INT160264
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
The primer set for COX2, FN1 and VEGF was amplified using increasing numbers of cycles of 95°C for 15 sec, 58°C for 30 sec, 72°C for 10 sec, and a final extension of 72°C for 1 minute. | |||||||||||||||
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There was no difference in the localization of laminin or fibronectin between groups (laminin expression was reduced in the tamoxifen treated uteri). | |||||||||||||||
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There was no difference in the localisation of laminin or fibronectin between control and tamoxifen-treated groups. | |||||||||||||||
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Cell proliferation of smooth muscle cells cultured on different substrates, FN, LN, and Matrigel Basement Membrane Matrix was detected with 5-Bromo2? | |||||||||||||||
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-estradiol, in the meibomian gland include secreted acidic cysteine rich glycoprotein (regulates cell growth [71]), vascular endothelial growth factor A (promotes cell migration, among many other actions [71]), matrix metalloproteinase 3 (degrades fibronectin, laminin, gelatins and collagens [71]) and cathepsin K (degrades extracellular matrices [71]). | |||||||||||||||
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To determine whether the effect of LIMK1 siRNA on fibronectin fibrillogenesis was due to a decreased fibronectin secretion, we analyzed the amount of secreted fibronectin by western blotting (Figure 1F). | |||||||||||||||
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For secreted fibronectin, 72 h following transfection, cells were incubated with serum-free media for 24 h. | |||||||||||||||
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Consistent with the immunofluorescence data, treatment with 50 nM or 100 nM LIMK1 siRNA resulted in decreased fibronectin secretion into the culture; the reduction, up to 80%, was dose- dependent.
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To determine whether the effect of LIMK1 siRNA on fibronectin fibrillogenesis was due to a decreased fibronectin secretion, we analyzed the amount of secreted fibronectin by western blotting (Figure 1F). | |||||||||||||||
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LIMK1 siRNA inhibits fibronectin assembly and fibronectin secretion | |||||||||||||||
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Downregulation of LIMK1 in cultured corneal fibroblasts impaired fibronectin secretion and assembly, diminished actin polymerization and focal adhesion formation, and retarded cell migration. | |||||||||||||||
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General Comments
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