INT164087

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Context Info
Confidence 0.78
First Reported 2006
Last Reported 2010
Negated 0
Speculated 3
Reported most in Body
Documents 42
Total Number 45
Disease Relevance 5.39
Pain Relevance 3.21

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

isomerase activity (TPI1) cytosol (TPI1) small molecule metabolic process (TPI1)
nucleus (TPI1) embryo development (TPI1) carbohydrate metabolic process (TPI1)
Anatomy Link Frequency
Caco-2 4
upper 4
brain 1
platelet 1
TPI1 (Homo sapiens)
TPI1 - I170V (3)
Pain Link Frequency Relevance Heat
Fibrositis 10 100.00 Very High Very High Very High
qutenza 36 99.84 Very High Very High Very High
Serotonin 16 96.80 Very High Very High Very High
adenocard 4 92.72 High High
Pain 10 90.00 High High
cerebral cortex 1 79.20 Quite High
sSRI 6 71.36 Quite High
fluoxetine 3 60.96 Quite High
antagonist 3 47.64 Quite Low
Spontaneous pain 1 42.80 Quite Low
Disease Link Frequency Relevance Heat
Fatigue 11 100.00 Very High Very High Very High
Fibromyalgia 10 100.00 Very High Very High Very High
Sprains And Strains 895 99.30 Very High Very High Very High
Stress 348 96.64 Very High Very High Very High
Pain 12 90.00 High High
Nociception 2 83.28 Quite High
Disease 145 80.04 Quite High
Lifespan 5 73.60 Quite High
Polycystic Ovary Syndrome 4 57.92 Quite High
Aging 51 52.64 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Second, the reduced enzyme activities measured in the previous studies could be, at least in part, based on factors acting is trans; e.g. mutations affecting the activity of transcription factors controlling the expression of the TPI enzyme.
Gene_expression (expression) of TPI enzyme
1) Confidence 0.78 Published 2008 Journal BMC Genet Section Body Doc Link PMC2424074 Disease Relevance 0.25 Pain Relevance 0
However, yeast cells expressing the Ile170Val TPI variant showed a strong reduction in catalytic activity compared to the wild-type protein and the other pathogenic TPI variants.
Gene_expression (expressing) of TPI (I170V)
2) Confidence 0.77 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.15 Pain Relevance 0
Yeast cells co-expressing the fusion proteins LexA-TPI and AD-TPI2ndATG exhibited activity of all reporter genes demonstrating that the glycolytic inactive TPI2ndATG variant associates with wild-type TPI.
Gene_expression (co-expressing) of TPI
3) Confidence 0.75 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.07 Pain Relevance 0
Indeed, in the Eber et al. study, none of the heterozygous persons expressed an electrophoretic variant of the TPI enzyme [14].
Gene_expression (expressed) of TPI enzyme
4) Confidence 0.68 Published 2008 Journal BMC Genet Section Body Doc Link PMC2424074 Disease Relevance 0.28 Pain Relevance 0
The relative activity of the lacZ reporter gene measured in yeast co-expressing LexA-TPI in combination with AD-Gly122Arg or AD-Phe240Leu, respectively, was not significantly altered in comparison to yeast co-expressing LexA-TPI and AD-TPI suggesting that these mutations were not affecting the dimerization behavior with wild-type TPI (Fig. 5B, upper panel).
Gene_expression (expressing) of LexA-TPI in upper
5) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.08 Pain Relevance 0
The relative activity of the lacZ reporter gene measured in yeast co-expressing LexA-TPI in combination with AD-Gly122Arg or AD-Phe240Leu, respectively, was not significantly altered in comparison to yeast co-expressing LexA-TPI and AD-TPI suggesting that these mutations were not affecting the dimerization behavior with wild-type TPI (Fig. 5B, upper panel).
Gene_expression (expressing) of LexA-TPI in upper
6) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.09 Pain Relevance 0
Yeast co-expressing wild-type TPI and the different TPI2ndATG variants all displayed a reduced dimerization behavior in comparison to yeast cells expressing wild-type TPI and TPI2ndATG proteins.
Gene_expression (expressing) of wild-type TPI
7) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0 Pain Relevance 0
Overexpression of the Glu104Asp TPI variant results in reduced expression levels of endogenous TPI
