INT160264

Context Info Confidence 0.40 First Reported 2008 Last Reported 2010 Negated 0 Speculated 1 Reported most in Body Documents 10 Total Number 11 Disease Relevance 3.95 Pain Relevance 1.51

extracellular space (Fn1) cell adhesion (Fn1) proteinaceous extracellular matrix (Fn1) extracellular matrix organization (Fn1)

Anatomy Link Frequency smooth muscle cells 1 fibroblasts 1 meibomian gland 1 Fn1 (Mus musculus)

Pain Link Frequency Relevance Heat COX2 7 100.00 metalloproteinase 2 100.00 Inflammation 225 93.68 Dismenorea 12 93.24 Inflammatory response 55 81.60 fibrosis 73 65.76 ketamine 7 34.20 anesthesia 7 32.44 cytokine 7 5.00 chemokine 4 5.00

Disease Link Frequency Relevance Heat Disorders Of The Lacrimal System 85 99.82 Adhesions 67 97.72 Syndrome 2 96.00 INFLAMMATION 201 93.68 Dysmenorrhea 12 93.24 Ocular & Orbital Inflammation 78 89.76 Injury 85 84.96 Infection 96 72.68 Respiratory Syncytial Virus 99 71.96 Sprains And Strains 2 67.16

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

The primer set for COX2, FN1 and VEGF was amplified using increasing numbers of cycles of 95°C for 15 sec, 58°C for 30 sec, 72°C for 10 sec, and a final extension of 72°C for 1 minute.

Localization (set) of FN1 associated with cox2

1) Confidence 0.40 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2556398 Disease Relevance 0.41 Pain Relevance 0.13

There was no difference in the localization of laminin or fibronectin between groups (laminin expression was reduced in the tamoxifen treated uteri).

Localization (localization) of fibronectin

2) Confidence 0.35 Published 2009 Journal Reproduction Section Abstract Doc Link 19451194 Disease Relevance 0.28 Pain Relevance 0.35

There was no difference in the localisation of laminin or fibronectin between control and tamoxifen-treated groups.

Localization (localisation) of fibronectin

3) Confidence 0.33 Published 2010 Journal Reproduction Section Abstract Doc Link 20368191 Disease Relevance 0.53 Pain Relevance 0.40

Cell proliferation of smooth muscle cells cultured on different substrates, FN, LN, and Matrigel Basement Membrane Matrix was detected with 5-Bromo–2?

Localization (proliferation) of FN in smooth muscle cells

4) Confidence 0.24 Published 2008 Journal Mol Cell Biochem Section Body Doc Link PMC2758386 Disease Relevance 0 Pain Relevance 0

-estradiol, in the meibomian gland include secreted acidic cysteine rich glycoprotein (regulates cell growth [71]), vascular endothelial growth factor A (promotes cell migration, among many other actions [71]), matrix metalloproteinase 3 (degrades fibronectin, laminin, gelatins and collagens [71]) and cathepsin K (degrades extracellular matrices [71]).

Localization (secreted) of fibronectin in meibomian gland associated with disorders of the lacrimal system and metalloproteinase

5) Confidence 0.16 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2728565 Disease Relevance 0.94 Pain Relevance 0.19

To determine whether the effect of LIMK1 siRNA on fibronectin fibrillogenesis was due to a decreased fibronectin secretion, we analyzed the amount of secreted fibronectin by western blotting (Figure 1F).

Localization (secretion) of fibronectin

6) Confidence 0.14 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2576479 Disease Relevance 0.11 Pain Relevance 0

For secreted fibronectin, 72 h following transfection, cells were incubated with serum-free media for 24 h.

Localization (secreted) of fibronectin

7) Confidence 0.14 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2576479 Disease Relevance 0.12 Pain Relevance 0

Consistent with the immunofluorescence data, treatment with 50 nM or 100 nM LIMK1 siRNA resulted in decreased fibronectin secretion into the culture; the reduction, up to 80%, was dose- dependent.

Localization (secretion) of fibronectin

8) Confidence 0.14 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2576479 Disease Relevance 0.23 Pain Relevance 0

To determine whether the effect of LIMK1 siRNA on fibronectin fibrillogenesis was due to a decreased fibronectin secretion, we analyzed the amount of secreted fibronectin by western blotting (Figure 1F).

Spec (analyzed) Localization (secreted) of fibronectin

9) Confidence 0.14 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2576479 Disease Relevance 0.12 Pain Relevance 0

LIMK1 siRNA inhibits fibronectin assembly and fibronectin secretion

Localization (secretion) of fibronectin

10) Confidence 0.14 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2576479 Disease Relevance 0.30 Pain Relevance 0.12

Downregulation of LIMK1 in cultured corneal fibroblasts impaired fibronectin secretion and assembly, diminished actin polymerization and focal adhesion formation, and retarded cell migration.

Localization (secretion) of fibronectin in fibroblasts associated with adhesions

11) Confidence 0.12 Published 2008 Journal Molecular Vision Section Abstract Doc Link PMC2576479 Disease Relevance 0.92 Pain Relevance 0.33

General Comments

This test has worked.