INT162102

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Context Info
Confidence 0.72
First Reported 2009
Last Reported 2009
Negated 0
Speculated 0
Reported most in Body
Documents 3
Total Number 35
Disease Relevance 23.65
Pain Relevance 27.96

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytosol (Gch1) nucleolus (Gch1) nucleus (Gch1)
protein complex assembly (Gch1) protein complex (Gch1) cytoplasm (Gch1)
Anatomy Link Frequency
sciatic nerve 2
nerve 1
spinal cord 1
CSF 1
microglia 1
Gch1 (Rattus norvegicus)
Pain Link Frequency Relevance Heat
GCH1 1852 100.00 Very High Very High Very High
Inflammation 330 100.00 Very High Very High Very High
Sciatic nerve 495 99.60 Very High Very High Very High
Pain 1520 99.56 Very High Very High Very High
dorsal root ganglion 528 99.48 Very High Very High Very High
Spinal cord 202 99.48 Very High Very High Very High
Neuropathic pain 466 99.28 Very High Very High Very High
Dopamine 2 98.32 Very High Very High Very High
Lasting pain 37 95.64 Very High Very High Very High
Stimulus evoked pain 33 91.96 High High
Disease Link Frequency Relevance Heat
INFLAMMATION 330 100.00 Very High Very High Very High
Pain 1722 99.56 Very High Very High Very High
Ganglion Cysts 528 99.48 Very High Very High Very High
Neuropathic Pain 565 99.28 Very High Very High Very High
Cytomegalovirus Infection 66 99.04 Very High Very High Very High
Nervous System Injury 429 98.08 Very High Very High Very High
Toxicity 33 96.60 Very High Very High Very High
Neurodegenerative Disease 66 92.48 High High
Obesity 4 92.24 High High
Hypersensitivity 33 91.96 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
In this regard, further studies are warranted to investigate the mechanism by which activated microglia in spinal cord might be associated with GCH1 expression and then participate in the initial development and/or persistence of pain.
Gene_expression (expression) of GCH1 in microglia associated with pain, gch1 and spinal cord
1) Confidence 0.72 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 1.01 Pain Relevance 1.08
In immunohistochemistry, shGCH1 introduction via rAAV2 significantly suppressed GCH1 expression to a similar level as the no-pain control group, while the up-regulation of GCH1 remained unchanged in the rAAV-GFP injected group (Fig. 6a), which supports previous report showing the positive association of pain symptoms with GCH1 activation [10].
Gene_expression (expression) of GCH1 associated with pain and gch1
2) Confidence 0.72 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.76 Pain Relevance 1.10
One of the most significant findings of this study is the direct linkage of GCH1 expression alterations as underlying phenomena in animal models to genotypic characteristics within the human population.
Gene_expression (expression) of GCH1 associated with gch1
3) Confidence 0.72 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.85 Pain Relevance 1.24
These results collectively illustrate that rAAV-shGCH1 treatment of the sciatic nerve effectively suppresses GCH1 expression and leads to effective pain relief.
Gene_expression (expression) of GCH1 in sciatic nerve associated with pain, gch1 and sciatic nerve
4) Confidence 0.72 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.84 Pain Relevance 1.14
Our data collectively suggest that rAAV-mediated shGCH1 synthesis effectively suppresses GCH1 expression.


