INT164209

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Context Info
Confidence 0.77
First Reported 2008
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 3
Total Number 20
Disease Relevance 5.90
Pain Relevance 0.32

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

transport (Slc5a8) plasma membrane (Slc5a8) transmembrane transport (Slc5a8)
Anatomy Link Frequency
colon 3
MCF7 3
epithelial cells 2
neurons 2
retina 1
Slc5a8 (Mus musculus)
Pain Link Frequency Relevance Heat
Bioavailability 54 93.12 High High
cINOD 54 72.16 Quite High
member 8 2 50.00 Quite Low
Neurotransmitter 18 40.04 Quite Low
Glutamate 18 38.92 Quite Low
Disease Link Frequency Relevance Heat
Malignant Neoplastic Disease 144 99.84 Very High Very High Very High
Apoptosis 270 99.24 Very High Very High Very High
Cancer 758 94.88 High High
Breast Cancer 36 92.84 High High
Inflammatory Bowel Disease 18 83.52 Quite High
Colon Cancer 92 72.64 Quite High
INFLAMMATION 54 71.76 Quite High
Diabetes Mellitus 18 55.12 Quite High
Starvation 18 52.88 Quite High
Targeted Disruption 18 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
There is no overlap between SLC5A8 expression pattern and that of MCT1 (an astrocyte-specific MCT) and GFAP (a glial cell marker).
Gene_expression (expression) of SLC5A8 in astrocyte
1) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0 Pain Relevance 0
A similar expression pattern of SLC5A8 and SLC5A12 is also seen in the retina (26).
Gene_expression (expression) of SLC5A8 in retina
2) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0.06 Pain Relevance 0
When SLC5A8 is expressed ectopically in malignant cells, there is no effect as long as the culture medium does not contain butyrate, showing that the expression of the transporter by itself alone is insufficient to induce apoptosis.
Gene_expression (expressed) of SLC5A8 associated with malignant neoplastic disease and apoptosis
3) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 1.25 Pain Relevance 0
SLC5A8 is expressed primarily in neurons and in the retinal pigment epithelium whereas SLC5A12 is expressed almost exclusively in Müller cells, the glial cells of the retina.
Gene_expression (expressed) of SLC5A8 in epithelium
4) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0.12 Pain Relevance 0
In the kidney, SLC5A8 is expressed in the apical membrane of tubular epithelial cells and the expression is restricted principally to the straight portion of the proximal tubule (S3 segment) (14).
Gene_expression (expressed) of SLC5A8 in kidney
5) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0 Pain Relevance 0
The expression of SLC5A8 in the apical membrane of these cells suggests that the transporter may be involved in facilitating the cellular entry of this drug.
Gene_expression (expression) of SLC5A8
6) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0.14 Pain Relevance 0.09
Therefore, we asked if SLC5A8, a highly concentrative transporter for lactate and ketone bodies because of its coupling to transmembrane Na+ gradient and the electrogenic nature of the transport process, is expressed in neurons.
Gene_expression (expressed) of SLC5A8 in neurons
7) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0.11 Pain Relevance 0
Therefore, a MCF7 cell line engineered to express SLC5A8 stably would be ideal for the screening.
Gene_expression (express) of SLC5A8 in MCF7
8) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0.92 Pain Relevance 0
The expression pattern of SLC5A8 in the brain overlaps with that of MCT2 (a neuron-specific MCT), NeuN (a neuronal cell body marker) and MAP2 (a neuronal axonal marker).
Gene_expression (expression) of SLC5A8 in neuronal
9) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0 Pain Relevance 0
SLC5A8 is expressed differentially in the lumen-facing apical membrane of colonic and intestinal epithelial cells (15,16).
Gene_expression (expressed) of SLC5A8 in epithelial cells
10) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0 Pain Relevance 0
Supplementation of the culture medium with pyruvate does not do any harm to MCF7 cells because of the lack of expression of SLC5A8; but ectopic expression of the transporter in these cells leads to apoptosis when cultured in the presence of pyruvate-supplemented culture medium.
Gene_expression (expression) of SLC5A8 in MCF7 associated with apoptosis
11) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0.93 Pain Relevance 0
In studies by Li et al. (3), ectopic expression of SLC5A8 in malignant colon cells led to growth arrest, but there was no evidence of addition of butyrate to the culture medium in these experiments.
Gene_expression (expression) of SLC5A8 in colon associated with malignant neoplastic disease
12) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0.67 Pain Relevance 0
EXPRESSION PATTERN OF SLC5A8 AND SLC5A12 IN THE INTESTINAL TRACT AND KIDNEY
Gene_expression (EXPRESSION) of SLC5A8
13) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0 Pain Relevance 0
The expression of SLC5A8 in the lumen-facing apical membrane of epithelial cells lining the small intestine, colon, and the renal proximal tubule indicates that the transporter may influence oral bioavailability and renal reabsorption of monocarboxylate drugs.
Gene_expression (expression) of SLC5A8 in lumen associated with bioavailability
14) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0.94 Pain Relevance 0.12
In the regular medium generally recommended for the culture of MCF7 cells, ectopic expression of SLC5A8 invariably led to apoptosis, suggesting that the culture medium used in these studies also contained the ‘mystery’ substrate.
Gene_expression (expression) of SLC5A8 in MCF7 associated with apoptosis
15) Confidence 0.77 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0.78 Pain Relevance 0
These data show that SLC5A8 offers a Na+-coupled electrogenic transport system for ?
Gene_expression (offers) of SLC5A8
16) Confidence 0.67 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0 Pain Relevance 0.07
These studies show that the high-affinity sodium-coupled MCT SLC5A8 is expressed specifically in neurons in the brain, suggesting that the transporter might play an important role in the neuronal uptake of lactate and ketone bodies and thus in the maintenance of energy status of the neurons.
Gene_expression (expressed) of SLC5A8 in neurons
17) Confidence 0.60 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0 Pain Relevance 0
-Hydroxybutyrate (25 nM) Was Measured in a NaCl Buffer in Vector-transfected Cells and SLC5A8 cDNA-transfected Cells in the Presence or Absence of Unlabeled Monocarboxylate Substrates (5 mM) of the TransporterFig. 2Transport of ?
Gene_expression (cDNA) of SLC5A8
18) Confidence 0.58 Published 2008 Journal AAPS J Section Body Doc Link PMC2751467 Disease Relevance 0 Pain Relevance 0.04
Consistently, Slc5a8 expression was significantly reduced in colons of Cdx1(-/-) or Cdx2(+/-) mice compared with wild-type mice.
Gene_expression (expression) of Slc5a8 in colons
19) Confidence 0.53 Published 2010 Journal Gastroenterology Section Body Doc Link 19900445 Disease Relevance 0 Pain Relevance 0
Overexpression of Cdx1 or Cdx2 in human colon cancer cell lines induced expression of endogenous SLC5A8, whereas CDX1 and CDX2 knockdowns reduced its level.
Gene_expression (expression) of SLC5A8 in colon
20) Confidence 0.46 Published 2010 Journal Gastroenterology Section Body Doc Link 19900445 Disease Relevance 0 Pain Relevance 0

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