calcium (both of which can directly activate mammalian TRPA1) [11,12,31]. | |||||||||||||||
| |||||||||||||||
|
To detect TRPV1, TRPM8, and TRPA1 mRNA expression in sensory neurons, TG and DRG tissues from control rats and mice were isolated for RNA extraction followed by RT-PCR and gel electrophoresis. | |||||||||||||||
| |||||||||||||||
|
These findings were based upon localization of mTRPA1 transcripts and protein in hair cells, deficits in hair cell function after inhibition of TRPA1 protein expression, and similar biophysical and pharmacological properties of heterologously expressed TRPA1 and the hair cell transduction channel. | |||||||||||||||
| |||||||||||||||
|
General Comments
This test has worked.