Gene_expression (expression) of GCH1 associated with gch1
5) Confidence 0.72 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.64 Pain Relevance 0.37
Similar pattern of GCH1 expression was also observed in western-blotting analysis (Fib. 6c).
Gene_expression (expression) of GCH1 associated with gch1
6) Confidence 0.72 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.73 Pain Relevance 1.06
In our study, a single injection of the virus led to consistent down-regulation of GCH1 expression following pain surgery, and improved pain resistance over time.
Gene_expression (expression) of GCH1 associated with pain and gch1
7) Confidence 0.72 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.91 Pain Relevance 0.88
Cultured ADSCs were retrovirally transduced with tyrosine hydroxylase (TH) and/or GTP cyclohydroxylase 1 (GCH1) genes and stably expressed them for at least 6 weeks in culture.
Gene_expression (transduced) of GCH1 associated with gch1
8) Confidence 0.63 Published 2009 Journal Cell Transplant Section Abstract Doc Link 19660177 Disease Relevance 0.47 Pain Relevance 0.57
When 0.5-1 x 10(6) TH- and GCH1-expressing ADSCs were intrathecally grafted in rats, elevated levels of levodopa and dopamine metabolites were found in CSF at 3 days, although at lower concentrations than expected.
Gene_expression (grafted) of GCH1-expressing in CSF associated with dopamine and gch1
9) Confidence 0.63 Published 2009 Journal Cell Transplant Section Abstract Doc Link 19660177 Disease Relevance 0.46 Pain Relevance 0.63
Our data collectively suggest that rAAV-mediated shGCH1 synthesis effectively suppresses GCH1 expression.


Gene_expression (synthesis) of shGCH1 associated with gch1
10) Confidence 0.63 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.62 Pain Relevance 0.36
For knockdown of rGCH1 expression, nine siRNAs targeting various rGCH1 exon regions were designed (Fig. 1).
Gene_expression (expression) of rGCH1
11) Confidence 0.63 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.43 Pain Relevance 0.61
To achieve persistent shRNA expression, we constructed rAAV-shGCH1 by inserting this shGCH1 expression cassette into the rAAV2 backbone. rAAV-shGCH1 additionally encodes the GFP reporter gene driven by the CMV promoter to allow the simultaneous tracking of shGCH1 expression (Fig. 2b). rAAV-GFP lacking the shGCH1 expression cassette was employed as a control.
Gene_expression (expression) of shGCH1 associated with cytomegalovirus infection
12) Confidence 0.63 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.39 Pain Relevance 0.35
The GCH1 silencing effect was examined using 293T cells exogenously expressing rGCH1, which were transfected with rGCH1-specific or control non-specific siRNA.
Gene_expression (expressing) of rGCH1 associated with gch1
13) Confidence 0.63 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.40 Pain Relevance 0.57
Western-blotting analysis revealed that all specific siRNAs markedly inhibited rGCH1 expression, while control siRNAs did not.
Gene_expression (expression) of rGCH1
14) Confidence 0.63 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.43 Pain Relevance 0.52
As expected, shGCH1 dramatically suppressed rGCH1 expression, similar to its corresponding siRNA (Fig. 2a).
Gene_expression (expression) of rGCH1
15) Confidence 0.63 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.39 Pain Relevance 0.45
The following antibodies were used: GCH1 (Abnova, Taiwan), ?
Gene_expression (used) of GCH1 associated with gch1
16) Confidence 0.62 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.55 Pain Relevance 0.45
The relative GCH1 levels were 2.3 ± 0.4 and 1.2 ± 0.3 in GFP- and shGHC1-treated pain groups, respectively (Fig. 6b).
Gene_expression (levels) of GCH1 associated with pain and gch1
17) Confidence 0.62 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 0.66 Pain Relevance 1.09
Attenuation of neuropathic pain behavior through downregulation of GCH1 levels via rAAV-shGCH1
Gene_expression (levels) of GCH1 associated with gch1 and neuropathic pain
18) Confidence 0.62 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 1.10 Pain Relevance 0.88
Subsequently reduced GCH1 expression was evident from immunohistochemistry and western-blotting analysis.
Gene_expression (expression) of GCH1 associated with gch1
19) Confidence 0.56 Published 2009 Journal Mol Pain Section Abstract Doc Link PMC2785765 Disease Relevance 0.60 Pain Relevance 0.97
Our present data indicates that blockage of GCH1 over-expression effectively attenuates pain symptoms, strongly supporting previous findings on the close correlation between GCH1 down-regulation and pain relief.
Gene_expression (expression) of GCH1 associated with pain and gch1
20) Confidence 0.56 Published 2009 Journal Mol Pain Section Body Doc Link PMC2785765 Disease Relevance 1.09 Pain Relevance 1.35

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