Gene_expression (Overexpression) of TPI
8) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0 Pain Relevance 0
To rule out that a low intracellular protein level of the Ile170Val TPI variant causes this diminished activity in yeast, we analyzed the expression levels of the different TPI variants in yeast by immunoblot analyses.
Spec (analyzed) Gene_expression (expression) of TPI
9) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.13 Pain Relevance 0
This might explain why yeast cells expressing the TPI variant with reduced catalytic activity were not resistant to all oxidants examined.
Gene_expression (expressing) of TPI
10) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.26 Pain Relevance 0
Overexpression of the Glu104Asp TPI variant results in reduced expression levels of endogenous TPI
Gene_expression (expression) of TPI
11) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0 Pain Relevance 0
-galactosidase activity was observed in yeast co-expressing LexA-TPI and AD-Ile170Val, whereas a strong enhancement was measured in yeast expressing the fusion proteins LexA-TPI and AD-Cys41Tyr.
Gene_expression (expressing) of LexA-TPI
12) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.07 Pain Relevance 0
The relative activity of the lacZ reporter gene measured in yeast co-expressing LexA-TPI in combination with AD-Gly122Arg or AD-Phe240Leu, respectively, was not significantly altered in comparison to yeast co-expressing LexA-TPI and AD-TPI suggesting that these mutations were not affecting the dimerization behavior with wild-type TPI (Fig. 5B, upper panel).
Gene_expression (expressing) of AD-TPI in upper
13) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.08 Pain Relevance 0
Additionally, we demonstrated that the overexpression of the most frequent TPI variant, Glu104Asp, which displays altered dimerization features, results in diminished endogenous TPI levels in mammalian cells.
Gene_expression (overexpression) of TPI
14) Confidence 0.67 Published 2006 Journal PLoS ONE Section Abstract Doc Link PMC1762313 Disease Relevance 0.06 Pain Relevance 0
Regarding this issue, it is important that expression of the Ile170Val TPI variant with reduced catalytic activity results in higher resistance of yeast to diamide.
Gene_expression (expression) of TPI (I170V)
15) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.22 Pain Relevance 0
Strikingly, yeast cells expressing Ile170Val TPI, the variant exhibiting a reduced catalytic activity as shown in Fig. 3, were resistant to diamide treatment in comparison to strains expressing wild-type or the other pathogenic TPI variants (Fig. 7).
Gene_expression (expressing) of TPI (I170V) associated with sprains and strains
16) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.61 Pain Relevance 0
Strikingly, in comparison to overexpressed wild-type TPI, a strong reduction in the endogenous TPI level was detected in case the Glu104Asp variant was overexpressed (upper panel).
Gene_expression (overexpressed) of wild-type TPI in upper
17) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0 Pain Relevance 0
No activation of the reporter genes was observed in yeast cells expressing the fusion protein LexA-TPI and the AD domain alone demonstrating that complex formation of TPI occurs in the yeast two-hybrid system.
Gene_expression (expressing) of LexA-TPI
18) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.08 Pain Relevance 0
-galactosidase activity was observed in yeast co-expressing LexA-TPI and AD-Ile170Val, whereas a strong enhancement was measured in yeast expressing the fusion proteins LexA-TPI and AD-Cys41Tyr.
Gene_expression (expressing) of LexA-TPI
19) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.07 Pain Relevance 0
Yeast cells co-expressing the fusion proteins LexA-TPI and AD-TPI2ndATG exhibited activity of all reporter genes demonstrating that the glycolytic inactive TPI2ndATG variant associates with wild-type TPI.
Gene_expression (co-expressing) of LexA-TPI
20) Confidence 0.67 Published 2006 Journal PLoS ONE Section Body Doc Link PMC1762313 Disease Relevance 0.07 Pain Relevance 0

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